PMID- 35889190
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220731
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 10
IP  - 7
DP  - 2022 Jul 20
TI  - iChip-Inspired Isolation, Bioactivities and Dereplication of Actinomycetota from 
      Portuguese Beach Sediments.
LID - 10.3390/microorganisms10071471 [doi]
LID - 1471
AB  - Oceans hold a stunning number of unique microorganisms, which remain unstudied by 
      culture-dependent methods due to failures in establishing the right conditions 
      for these organisms to grow. In this work, an isolation effort inspired by the 
      iChip was performed using marine sediments from Memoria beach, Portugal. The 
      isolates obtained were identified by 16S rRNA gene analysis, fingerprinted using 
      BOX-PCR and ERIC-PCR, searched for the putative presence of secondary metabolism 
      genes associated with polyketide synthase I (PKS-I) and non-ribosomal peptide 
      synthetases (NRPS), screened for antimicrobial activity against Escherichia coli 
      ATCC 25922 and Staphylococcus aureus ATCC 29213, and had bioactive extracts 
      dereplicated by LC/HRMS. Of the 158 isolated strains, 96 were affiliated with the 
      phylum Actinomycetota, PKS-I and NRPS genes were detected in 53 actinomycetotal 
      strains, and 11 proved to be bioactive (10 against E. coli, 1 against S. aureus 
      and 1 against both pathogens). Further bioactivities were explored using an "one 
      strain many compounds" approach, with six strains showing continued bioactivity 
      and one showing a novel one. Extract dereplication showed the presence of several 
      known bioactive molecules and potential novel ones in the bioactive extracts. 
      These results indicate the use of the bacteria isolated here as sources of new 
      bioactive natural products.
FAU - Dos Santos, Jose Diogo Neves
AU  - Dos Santos JDN
AUID- ORCID: 0000-0002-9693-4857
AD  - Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo 
      Alegre S/N, 4169-007 Porto, Portugal.
AD  - Interdisciplinary Centre of Marine and Environmental Research, University of 
      Porto, Terminal de Cruzeiros do Porto de Leixoes, Avenida General Norton de 
      Matos, S/N, 4450-208 Matosinhos, Portugal.
FAU - Joao, Susana Afonso
AU  - Joao SA
AD  - Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo 
      Alegre S/N, 4169-007 Porto, Portugal.
AD  - Interdisciplinary Centre of Marine and Environmental Research, University of 
      Porto, Terminal de Cruzeiros do Porto de Leixoes, Avenida General Norton de 
      Matos, S/N, 4450-208 Matosinhos, Portugal.
FAU - Martin, Jesus
AU  - Martin J
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Avenida del Conocimiento, 34 Parque Tecnologico de 
      Ciencias de la Salud, 18016 Granada, Spain.
FAU - Vicente, Francisca
AU  - Vicente F
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Avenida del Conocimiento, 34 Parque Tecnologico de 
      Ciencias de la Salud, 18016 Granada, Spain.
FAU - Reyes, Fernando
AU  - Reyes F
AUID- ORCID: 0000-0003-1607-5106
AD  - Fundacion MEDINA, Centro de Excelencia en Investigacion de Medicamentos 
      Innovadores en Andalucia, Avenida del Conocimiento, 34 Parque Tecnologico de 
      Ciencias de la Salud, 18016 Granada, Spain.
FAU - Lage, Olga Maria
AU  - Lage OM
AUID- ORCID: 0000-0002-3950-3970
AD  - Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo 
      Alegre S/N, 4169-007 Porto, Portugal.
AD  - Interdisciplinary Centre of Marine and Environmental Research, University of 
      Porto, Terminal de Cruzeiros do Porto de Leixoes, Avenida General Norton de 
      Matos, S/N, 4450-208 Matosinhos, Portugal.
LA  - eng
GR  - UIDB/04423/2020/Fundacao para a Ciencia e Tecnologia/
GR  - UIDP/04423/2020/Fundacao para a Ciencia e Tecnologia/
GR  - SFRH/BD/145576/2019/Fundacao para a Ciencia e Tecnologia/
PT  - Journal Article
DEP - 20220720
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC9319460
OTO - NOTNLM
OT  - LC/HRMS
OT  - antimicrobial activities
OT  - iChip
OT  - marine Actinomycetota
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/28 06:01
PMCR- 2022/07/20
CRDT- 2022/07/27 01:33
PHST- 2022/07/01 00:00 [received]
PHST- 2022/07/13 00:00 [revised]
PHST- 2022/07/19 00:00 [accepted]
PHST- 2022/07/27 01:33 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/28 06:01 [medline]
PHST- 2022/07/20 00:00 [pmc-release]
AID - microorganisms10071471 [pii]
AID - microorganisms-10-01471 [pii]
AID - 10.3390/microorganisms10071471 [doi]
PST - epublish
SO  - Microorganisms. 2022 Jul 20;10(7):1471. doi: 10.3390/microorganisms10071471.