PMID- 35889753
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20220731
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 14
IP  - 14
DP  - 2022 Jul 7
TI  - Exogenous Nucleotides Improved the Oxidative Stress and Sirt-1 Protein Level of 
      Brown Adipose Tissue on Senescence-Accelerated Mouse Prone-8 (SAMP8) Mice.
LID - 10.3390/nu14142796 [doi]
LID - 2796
AB  - Brown adipose tissue (BAT) is of great importance in rodents for maintaining 
      their core temperature via non-shivering thermogenesis in the mitochondria. BAT's 
      thermogenic function has been shown to decline with age. The activation of 
      adenosine 5'-monophosphate (AMP)-activated protein kinase/sirtuin-1 (AMPK/Sirt-1) 
      is effective in regulating mitochondrial function. Exogenous nucleotides (NTs) 
      are regulatory factors in many biological processes. Nicotinamide mononucleotide 
      (NMN), which is a derivative of NTs, is widely known as a Sirt-1 activator in 
      liver and muscle, but the effect of NMN and NTs on aging BAT has not been studied 
      before. The purpose of this study was to investigate the effect of NTs on aging 
      senescence-accelerated mouse prone-8 (SAMP8) mice. Senescence-accelerated mouse 
      resistant 1 (SAMR1) mice were set as the model control group and NMN was used as 
      the positive control. Male, 3 month old SAMP8 mice were divided into the 
      SAMP8-normal chow (SAMP8-NC), SAMP8-young-normal chow (SAMP8-young-NC), NMN, 
      NTs-free, NTs-low, NTs-medium, and NTs-high groups for long-term feeding. After 9 
      months of intervention, interscapular BAT was collected for experiments. Compared 
      to the SAMP8-NC, the body weight and BAT mass were significantly improved in the 
      NT-treated aging SAMP8 mice. NT supplementation had effects on oxidative stress 
      in BAT. The concentration of malondialdehyde (MDA) was reduced and that of 
      superoxide dismutase (SOD) increased significantly. Meanwhile, the expression of 
      the brown adipocyte markers uncoupling protein-1 (UCP-1), peroxisome 
      proliferator-activated receptor-gamma coactlvator-1alpha (PGC-1alpha), and PR domain zinc 
      finger protein 16 (PRDM16) were upregulated. The upregulated proteins may be 
      activated via the Sirt-1 pathway. Thus, NT supplementation may be helpful to 
      improve the thermogenesis of BAT by reducing oxidative stress and activating the 
      Sirt-1 pathway.
FAU - Wang, Xiujuan
AU  - Wang X
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Peking 
      University, Beijing 100191, China.
FAU - Liu, Rui
AU  - Liu R
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Peking 
      University, Beijing 100191, China.
FAU - Wei, Chan
AU  - Wei C
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Peking 
      University, Beijing 100191, China.
FAU - Xu, Meihong
AU  - Xu M
AUID- ORCID: 0000-0001-7201-6982
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Peking 
      University, Beijing 100191, China.
FAU - Li, Yong
AU  - Li Y
AUID- ORCID: 0000-0003-3914-5840
AD  - Department of Nutrition and Food Hygiene, School of Public Health, Peking 
      University, Beijing 100191, China.
LA  - eng
PT  - Journal Article
DEP - 20220707
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Nucleotides)
RN  - 0 (Transcription Factors)
RN  - EC 3.5.1.- (Sirtuins)
SB  - IM
MH  - *Adipose Tissue, Brown/metabolism
MH  - Aging/metabolism
MH  - Animals
MH  - Male
MH  - Mice
MH  - Nucleotides/pharmacology
MH  - Oxidative Stress
MH  - *Sirtuins/metabolism
MH  - Thermogenesis
MH  - Transcription Factors/metabolism
PMC - PMC9320366
OTO - NOTNLM
OT  - AMPK/Sirt-1
OT  - aging
OT  - brown adipose tissue
OT  - nucleotides
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/29 06:00
PMCR- 2022/07/07
CRDT- 2022/07/27 01:36
PHST- 2022/03/30 00:00 [received]
PHST- 2022/06/24 00:00 [revised]
PHST- 2022/07/01 00:00 [accepted]
PHST- 2022/07/27 01:36 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/07/07 00:00 [pmc-release]
AID - nu14142796 [pii]
AID - nutrients-14-02796 [pii]
AID - 10.3390/nu14142796 [doi]
PST - epublish
SO  - Nutrients. 2022 Jul 7;14(14):2796. doi: 10.3390/nu14142796.