PMID- 35889181
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220731
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 10
IP  - 7
DP  - 2022 Jul 20
TI  - In Vitro and In Silico Analysis of the Inhibitory Activity of EGCG-Stearate 
      against Herpes Simplex Virus-2.
LID - 10.3390/microorganisms10071462 [doi]
LID - 1462
AB  - About half a billion people worldwide are infected with herpes simplex virus-2 
      (HSV-2). Prolonged treatment with acyclovir (ACV) and its analogs leads to the 
      development of resistant strains. The aim of this study was to investigate the 
      antiviral potential of epigallocatechin gallate (EGCG) from Camellia sinensis and 
      a stable analog EGCG-stearate (EGCG-S) against HSV-2 in cultured Vero cells. Cell 
      viability and cell proliferation assays were used to determine the non-cytotoxic 
      concentrations on cultured Vero cells. HSV-2 with a green fluorescent protein 
      (GFP) fusion protein of VP26 virions were treated with non-cytotoxic 
      concentrations of EGCG and EGCG-S. The effects on infectivity and mechanisms were 
      determined by plaque assay, attachment and penetration assays, confocal 
      microscopy, qPCR, and in silico modeling analysis. Our results demonstrate that 
      treatment of HSV-2 virions with EGCG and EGCG-S at a concentration of 75 microM 
      showed greater than 99.9% inhibition by inhibiting the attachment of HSV-2 
      virions to host cells. The bioinformatic analysis indicated high binding affinity 
      of EGCG-S for glycoprotein D; thus EGCG-S may block fusion of HSV-2 and the cell 
      membrane, preventing entry of HSV-2 into the cell.
FAU - Stamos, James D
AU  - Stamos JD
AD  - Biology Department, Montclair State University, Montclair, NJ 07043, USA.
FAU - Lee, Lee H
AU  - Lee LH
AUID- ORCID: 0000-0002-5203-6000
AD  - Biology Department, Montclair State University, Montclair, NJ 07043, USA.
FAU - Taylor, Calvin
AU  - Taylor C
AD  - Biology Department, Montclair State University, Montclair, NJ 07043, USA.
FAU - Elias, Tony
AU  - Elias T
AD  - Biology Department, Montclair State University, Montclair, NJ 07043, USA.
FAU - Adams, Sandra D
AU  - Adams SD
AUID- ORCID: 0000-0003-4260-0576
AD  - Biology Department, Montclair State University, Montclair, NJ 07043, USA.
LA  - eng
PT  - Journal Article
DEP - 20220720
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC9315604
OTO - NOTNLM
OT  - EGCG
OT  - EGCG-stearate
OT  - HSV-2
OT  - antiviral
OT  - bioinformatics
OT  - qPCR
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/28 06:01
PMCR- 2022/07/20
CRDT- 2022/07/27 01:33
PHST- 2022/06/16 00:00 [received]
PHST- 2022/07/17 00:00 [revised]
PHST- 2022/07/18 00:00 [accepted]
PHST- 2022/07/27 01:33 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/28 06:01 [medline]
PHST- 2022/07/20 00:00 [pmc-release]
AID - microorganisms10071462 [pii]
AID - microorganisms-10-01462 [pii]
AID - 10.3390/microorganisms10071462 [doi]
PST - epublish
SO  - Microorganisms. 2022 Jul 20;10(7):1462. doi: 10.3390/microorganisms10071462.