PMID- 35886967
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20221207
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 14
DP  - 2022 Jul 10
TI  - The Phagocytosis of Lacticaseibacillus casei and Its Immunomodulatory Properties 
      on Human Monocyte-Derived Dendritic Cells Depend on the Expression of Lc-p75, a 
      Bacterial Peptidoglycan Hydrolase.
LID - 10.3390/ijms23147620 [doi]
LID - 7620
AB  - The human gut symbiont Lacticaseibacillus (L.) casei (previously Lactobacillus 
      casei) is under intense research due to its wide range of immunomodulatory 
      effects on the human host. Dendritic cells (DCs) are crucial players in the 
      direct and indirect communication with lactobacilli in the gastrointestinal 
      tract. Here, we demonstrate that human monocyte-derived DCs (moDCs) are able to 
      engulf L. casei BL23, in which the intact bacterial cell wall and morphology have 
      a key role. The absence of the bacterial cell-wall-degrading enzyme, Lc-p75, in 
      L. casei cells causes remarkable morphological changes, which have important 
      consequences in the phagocytosis of L. casei by moDCs. Our results showed that 
      the Lc-p75 mutation induced defective internalization and impaired 
      proinflammatory and T-cell-polarizing cytokine secretion by bacteria-exposed 
      moDCs. The T helper (Th) 1 and Th17 cell activating capacity of moDCs induced by 
      the mutant L. casei was consequently reduced. Moreover, inhibition of the 
      phagocytosis of wild-type bacteria showed similar results. Taken together, these 
      data suggested that formation of short bacterial chains helps to exert the potent 
      immunomodulatory properties of L. casei BL23.
FAU - Toth, Marta
AU  - Toth M
AUID- ORCID: 0000-0003-1007-0232
AD  - Department of Immunology, Faculty of Medicine, University of Debrecen, 4032 
      Debrecen, Hungary.
AD  - Doctoral School of Molecular Cell and Immune Biology, University of Debrecen, 
      4032 Debrecen, Hungary.
AD  - ELKH-DE Allergology Research Group, 4032 Debrecen, Hungary.
FAU - Muzsai, Szabolcs
AU  - Muzsai S
AD  - Department of Immunology, Faculty of Medicine, University of Debrecen, 4032 
      Debrecen, Hungary.
AD  - Gyula Petranyi Doctoral School of Clinical Immunology and Allergology, University 
      of Debrecen, 4032 Debrecen, Hungary.
FAU - Regulski, Krzysztof
AU  - Regulski K
AD  - INRAE, AgroParisTech, Micalis Institute, Universite Paris-Saclay, 78350 
      Jouy-en-Josas, France.
FAU - Szendi-Szatmari, Timea
AU  - Szendi-Szatmari T
AD  - Department of Biophysics and Cell Biology, Faculty of Medicine, University of 
      Debrecen, 4032 Debrecen, Hungary.
FAU - Czimmerer, Zsolt
AU  - Czimmerer Z
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, University 
      of Debrecen, 4032 Debrecen, Hungary.
FAU - Rajnavolgyi, Eva
AU  - Rajnavolgyi E
AD  - Department of Immunology, Faculty of Medicine, University of Debrecen, 4032 
      Debrecen, Hungary.
FAU - Chapot-Chartier, Marie-Pierre
AU  - Chapot-Chartier MP
AUID- ORCID: 0000-0002-4947-0519
AD  - INRAE, AgroParisTech, Micalis Institute, Universite Paris-Saclay, 78350 
      Jouy-en-Josas, France.
FAU - Bacsi, Attila
AU  - Bacsi A
AUID- ORCID: 0000-0002-2311-2975
AD  - Department of Immunology, Faculty of Medicine, University of Debrecen, 4032 
      Debrecen, Hungary.
AD  - ELKH-DE Allergology Research Group, 4032 Debrecen, Hungary.
LA  - eng
GR  - TAMOP-4.2.2.A-11/1/KONV-2012 0023/New Hungary Development Plan/
GR  - NK 101538/National Research, Development and Innovation Office/
GR  - NN 114423/National Research, Development and Innovation Office/
GR  - K 125337/National Research, Development and Innovation Office/
GR  - GINOP-2.3.2-15-2016-00050/Hungarian Government Szechenyi 2020 Program/
PT  - Journal Article
DEP - 20220710
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - EC 3.5.1.28 (N-Acetylmuramoyl-L-alanine Amidase)
SB  - IM
MH  - *Dendritic Cells/immunology
MH  - Humans
MH  - *Lacticaseibacillus casei/genetics/immunology/physiology
MH  - Monocytes/immunology
MH  - *N-Acetylmuramoyl-L-alanine Amidase/biosynthesis/genetics/immunology
MH  - Phagocytosis
PMC - PMC9319067
OTO - NOTNLM
OT  - dendritic cells
OT  - immunomodulation
OT  - lactobacillus
OT  - phagocytosis
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/29 06:00
PMCR- 2022/07/10
CRDT- 2022/07/27 01:22
PHST- 2022/05/25 00:00 [received]
PHST- 2022/07/02 00:00 [revised]
PHST- 2022/07/07 00:00 [accepted]
PHST- 2022/07/27 01:22 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/07/10 00:00 [pmc-release]
AID - ijms23147620 [pii]
AID - ijms-23-07620 [pii]
AID - 10.3390/ijms23147620 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Jul 10;23(14):7620. doi: 10.3390/ijms23147620.