PMID- 35871846
OWN - NLM
STAT- MEDLINE
DCOM- 20220726
LR  - 20220801
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 670
DP  - 2022
TI  - Purification and use of carotenoid standards to quantify cis-trans geometrical 
      carotenoid isomers in plant tissues.
PG  - 57-85
LID - S0076-6879(22)00005-2 [pii]
LID - 10.1016/bs.mie.2022.01.005 [doi]
AB  - Reverse-phase high-performance liquid chromatography (HPLC) is a preferred method 
      used to identify and quantify carotenoids. Here, we describe a straightforward, 
      reliable, and cost-effective protocol to purify and develop individual carotenoid 
      standards for absolute quantification of carotenoids, including selected 
      cis-trans (geometric) isomers. Analytical techniques to extract, purify and 
      collect individual carotenoids using an HPLC system equipped with a Diode Array 
      Detector (DAD) and fraction collector are described. Carotenoids were separated 
      and identified by their characteristic ultraviolet-visible (UV-Vis) absorption 
      spectra and individually isolated based on their retention times using a C30 
      column. This chapter outlines how to prepare standard calibration curves using 
      known quantities of purified and/or commercially available carotenoids. A series 
      of molar extinction and slope coefficients for phytoene, phytofluene, zeta-carotene, 
      neurosporene, pro-lycopene, all trans-lycopene, lutein, beta-carotene, zeaxanthin, 
      antheraxanthin, violaxanthin, neoxanthin, capsanthin, capsorubin and 
      beta-cryptoxanthin are defined to enable absolute quantification of their abundance 
      in plant, animal, and bacterial tissues. Different approaches for reporting 
      carotenoid abundance by absolute concentration, relative composition, and/or 
      using ratios of different pigments are provided as a convenient resource for 
      carotenoid researchers.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Anwar, Sidra
AU  - Anwar S
AD  - Hawkesbury Institute for the Environment, Hawkesbury Campus, Western Sydney 
      University, Richmond, NSW, Australia.
FAU - Nayak, Jwalit J
AU  - Nayak JJ
AD  - Hawkesbury Institute for the Environment, Hawkesbury Campus, Western Sydney 
      University, Richmond, NSW, Australia.
FAU - Alagoz, Yagiz
AU  - Alagoz Y
AD  - Hawkesbury Institute for the Environment, Hawkesbury Campus, Western Sydney 
      University, Richmond, NSW, Australia; National Vegetable Protected Cropping 
      Centre, Hawkesbury Campus, Western Sydney University, Richmond, NSW, Australia.
FAU - Wojtalewicz, Dominika
AU  - Wojtalewicz D
AD  - Hawkesbury Institute for the Environment, Hawkesbury Campus, Western Sydney 
      University, Richmond, NSW, Australia.
FAU - Cazzonelli, Christopher I
AU  - Cazzonelli CI
AD  - Hawkesbury Institute for the Environment, Hawkesbury Campus, Western Sydney 
      University, Richmond, NSW, Australia; National Vegetable Protected Cropping 
      Centre, Hawkesbury Campus, Western Sydney University, Richmond, NSW, Australia. 
      Electronic address: c.cazzonelli@westernsydney.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220131
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 36-88-4 (Carotenoids)
SB  - IM
MH  - Animals
MH  - *Carotenoids
MH  - Chromatography, High Pressure Liquid/methods
MH  - Isomerism
MH  - Reference Standards
OTO - NOTNLM
OT  - Carotenoid
OT  - Concentration
OT  - Geometric isomers
OT  - HPLC
OT  - Metabolite
OT  - Plant pigment
OT  - Slope coefficient
OT  - Standard curve
OT  - Xanthophyll
OT  - cis-Carotene
EDAT- 2022/07/26 06:00
MHDA- 2022/07/27 06:00
CRDT- 2022/07/25 01:39
PHST- 2022/07/25 01:39 [entrez]
PHST- 2022/07/26 06:00 [pubmed]
PHST- 2022/07/27 06:00 [medline]
AID - S0076-6879(22)00005-2 [pii]
AID - 10.1016/bs.mie.2022.01.005 [doi]
PST - ppublish
SO  - Methods Enzymol. 2022;670:57-85. doi: 10.1016/bs.mie.2022.01.005. Epub 2022 Jan 
      31.