PMID- 35891415
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20220804
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 7
DP  - 2022 Jun 29
TI  - Differences in Humoral Immune Response against the Type 2 Porcine Reproductive 
      and Respiratory Syndrome Virus via Different Immune Pathways.
LID - 10.3390/v14071435 [doi]
LID - 1435
AB  - The intramuscular vaccine is the principal strategy to protect pigs from porcine 
      reproductive and respiratory syndrome virus (PRRSV), However, it is still 
      difficult to control PRRSV effectively. This study infected piglets with PRRSV 
      through intramuscular and intranasal inoculation. Subsequently, viral loads, 
      anti-PRRSV antibody levels, and neutralizing antibodies (NAs) titers in both 
      serum and saliva were monitored for 43 days. Meanwhile, tissues were obtained 
      through necropsy at 43 days post-inoculation (dpi) to detect viral loads. The 
      results indicated that viremia lasted from 3 to 31 dpi in both the inoculation 
      groups, but the viruses survived in the lungs and lymph nodes after viremia 
      clearance. The antibody response was detected from 11 dpi, but the response of 
      NAs was delayed until 3-4 weeks. Furthermore, intranasal inoculation induced 
      lower viral load levels than injection inoculation. In addition, positive SIgA 
      and NAs levels were produced early, with higher levels through intranasal 
      inoculation. Therefore, our data indicated that a more robust antibody response 
      and lower virus loads could be induced by intranasal inoculation, and mucosal 
      inoculation could be a suitable pathway for PRRSV vaccines.
FAU - Li, Wen
AU  - Li W
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
FAU - Sun, Yangyang
AU  - Sun Y
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
FAU - Zhao, Shijie
AU  - Zhao S
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
FAU - Cui, Zhiying
AU  - Cui Z
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
FAU - Chen, Yu
AU  - Chen Y
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
FAU - Xu, Pengli
AU  - Xu P
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
FAU - Chen, Jing
AU  - Chen J
AUID- ORCID: 0000-0002-2872-3353
AD  - College of Life Science, Henan Agricultural University, Jinshui District, 
      Zhengzhou 450002, China.
FAU - Zhang, Yina
AU  - Zhang Y
AUID- ORCID: 0000-0003-2993-7270
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
FAU - Xia, Pingan
AU  - Xia P
AD  - College of Veterinary Medicine, Henan Agricultural University, Zhengdong New 
      District Longzi Lake 15#, Zhengzhou 450046, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220629
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing
MH  - Antibodies, Viral
MH  - Immunity, Humoral
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - *Viral Vaccines
MH  - Viremia
PMC - PMC9316826
OTO - NOTNLM
OT  - IgG
OT  - NAs
OT  - PRRSV-2
OT  - SIgA
OT  - in vivo
OT  - mucosal immunity
OT  - viremia
COIS- The authors declare no conflict of interest.
EDAT- 2022/07/28 06:00
MHDA- 2022/07/29 06:00
PMCR- 2022/06/29
CRDT- 2022/07/27 01:44
PHST- 2022/05/05 00:00 [received]
PHST- 2022/06/25 00:00 [revised]
PHST- 2022/06/28 00:00 [accepted]
PHST- 2022/07/27 01:44 [entrez]
PHST- 2022/07/28 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/06/29 00:00 [pmc-release]
AID - v14071435 [pii]
AID - viruses-14-01435 [pii]
AID - 10.3390/v14071435 [doi]
PST - epublish
SO  - Viruses. 2022 Jun 29;14(7):1435. doi: 10.3390/v14071435.