PMID- 35886978
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20220802
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 14
DP  - 2022 Jul 10
TI  - Evaluation of the Impact of Pregnancy-Associated Factors on the Quality of 
      Wharton's Jelly-Derived Stem Cells Using SOX2 Gene Expression as a Marker.
LID - 10.3390/ijms23147630 [doi]
LID - 7630
AB  - SOX2 is a recognized pluripotent transcription factor involved in stem cell 
      homeostasis, self-renewal and reprogramming. It belongs to, one of the 
      SRY-related HMG-box (SOX) family of transcription factors, taking part in the 
      regulation of embryonic development and determination of cell fate. Among other 
      functions, SOX2 promotes proliferation, survival, invasion, metastasis, cancer 
      stemness, and drug resistance. SOX2 interacts with other transcription factors in 
      multiple signaling pathways to control growth and survival. The aim of the study 
      was to determine the effect of a parturient's age, umbilical cord blood pH and 
      length of pregnancy on the quality of stem cells derived from Wharton's jelly 
      (WJSC) by looking at birth weight and using SOX2 gene expression as a marker. 
      Using qPCR the authors, evaluated the expression of SOX2 in WJSC acquired from 
      the umbilical cords of 30 women right after the delivery. The results showed a 
      significant correlation between the birth weight and the expression of SOX2 in 
      WJSC in relation to maternal age, umbilical cord blood pH, and the length of 
      pregnancy. The authors observed that the younger the woman and the lower the 
      umbilical cord blood pH, the earlier the delivery occurs, the lower the birth 
      weight and the higher SOX2 gene expression in WJSC. In research studies and 
      clinical applications of regenerative medicine utilizing mesenchymal stem cells 
      derived from Wharton's Jelly of the umbilical cord, assessment of maternal and 
      embryonic factors influencing the quality of cells is critical.
FAU - Gil-Kulik, Paulina
AU  - Gil-Kulik P
AUID- ORCID: 0000-0003-3119-981X
AD  - Department of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska 
      Str., 20-080 Lublin, Poland.
FAU - Swistowska, Malgorzata
AU  - Swistowska M
AUID- ORCID: 0000-0001-9366-3556
AD  - Department of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska 
      Str., 20-080 Lublin, Poland.
FAU - Krzyzanowski, Arkadiusz
AU  - Krzyzanowski A
AUID- ORCID: 0000-0003-1147-3359
AD  - Department of Obstetrics and Pathology of Pregnancy, Medical University of 
      Lublin, 11 Staszica Str., 20-081 Lublin, Poland.
FAU - Petniak, Alicja
AU  - Petniak A
AUID- ORCID: 0000-0001-9926-0870
AD  - Department of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska 
      Str., 20-080 Lublin, Poland.
FAU - Kwasniewska, Anna
AU  - Kwasniewska A
AUID- ORCID: 0000-0003-2553-8635
AD  - Department of Obstetrics and Pathology of Pregnancy, Medical University of 
      Lublin, 11 Staszica Str., 20-081 Lublin, Poland.
FAU - Plachno, Bartosz J
AU  - Plachno BJ
AUID- ORCID: 0000-0001-5579-5101
AD  - Department of Plant Cytology and Embryology, Institute of Botany, Faculty of 
      Biology, Jagiellonian University in Krakow, 9 Gronostajowa St., 30-387 Cracow, 
      Poland.
FAU - Galkowski, Dariusz
AU  - Galkowski D
AD  - Pathology and Laboratory Medicine, Rutgers Robert Wood Johnson Medical School, 
      New Brunswick, NJ 08901, USA.
FAU - Bogucka-Kocka, Anna
AU  - Bogucka-Kocka A
AUID- ORCID: 0000-0001-8473-7429
AD  - Chair and Department of Biology and Genetics, Medical University of Lublin, 4a 
      Chodzki St., 20-093 Lublin, Poland.
FAU - Kocki, Janusz
AU  - Kocki J
AUID- ORCID: 0000-0002-9541-6353
AD  - Department of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska 
      Str., 20-080 Lublin, Poland.
LA  - eng
GR  - "The equipment of innovative laboratories doing research on new medicines used in 
      the therapy of civilization and neoplastic diseases"/Operational Program 
      Development of Eastern Poland 2007-2013, Priority Axis I Modern Economy, 
      Operations I.3 Innovation Promotion/
GR  - STRATEGMED III (STRATEGMED3/303570/7/NCBR/2017)/National Centre for Research and 
      Development/
PT  - Journal Article
DEP - 20220710
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Birth Weight
MH  - Cell Differentiation/physiology
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Infant, Newborn
MH  - *Mesenchymal Stem Cells/metabolism/physiology
MH  - Pregnancy
MH  - *SOXB1 Transcription Factors/genetics/metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - *Wharton Jelly/cytology/metabolism
PMC - PMC9317592
OTO - NOTNLM
OT  - SOX2
OT  - WJSC
OT  - gene expression
OT  - mesenchymal stem cells
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/29 06:00
PMCR- 2022/07/10
CRDT- 2022/07/27 01:22
PHST- 2022/06/06 00:00 [received]
PHST- 2022/07/06 00:00 [revised]
PHST- 2022/07/08 00:00 [accepted]
PHST- 2022/07/27 01:22 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/07/10 00:00 [pmc-release]
AID - ijms23147630 [pii]
AID - ijms-23-07630 [pii]
AID - 10.3390/ijms23147630 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Jul 10;23(14):7630. doi: 10.3390/ijms23147630.