PMID- 35872930
OWN - NLM
STAT- MEDLINE
DCOM- 20220726
LR  - 20230811
IS  - 1748-6718 (Electronic)
IS  - 1748-670X (Print)
IS  - 1748-670X (Linking)
VI  - 2022
DP  - 2022
TI  - TPPU Downregulates Oxidative Stress Damage and Induces BDNF Expression in PC-12 
      Cells.
PG  - 7083022
LID - 10.1155/2022/7083022 [doi]
LID - 7083022
AB  - OBJECTIVE: Ischemia-reperfusion is an ongoing clinical challenge that can lead to 
      a series of pathological changes including oxidative stress. The inhibition of 
      soluble epoxide hydrolase inhibitor (sEH) by 
      1-(1-propanoylpiperidin-4-yl)-3-[4-(trifluoromethoxy)phenyl]urea (TPPU) results 
      in an anti-inflammatory, cardioprotective, and blood vessel growth-promoting 
      effects. Therefore, this study focused on the protective effect of TPPU on a rat 
      pheochromocytoma (PC-12) cell oxidative stress model induced by H(2)O(2). 
      METHODS: CCK-8 and Hoechst 33342 were used to evaluate cell apoptosis and western 
      blot to detect the apoptotic proteins and brain-derived neurotrophic factor 
      (BDNF) expression. RESULT: The incubation with 100 muM, 50 muM, and 25 muM TPPU 
      significantly increased PC-12 cell viability. Epoxyeicosatrienoic acid (EET) 
      pretreatment also protected PC-12 cells from oxidative stress. In addition, TPPU 
      reduced caspase-3 and Bax expression and induced Bcl-2 expression, and EETs 
      exerted the same effect on caspase-3 expression as TPPU. A positive relationship 
      was found between TPPU or EET incubation and BDNF expression. CONCLUSION: These 
      results revealed that TPPU reduced PC-12 cell oxidative stress injury induced by 
      H(2)O(2) and promoted BDNF expression.
CI  - Copyright (c) 2022 Qiong Wu et al.
FAU - Wu, Qiong
AU  - Wu Q
AD  - Department of Basic Medicine, Guangdong Jiangmen Chinese Medicine College, 
      Jiangmen, Guangdong Province, China 529000.
FAU - Lin, Minlin
AU  - Lin M
AD  - Department of Basic Medicine, Guangdong Jiangmen Chinese Medicine College, 
      Jiangmen, Guangdong Province, China 529000.
FAU - Wu, Peng
AU  - Wu P
AD  - Department of General Surgery, Jiangmen Wuyi Hospital of TCM, Jiangmen, Guangdong 
      Province, China 529000.
FAU - Zhao, Chongyan
AU  - Zhao C
AD  - Department of Basic Medicine, Guangdong Jiangmen Chinese Medicine College, 
      Jiangmen, Guangdong Province, China 529000.
FAU - Yang, Shuang
AU  - Yang S
AD  - Department of General Surgery, Jiangmen Wuyi Hospital of TCM, Jiangmen, Guangdong 
      Province, China 529000.
FAU - Yu, Haiying
AU  - Yu H
AD  - Department of Basic Medicine, Guangdong Jiangmen Chinese Medicine College, 
      Jiangmen, Guangdong Province, China 529000.
FAU - Xian, Wenjiao
AU  - Xian W
AD  - Department of Basic Medicine, Guangdong Jiangmen Chinese Medicine College, 
      Jiangmen, Guangdong Province, China 529000.
FAU - Song, Jingfang
AU  - Song J
AUID- ORCID: 0000-0003-2381-8801
AD  - Department of General Surgery, Jiangmen Wuyi Hospital of TCM, Jiangmen, Guangdong 
      Province, China 529000.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20220713
PL  - United States
TA  - Comput Math Methods Med
JT  - Computational and mathematical methods in medicine
JID - 101277751
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Phenylurea Compounds)
RN  - 0 (Piperidines)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
RIN - Comput Math Methods Med. 2023 Aug 2;2023:9863231. PMID: 37564774
MH  - Animals
MH  - *Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Caspase 3/metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - *Epoxide Hydrolases/genetics/metabolism
MH  - Hydrogen Peroxide/toxicity
MH  - Oxidative Stress
MH  - Phenylurea Compounds/pharmacology/therapeutic use
MH  - Piperidines/pharmacology/therapeutic use
MH  - Rats
PMC - PMC9300306
COIS- The authors declare that they have no conflict of interests.
EDAT- 2022/07/26 06:00
MHDA- 2022/07/27 06:00
PMCR- 2022/07/13
CRDT- 2022/07/25 03:27
PHST- 2022/06/01 00:00 [received]
PHST- 2022/06/22 00:00 [revised]
PHST- 2022/06/27 00:00 [accepted]
PHST- 2022/07/25 03:27 [entrez]
PHST- 2022/07/26 06:00 [pubmed]
PHST- 2022/07/27 06:00 [medline]
PHST- 2022/07/13 00:00 [pmc-release]
AID - 10.1155/2022/7083022 [doi]
PST - epublish
SO  - Comput Math Methods Med. 2022 Jul 13;2022:7083022. doi: 10.1155/2022/7083022. 
      eCollection 2022.