PMID- 35885901
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20220929
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 13
IP  - 7
DP  - 2022 Jun 22
TI  - The Genetic Diversity of Bletilla spp. Based on SLAF-seq and Oligo-FISH.
LID - 10.3390/genes13071118 [doi]
LID - 1118
AB  - Bletilla spp. Rchb. F. is a traditional Chinese medicinal material. In this 
      study, Bletilla striata (Thunb. ex A. Murray) Rchb F, Bletilla formosana (Hayata) 
      Schltr, and Bletilla ochracea Schltr were collected to analyze the genetic 
      diversity of 16 materials using specific site-amplified fragment sequencing 
      (SLAF-seq) and fluorescence in situ hybridization (FISH). The results showed that 
      the phylogenetic tree of the single-nucleotide polymorphism (SNP) data rendering 
      system was correlated with the shape and geographical distribution of the 
      material. The results of the population structural analysis showed that all the 
      materials containing yellow labellum came from the same ancestor. The results of 
      the principal component analysis were able to preliminarily judge the genetic 
      distance and provided a reference for the selection of hybrid parents. The FISH 
      analysis showed that the chromosomes of B. striata were 2n = 32 and the 
      chromosomes of the B. striata (safflower) mutant were 2n = 34 and the chromosomes 
      of B. ochracea and B. formosana were 2n = 34-36. The (AG(3)T(3))(3) non-terminal 
      signal was different from the 5S rDNA signal. These results revealed that the 16 
      materials had rich genetic diversity, which can provide molecular and cytogenetic 
      data for the study of the genus and its relatives and serve as a reference for 
      the breeding of new genus varieties and improve breeding efficiency and cost.
FAU - Huan, Jie
AU  - Huan J
AD  - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang 
      District, Chengdu 611130, China.
AD  - Institute of Forestry, Chengdu Academy of Agriculture and Forestry Sciences, 
      Nongke Road 200, Wenjiang District, Chengdu 611130, China.
FAU - He, Zhoujian
AU  - He Z
AUID- ORCID: 0000-0002-8980-8107
AD  - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang 
      District, Chengdu 611130, China.
FAU - Lei, Yuting
AU  - Lei Y
AD  - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang 
      District, Chengdu 611130, China.
FAU - Li, Wenjun
AU  - Li W
AD  - Institute of Forestry, Chengdu Academy of Agriculture and Forestry Sciences, 
      Nongke Road 200, Wenjiang District, Chengdu 611130, China.
FAU - Jiang, Liqiong
AU  - Jiang L
AD  - Institute of Forestry, Chengdu Academy of Agriculture and Forestry Sciences, 
      Nongke Road 200, Wenjiang District, Chengdu 611130, China.
FAU - Luo, Xiaomei
AU  - Luo X
AUID- ORCID: 0000-0001-5943-0150
AD  - College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang 
      District, Chengdu 611130, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220622
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
SB  - IM
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *Orchidaceae
MH  - Phylogeny
MH  - *Plant Breeding
MH  - Polymorphism, Single Nucleotide/genetics
PMC - PMC9318928
OTO - NOTNLM
OT  - Bletilla
OT  - SLAF
OT  - molecular cytogenetics
OT  - oligo-FISH
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/29 06:00
PMCR- 2022/06/22
CRDT- 2022/07/27 01:17
PHST- 2022/04/29 00:00 [received]
PHST- 2022/06/14 00:00 [revised]
PHST- 2022/06/20 00:00 [accepted]
PHST- 2022/07/27 01:17 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/06/22 00:00 [pmc-release]
AID - genes13071118 [pii]
AID - genes-13-01118 [pii]
AID - 10.3390/genes13071118 [doi]
PST - epublish
SO  - Genes (Basel). 2022 Jun 22;13(7):1118. doi: 10.3390/genes13071118.