PMID- 35882114
OWN - NLM
STAT- MEDLINE
DCOM- 20220810
LR  - 20220810
IS  - 2772-9508 (Electronic)
IS  - 2772-9508 (Linking)
VI  - 139
DP  - 2022 Aug
TI  - Engineered artificial articular cartilage made of decellularized extracellular 
      matrix by mechanical and IGF-1 stimulation.
PG  - 213019
LID - S2772-9508(22)00296-5 [pii]
LID - 10.1016/j.bioadv.2022.213019 [doi]
AB  - Cartilage engineering has the potential to overcome clinical deficiency in joint 
      disorders. Decellularized extracellular matrix (dECM) has great biocompatibility 
      and bioactivity and can be considered an appropriate natural scaffold for tissue 
      engineering applications. Both insulin-like growth factor-1 (IGF-1) and 
      mechanical compression stimulate the production of cartilage ECM, modulate 
      mechanical properties, and gene expression. The current investigation aimed to 
      fabricate a high-quality moldable artificial cartilage by exposing the 
      chondrocytes in biomimicry conditions using cartilage dECM, IGF-1, and mechanical 
      stimulations. In this study, an ad hoc bioreactor was designed to apply dynamic 
      mechanical stimuli (10 % strain, 1 Hz) on chondrocyte-laden cartilage 
      dECM-constructs with/without IGF-1 supplementation for 2 weeks, 3 h/day. Our data 
      revealed that mechanical stimulation had no adverse effect on cell viability and 
      proliferation. However, it elevated the expression of chondrogenic markers such 
      as collagen type II (COL2A1), aggrecan (ACAN), and proteoglycan-4 (PRG-4), and 
      reduced the expression of matrix metalloproteinase-3 (MMP-3). Mechanical 
      stimulation also promoted higher newly formed glycosaminoglycan (GAG) and 
      produced more aligned fibers that can be responsible for higher Young's modulus 
      of the engineered construct. Even though IGF-1 demonstrated some extent of 
      improvement in developing neocartilage, it was not as effective as mechanical 
      stimulation. Neither IGF-1 nor compression elevated the collagen type I 
      expression. Compression and IGF-1 showed a synergistic impact on boosting the 
      level of COL2A1 but not the other factors. In conclusion, mechanical stimulation 
      on moldable cartilage dECM can be considered a good technique to fabricate 
      artificial cartilage with higher functionality.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Sani, Mahsa
AU  - Sani M
AD  - Tissue Engineering Department, School of Advanced Medical Science and Technology, 
      Shiraz University of Medical Science, Shiraz, Iran. Electronic address: 
      sani_mahsa@yahoo.com.
FAU - Hosseinie, Radmarz
AU  - Hosseinie R
AD  - Department of Mechanical Engineering College of Engineering, Fasa University, 
      Fasa, Iran.
FAU - Latifi, Mona
AU  - Latifi M
AD  - Tissue Engineering Lab, Department of Anatomical Sciences, School of Medicine, 
      Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Shadi, Mehri
AU  - Shadi M
AD  - Tissue Engineering Lab, Department of Anatomical Sciences, School of Medicine, 
      Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Razmkhah, Mahboobeh
AU  - Razmkhah M
AD  - Tissue Engineering Department, School of Advanced Medical Science and Technology, 
      Shiraz University of Medical Science, Shiraz, Iran; Shiraz Institute for Cancer 
      Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, 
      Iran. Electronic address: razmkhahm@sums.ac.ir.
FAU - Salmannejad, Mahin
AU  - Salmannejad M
AD  - Tissue Engineering Lab, Department of Anatomical Sciences, School of Medicine, 
      Shiraz University of Medical Sciences, Shiraz, Iran.
FAU - Parsaei, Hossein
AU  - Parsaei H
AD  - Department of Medical Physics and Engineering, School of Medicine, Shiraz 
      University of Medical Sciences, Shiraz, Iran.
FAU - Talaei-Khozani, Tahereh
AU  - Talaei-Khozani T
AD  - Tissue Engineering Department, School of Advanced Medical Science and Technology, 
      Shiraz University of Medical Science, Shiraz, Iran; Tissue Engineering Lab, 
      Department of Anatomical Sciences, School of Medicine, Shiraz University of 
      Medical Sciences, Shiraz, Iran; Histomorphometry and Stereology Research center, 
      Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran. 
      Electronic address: talaeit@sums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20220708
PL  - Netherlands
TA  - Biomater Adv
JT  - Biomaterials advances
JID - 9918383886206676
RN  - 0 (Decellularized Extracellular Matrix)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - *Cartilage, Articular/metabolism
MH  - Chondrocytes/metabolism
MH  - Decellularized Extracellular Matrix
MH  - Insulin-Like Growth Factor I/genetics
MH  - Tissue Engineering/methods
OTO - NOTNLM
OT  - Bioreactor
OT  - Compression
OT  - Decellularized extracellular matrix
OT  - Insulin-like growth factor-1
EDAT- 2022/07/27 06:00
MHDA- 2022/08/11 06:00
CRDT- 2022/07/26 18:17
PHST- 2022/04/20 00:00 [received]
PHST- 2022/06/19 00:00 [revised]
PHST- 2022/07/03 00:00 [accepted]
PHST- 2022/07/27 06:00 [pubmed]
PHST- 2022/08/11 06:00 [medline]
PHST- 2022/07/26 18:17 [entrez]
AID - S2772-9508(22)00296-5 [pii]
AID - 10.1016/j.bioadv.2022.213019 [doi]
PST - ppublish
SO  - Biomater Adv. 2022 Aug;139:213019. doi: 10.1016/j.bioadv.2022.213019. Epub 2022 
      Jul 8.