PMID- 35875537
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230916
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - Diversity and Ecophysiology of the Genus OLB8 and Other Abundant Uncultured 
      Saprospiraceae Genera in Global Wastewater Treatment Systems.
PG  - 917553
LID - 10.3389/fmicb.2022.917553 [doi]
LID - 917553
AB  - The Saprospiraceae family within the phylum Bacteroidota is commonly present and 
      highly abundant in wastewater treatment plants (WWTPs) worldwide, but little is 
      known about its role. In this study, we used MiDAS 4 global survey with samples 
      from 30 countries to analyze the abundance and distribution of members of 
      Saprospiraceae. Phylogenomics were used to delineate five new genera from a set 
      of 31 high-quality metagenome-assembled genomes from Danish WWTPs. Newly designed 
      probes for fluorescence in situ hybridization (FISH) revealed rod-shaped 
      morphologies for all genera analyzed, including OLB8, present mostly inside the 
      activated sludge flocs. The genomes revealed potential metabolic capabilities for 
      the degradation of polysaccharides, proteins, and other complex molecules; 
      partial denitrification; and storage of intracellular polymers (glycogen, 
      polyphosphate, and polyhydroxyalkanoates). FISH in combination with Raman 
      microspectroscopy confirmed the presence of intracellular glycogen in Candidatus 
      Brachybacter, Candidatus Parvibacillus calidus (both from the former genus OLB8), 
      and Candidatus Opimibacter, and the presence of polyhydroxyalkanoates in 
      Candidatus Defluviibacterium haderslevense and Candidatus Vicinibacter. These 
      results provide the first overview of the most abundant novel Saprospiraceae 
      genera present in WWTPs across the world and their potential involvement in 
      nutrient removal and the degradation of macromolecules.
CI  - Copyright (c) 2022 Kondrotaite, Valk, Petriglieri, Singleton, Nierychlo, Dueholm 
      and Nielsen.
FAU - Kondrotaite, Zivile
AU  - Kondrotaite Z
AD  - Department of Chemistry and Bioscience, Center of Microbial Communities, Aalborg 
      University, Aalborg, Denmark.
FAU - Valk, Laura C
AU  - Valk LC
AD  - Department of Chemistry and Bioscience, Center of Microbial Communities, Aalborg 
      University, Aalborg, Denmark.
FAU - Petriglieri, Francesca
AU  - Petriglieri F
AD  - Department of Chemistry and Bioscience, Center of Microbial Communities, Aalborg 
      University, Aalborg, Denmark.
FAU - Singleton, Caitlin
AU  - Singleton C
AD  - Department of Chemistry and Bioscience, Center of Microbial Communities, Aalborg 
      University, Aalborg, Denmark.
FAU - Nierychlo, Marta
AU  - Nierychlo M
AD  - Department of Chemistry and Bioscience, Center of Microbial Communities, Aalborg 
      University, Aalborg, Denmark.
FAU - Dueholm, Morten K D
AU  - Dueholm MKD
AD  - Department of Chemistry and Bioscience, Center of Microbial Communities, Aalborg 
      University, Aalborg, Denmark.
FAU - Nielsen, Per H
AU  - Nielsen PH
AD  - Department of Chemistry and Bioscience, Center of Microbial Communities, Aalborg 
      University, Aalborg, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20220708
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9304909
OTO - NOTNLM
OT  - activated sludge
OT  - FISH-Raman
OT  - MiDAS
OT  - OLB8
OT  - Saprospiraceae
OT  - high-quality MAGs
OT  - wastewater
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/26 06:00
MHDA- 2022/07/26 06:01
PMCR- 2022/07/08
CRDT- 2022/07/25 04:12
PHST- 2022/04/11 00:00 [received]
PHST- 2022/05/30 00:00 [accepted]
PHST- 2022/07/25 04:12 [entrez]
PHST- 2022/07/26 06:00 [pubmed]
PHST- 2022/07/26 06:01 [medline]
PHST- 2022/07/08 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.917553 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Jul 8;13:917553. doi: 10.3389/fmicb.2022.917553. 
      eCollection 2022.