PMID- 35888939
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220731
IS  - 2072-666X (Print)
IS  - 2072-666X (Electronic)
IS  - 2072-666X (Linking)
VI  - 13
IP  - 7
DP  - 2022 Jul 15
TI  - Fabrication of a Cell-Friendly Poly(dimethylsiloxane) Culture Surface via 
      Polydopamine Coating.
LID - 10.3390/mi13071122 [doi]
LID - 1122
AB  - In this study, we fabricated a poly(dimethylsiloxane) (PDMS) surface coated with 
      polydopamine (PDA) to enhance cell adhesion. PDA is well known for improving 
      surface adhesion on various surfaces due to the abundant reactions enabled by the 
      phenyl, amine, and catechol groups contained within it. To confirm the successful 
      surface coating with PDA, the water contact angle and X-ray photoelectron 
      spectroscopy were analyzed. Human umbilical vein endothelial cells (HUVECs) and 
      human-bone-marrow-derived mesenchymal stem cells (MSCs) were cultured on the 
      PDA-coated PDMS surface to evaluate potential improvements in cell adhesion and 
      proliferation. HUVECs were also cultured inside a cylindrical PDMS microchannel, 
      which was constructed to mimic a human blood vessel, and their growth and 
      performance were compared to those of cells grown inside a rectangular 
      microchannel. This study provides a helpful perspective for building a platform 
      that mimics in vivo environments in a more realistic manner.
FAU - Yang, Da Hyun
AU  - Yang DH
AD  - Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, 
      Sujeong-gu, Seongnam-si 13120, Korea.
FAU - Jung, Sangyong
AU  - Jung S
AUID- ORCID: 0000-0003-3017-1145
AD  - Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology 
      and Research (A*STAR), Singapore 138667, Singapore.
AD  - Department of Physiology, Yong Loo Lin School of Medicine, National University of 
      Singapore, Singapore 117593, Singapore.
FAU - Kim, Jae Young
AU  - Kim JY
AUID- ORCID: 0000-0001-9323-3580
AD  - Department of Life Science, Gachon University, 1342 Seongnam-daero, Sujeong-gu, 
      Seongnam-si 13120, Korea.
FAU - Lee, Nae Yoon
AU  - Lee NY
AUID- ORCID: 0000-0001-5029-4009
AD  - Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, 
      Sujeong-gu, Seongnam-si 13120, Korea.
LA  - eng
GR  - NRF 2020R1A2B5B01001971/National Research Foundation of Korea/
GR  - GCU-202002700001/Gachon University/
PT  - Journal Article
DEP - 20220715
PL  - Switzerland
TA  - Micromachines (Basel)
JT  - Micromachines
JID - 101640903
PMC - PMC9315764
OTO - NOTNLM
OT  - cell adhesion
OT  - human umbilical vein endothelial cells
OT  - mesenchymal stem cells
OT  - poly(dimethylsiloxane)
OT  - polydopamine
OT  - vascular mimetics
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/28 06:01
PMCR- 2022/07/15
CRDT- 2022/07/27 01:32
PHST- 2022/06/03 00:00 [received]
PHST- 2022/06/24 00:00 [revised]
PHST- 2022/07/12 00:00 [accepted]
PHST- 2022/07/27 01:32 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/28 06:01 [medline]
PHST- 2022/07/15 00:00 [pmc-release]
AID - mi13071122 [pii]
AID - micromachines-13-01122 [pii]
AID - 10.3390/mi13071122 [doi]
PST - epublish
SO  - Micromachines (Basel). 2022 Jul 15;13(7):1122. doi: 10.3390/mi13071122.