PMID- 35889219 OWN - NLM STAT- MEDLINE DCOM- 20220728 LR - 20220731 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 27 IP - 14 DP - 2022 Jul 6 TI - Extracting Total Anthocyanin from Purple Sweet Potato Using an Effective Ultrasound-Assisted Compound Enzymatic Extraction Technology. LID - 10.3390/molecules27144344 [doi] LID - 4344 AB - This study aimed to develop an effective technique for extracting total anthocyanins from purple sweet potato (Mianzishu 9) (PSP9) by ultrasound-assisted compound enzymatic extraction (UAEE). Single-factor experiments, Plackett-Burman experimental design, and response surface methodology were utilized for optimizing extraction conditions, and the antioxidant activities were evaluated. Anthocyanins were also measured using an ultra-performance liquid chromatograph linked to a mass spectrometer (UPLC-MS). The maximum yield of total anthocyanins was 2.27 mg/g under the following conditions: the ethanol concentration was 78%, the material-to-liquid ratio was 1:15 g/mL, the enzyme ratio (cellulase: pectinase: papain) was 2:2:1 and its hydrolysis was at 41 degrees C, pH = 4.5, 1.5 h, the ultrasonication was at 48 degrees C and conducted twice for 20 min each time. In addition to higher yield, anthocyanins extracted from purple sweet potato by UAEE showed great ability to scavenge DPPH (IC(50) of 0.089 mug/mL) and hydroxyl radicals (IC(50) of 100.229 mug/mL). Five anthocyanins were found in the purple sweet potato extract from UAEE. Taken together, the ultrasound-assisted compound enzymatic method can rapidly and effectively extract anthocyanins with greater antioxidant capacity from purple sweet potato. FAU - Wang, Fang AU - Wang F AD - Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China. FAU - Zhang, Shuo AU - Zhang S AD - Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China. FAU - Deng, Guowei AU - Deng G AUID- ORCID: 0000-0001-7330-5874 AD - Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China. FAU - Xu, Kun AU - Xu K AD - Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, China. FAU - Xu, Haiyan AU - Xu H AD - College of Life Sciences, Sichuan Normal University, Chengdu 610101, China. FAU - Liu, Jialei AU - Liu J AUID- ORCID: 0000-0002-0227-5302 AD - Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China. LA - eng GR - 2018JY0441/Sichuan Science and Technology Program/ GR - CS18SD05/University-level project of Chengdu Normal University/ PT - Journal Article DEP - 20220706 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Anthocyanins) RN - 0 (Antioxidants) RN - 0 (Plant Extracts) SB - IM MH - Anthocyanins/chemistry MH - Antioxidants/pharmacology MH - Chromatography, Liquid MH - *Ipomoea batatas/chemistry MH - Plant Extracts/chemistry/pharmacology MH - Tandem Mass Spectrometry MH - Technology PMC - PMC9317032 OTO - NOTNLM OT - anthocyanins OT - antioxidant activity OT - purple sweet potato OT - response surface methodology OT - ultrasound-assisted compound enzymatic extraction COIS- The authors declare no conflict of interest. EDAT- 2022/07/28 06:00 MHDA- 2022/07/29 06:00 PMCR- 2022/07/06 CRDT- 2022/07/27 01:33 PHST- 2022/06/10 00:00 [received] PHST- 2022/07/01 00:00 [revised] PHST- 2022/07/04 00:00 [accepted] PHST- 2022/07/27 01:33 [entrez] PHST- 2022/07/28 06:00 [pubmed] PHST- 2022/07/29 06:00 [medline] PHST- 2022/07/06 00:00 [pmc-release] AID - molecules27144344 [pii] AID - molecules-27-04344 [pii] AID - 10.3390/molecules27144344 [doi] PST - epublish SO - Molecules. 2022 Jul 6;27(14):4344. doi: 10.3390/molecules27144344.