PMID- 35876627
OWN - NLM
STAT- MEDLINE
DCOM- 20220809
LR  - 20221117
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 14
IP  - 14
DP  - 2022 Jul 21
TI  - Moxibustion exhibits therapeutic effects on spinal cord injury via modulating 
      microbiota dysbiosis and macrophage polarization.
PG  - 5800-5811
LID - 10.18632/aging.204184 [doi]
AB  - In this study, we aimed to study the effect of moxibustion (MOX) on microbiota 
      dysbiosis and macrophage polarization, so as to unveil the mechanism underlying 
      the therapeutic effect of MOX in the management of spinal cord injury (SCI). SCI 
      animal models were established to study the effect of MOX. Accordingly, it was 
      found that MOX treatment significantly suppressed the Ace index and Shannon index 
      in the SCI group. Moreover, the reduced relative levels of Lactobacillales and 
      Bifidobacteriales and the elevated relative level of Clostridiales in the SCI 
      animals were mitigated by the treatment of MOX. The body weight, food intake, 
      energy expenditure (EE) index and respiratory quotient (RQ) index of SCI mice 
      were all evidently decreased, but the levels of interleukin (IL)-17, interferon 
      (IFN)-gamma, monocyte chemoattractant protein-1 (MCP-1) and IL-1beta were increased in 
      the SCI group. Moreover, MOX treatment significantly mitigated the dysregulation 
      of above factors in SCI mice. Accordingly, we found that the Basso Mouse Scale 
      (BMS) score was negatively correlated with the level of Clostridiales while 
      positively correlated with the level of Lactobacillales. The apoptotic index and 
      caspase-3 level were both evidently increased in the SCI group, while the SCI+MOX 
      group showed reduced levels of apoptotic index and caspase-3. Therefore, it can 
      be concluded that the treatment with MOX can promote microbiota dysbiosis and 
      macrophage polarization, thus alleviating spinal cord injury by down-regulating 
      the expression of inflammatory cytokines.
FAU - Zhang, Zhuang
AU  - Zhang Z
AD  - Department of Neurology, The First Affiliated Hospital, Jinzhou Medical 
      University, Jinzhou, Liaoning 121012, China.
FAU - Sui, Rubo
AU  - Sui R
AD  - Department of Neurology, The First Affiliated Hospital, Jinzhou Medical 
      University, Jinzhou, Liaoning 121012, China.
FAU - Ge, Lili
AU  - Ge L
AD  - Department of Ultrasound, The First Affiliated Hospital, Jinzhou Medical 
      University, Jinzhou, Liaoning 121012, China.
FAU - Xia, Dongjian
AU  - Xia D
AD  - Department of Neurosurgery, The First Affiliated Hospital, Jinzhou Medical 
      University, Jinzhou, Liaoning 121012, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220721
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Caspase 3/metabolism
MH  - Dysbiosis/metabolism/therapy
MH  - Macrophages/metabolism
MH  - Mice
MH  - *Microbiota
MH  - *Moxibustion
MH  - *Spinal Cord Injuries/metabolism/therapy
PMC - PMC9365548
OTO - NOTNLM
OT  - inflammation
OT  - macrophage polarization
OT  - microbiota dysbiosis
OT  - moxibustion
OT  - spinal cord injury
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2022/07/26 06:00
MHDA- 2022/08/10 06:00
PMCR- 2022/07/31
CRDT- 2022/07/25 09:42
PHST- 2022/04/04 00:00 [received]
PHST- 2022/06/14 00:00 [accepted]
PHST- 2022/07/26 06:00 [pubmed]
PHST- 2022/08/10 06:00 [medline]
PHST- 2022/07/25 09:42 [entrez]
PHST- 2022/07/31 00:00 [pmc-release]
AID - 204184 [pii]
AID - 10.18632/aging.204184 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2022 Jul 21;14(14):5800-5811. doi: 10.18632/aging.204184. Epub 
      2022 Jul 21.