PMID- 35890047
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220810
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 11
IP  - 7
DP  - 2022 Jul 16
TI  - Establishing a Herpesvirus Quiescent Infection in Differentiated Human Dorsal 
      Root Ganglion Neuronal Cell Line Mediated by Micro-RNA Overexpression.
LID - 10.3390/pathogens11070803 [doi]
LID - 803
AB  - HSV-1 is a neurotropic pathogen associated with severe encephalitis, excruciating 
      orofacial sensation, and other chronic neuropathic complications. After the acute 
      infection, the virus may establish a lifelong latency in the neurons of 
      trigeminal ganglia (TG) and other sensory and autonomic ganglia, including the 
      dorsal root ganglia (DRG), etc. The reactivation occurred periodically by a 
      variety of physical or emotional stressors. We have been developing a human DRG 
      neuronal cell-culture model HD10.6, which mimics the mature neurons for latency 
      and reactivation with robust neuronal physiology. We found that miR124 
      overexpression without acyclovir (ACV) could maintain the virus in a quiescent 
      infection, with the accumulation of latency-associate transcript (LAT). The 
      immediate-early (IE) gene ICP0, on the other hand, was very low and the latent 
      viruses could be reactivated by trichostatin A (TSA) treatment. Together, these 
      observations suggested a putative role of microRNA in promoting HSV-1 latency in 
      human neurons.
FAU - Chen, Yu-Chih
AU  - Chen YC
AD  - Department of Pharmaceutical Sciences, School of Pharmacy and Health Professions, 
      University of Maryland Eastern Shore, Princess Anne, MD 21853, USA.
FAU - Li, Hedong
AU  - Li H
AD  - Department of Neuroscience & Regenerative Medicine, Medical College of Georgia, 
      Augusta University, 1120 15th Street, Rm. CA4012, Augusta, GA 30912, USA.
FAU - Martin-Caraballo, Miguel
AU  - Martin-Caraballo M
AUID- ORCID: 0000-0002-1094-4676
AD  - Department of Pharmaceutical Sciences, School of Pharmacy and Health Professions, 
      University of Maryland Eastern Shore, Princess Anne, MD 21853, USA.
FAU - Hsia, Shaochung Victor
AU  - Hsia SV
AUID- ORCID: 0000-0001-7623-2842
AD  - Department of Pharmaceutical Sciences, School of Pharmacy and Health Professions, 
      University of Maryland Eastern Shore, Princess Anne, MD 21853, USA.
LA  - eng
GR  - R01 NS117918/NS/NINDS NIH HHS/United States
GR  - R21 NS104394/NS/NINDS NIH HHS/United States
GR  - R21 NS119732/NS/NINDS NIH HHS/United States
PT  - Journal Article
DEP - 20220716
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC9317301
OTO - NOTNLM
OT  - dorsal root ganglion
OT  - herpesvirus
OT  - host-virus interaction
OT  - latency
OT  - microRNA
OT  - reactivation
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/28 06:01
PMCR- 2022/07/16
CRDT- 2022/07/27 01:37
PHST- 2022/05/16 00:00 [received]
PHST- 2022/06/28 00:00 [revised]
PHST- 2022/07/13 00:00 [accepted]
PHST- 2022/07/27 01:37 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/28 06:01 [medline]
PHST- 2022/07/16 00:00 [pmc-release]
AID - pathogens11070803 [pii]
AID - pathogens-11-00803 [pii]
AID - 10.3390/pathogens11070803 [doi]
PST - epublish
SO  - Pathogens. 2022 Jul 16;11(7):803. doi: 10.3390/pathogens11070803.