PMID- 35875188
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220930
IS  - 0167-6857 (Print)
IS  - 1573-5044 (Electronic)
IS  - 0167-6857 (Linking)
VI  - 151
IP  - 1
DP  - 2022
TI  - Biosynthetic studies through feeding experiments in Eclipta prostrata (L.) L. 
      hairy roots.
PG  - 215-219
LID - 10.1007/s11240-022-02342-0 [doi]
AB  - Eclipta prostrata (L.) L. is a medicinal plant of the Asteraceae family, and 
      several extracts and isolated compounds of E. prostrata (L.) L. showed a wide 
      range of biological activities such as antimicrobial, anticancer, 
      hepatoprotective, neuroprotective, hair growth promoting activities, and more 
      recently against covid-19. Eclipta prostrata (L.) L. hairy roots produce 
      wedelolactone (WL), demethylwedelolactone (DWL) and 3,5-di-O-caffeoylquinic acid 
      (3,5-diCQA), and there is no data in literature regarding biosynthetic pathways 
      are involved. To verify the biosynthetic route, feeding experiments were carried 
      out using sodium [2-(13)C]acetate, [3-(13)C]dl-phenylalanine, and (13)C-labeled 
      compounds (WL, DWL and 3,5-diCQA) were detected by ultra-high-performance liquid 
      chromatography-quadrupole time of flight mass spectrometry (HPLC-QTOF-MS). 
      Analysis showed that the metabolic pathways operative of coumestans (WL and DWL) 
      are derived from acetate and shikimate pathways, while that the phenylpropanoid 
      (3,5-diCQA) biosynthesis is exclusively from shikimate pathway. SUPPLEMENTARY 
      INFORMATION: The online version contains supplementary material available at 
      10.1007/s11240-022-02342-0.
CI  - (c) The Author(s), under exclusive licence to Springer Nature B.V. 2022.
FAU - Lopes, Adriana A
AU  - Lopes AA
AD  - Biotechnology Unit, Universidade de Ribeirao Preto (UNAERP), Ribeirao Preto, SP 
      Brazil. GRID: grid.412281.c. ISNI: 0000 0000 8810 9529
FAU - Souza, Giuliana R S
AU  - Souza GRS
AD  - Biotechnology Unit, Universidade de Ribeirao Preto (UNAERP), Ribeirao Preto, SP 
      Brazil. GRID: grid.412281.c. ISNI: 0000 0000 8810 9529
FAU - de Castro Franca, Suzelei
AU  - de Castro Franca S
AD  - Biotechnology Unit, Universidade de Ribeirao Preto (UNAERP), Ribeirao Preto, SP 
      Brazil. GRID: grid.412281.c. ISNI: 0000 0000 8810 9529
FAU - Lourenco, Miriam V
AU  - Lourenco MV
AD  - Biotechnology Unit, Universidade de Ribeirao Preto (UNAERP), Ribeirao Preto, SP 
      Brazil. GRID: grid.412281.c. ISNI: 0000 0000 8810 9529
LA  - eng
PT  - Journal Article
DEP - 20220717
PL  - Netherlands
TA  - Plant Cell Tissue Organ Cult
JT  - Plant cell, tissue and organ culture
JID - 9879654
PMC - PMC9288585
OTO - NOTNLM
OT  - Acetate pathway
OT  - Asteraceae
OT  - Coumestans
OT  - Phenylpropanoid
OT  - Shikimate pathway
COIS- Conflict of  interestThe authors declare no conflict of interest.
EDAT- 2022/07/26 06:00
MHDA- 2022/07/26 06:01
PMCR- 2022/07/17
CRDT- 2022/07/25 04:06
PHST- 2022/04/07 00:00 [received]
PHST- 2022/06/15 00:00 [accepted]
PHST- 2022/07/26 06:00 [pubmed]
PHST- 2022/07/26 06:01 [medline]
PHST- 2022/07/25 04:06 [entrez]
PHST- 2022/07/17 00:00 [pmc-release]
AID - 2342 [pii]
AID - 10.1007/s11240-022-02342-0 [doi]
PST - ppublish
SO  - Plant Cell Tissue Organ Cult. 2022;151(1):215-219. doi: 
      10.1007/s11240-022-02342-0. Epub 2022 Jul 17.