PMID- 35880571
OWN - NLM
STAT- MEDLINE
DCOM- 20220727
LR  - 20230211
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Print)
IS  - 1672-9145 (Linking)
VI  - 54
IP  - 7
DP  - 2022 Jun 25
TI  - Circular RNA-VPS13A attenuates diabetes-induced enteric glia damage by targeting 
      miR-182/GDNF Axis.
PG  - 999-1007
LID - 10.3724/abbs.2022073 [doi]
AB  - Gastrointestinal (GI) complications of diabetes mellitus (DM) significantly 
      impact on patients' quality of life. Enteric glial cells (EGC) are the key cell 
      type of enteric nervous system (ENS), which contributes to the destruction of gut 
      homeostasis in DM. Circular RNAs (circRNAs) are a novel type of RNAs abundant in 
      the eukaryotic transcriptome, which form covalently closed continuous loops. In 
      this study, the contribution of circRNAs to EGC damage in DM is investigated. 
      Transcriptome sequencing analysis and functional study show that circVPS13A is 
      significantly down-regulated in hyperglycemia-treated EGC, and circVPS13A 
      overexpression attenuates EGC damage in both in vitro and in vivo DM models. In 
      vitro mechanistic study using dual-luciferase reporter assay, affinity-isolation 
      assay, fluorescence in situ hybridization (FISH) and immunostaining analysis 
      identify that circVPS13A exerts its protective effect by sponging miR-182 and 
      then up-regulates glial cell line-derived neurotrophic factor (GDNF) expression. 
      In addition, in vivo study confirms that the circVPS13A-miR-182-GDNF network 
      regulation can attenuate hyperglycemia-induced EGC damage of duodenum in 
      streptozotocine (STZ)-induced DM mice. The findings of this study may provide 
      novel insights into the protective role of circVPS13A in DM-associated EGC damage 
      and clues for the development of new therapeutic approaches for the prevention of 
      GI complications of DM.
FAU - Zhu, Xiaowei
AU  - Zhu X
AD  - Department of Endocrinology and Metabolism, the First Affiliated Hospital of 
      Nanjing Medical University, Nanjing 210029, China.
AD  - Department of Endocrinology, Wuxi People's Hospital Affiliated to Nanjing Medical 
      University, Wuxi 214000, China.
FAU - Li, Yanyu
AU  - Li Y
AD  - Department of Endocrinology, Wuxi People's Hospital Affiliated to Nanjing Medical 
      University, Wuxi 214000, China.
FAU - Zhu, Xuping
AU  - Zhu X
AD  - Department of Endocrinology, Wuxi People's Hospital Affiliated to Nanjing Medical 
      University, Wuxi 214000, China.
FAU - Wang, Ke
AU  - Wang K
AD  - NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular 
      Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China.
FAU - Zhu, Xue
AU  - Zhu X
AD  - NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular 
      Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi 214063, China.
FAU - Jiang, Yanmin
AU  - Jiang Y
AD  - Department of Endocrinology, Wuxi People's Hospital Affiliated to Nanjing Medical 
      University, Wuxi 214000, China.
FAU - Xu, Lan
AU  - Xu L
AD  - Department of Endocrinology, Wuxi People's Hospital Affiliated to Nanjing Medical 
      University, Wuxi 214000, China.
FAU - Li, Jianbo
AU  - Li J
AD  - Department of Endocrinology and Metabolism, the First Affiliated Hospital of 
      Nanjing Medical University, Nanjing 210029, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Experimental/complications/genetics/metabolism
MH  - Glial Cell Line-Derived Neurotrophic Factor/genetics
MH  - *Hyperglycemia/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - *MicroRNAs/metabolism
MH  - Neuroglia
MH  - Quality of Life
MH  - RNA, Circular/genetics
PMC - PMC9828216
OTO - NOTNLM
OT  - circVPS13A
OT  - diabetes mellitus
OT  - enteric glia
OT  - gastrointestinal complications
COIS- The authors declare that they have no conflict of interest.
EDAT- 2022/07/27 06:00
MHDA- 2022/07/28 06:00
PMCR- 2022/07/08
CRDT- 2022/07/26 05:32
PHST- 2022/07/26 05:32 [entrez]
PHST- 2022/07/27 06:00 [pubmed]
PHST- 2022/07/28 06:00 [medline]
PHST- 2022/07/08 00:00 [pmc-release]
AID - 10.3724/abbs.2022073 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2022 Jun 25;54(7):999-1007. doi: 
      10.3724/abbs.2022073.