PMID- 35891465 OWN - NLM STAT- MEDLINE DCOM- 20220728 LR - 20220804 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 14 IP - 7 DP - 2022 Jul 7 TI - Inducible miR-150 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication by Targeting Viral Genome and Suppressor of Cytokine Signaling 1. LID - 10.3390/v14071485 [doi] LID - 1485 AB - Hosts exploit various approaches to defend against porcine reproductive and respiratory syndrome virus (PRRSV) infection. microRNAs (miRNAs) have emerged as key negative post-transcriptional regulators of gene expression and have been reported to play important roles in regulating virus infection. Here, we identified that miR-150 was differentially expressed in virus permissive and non-permissive cells. Subsequently, we demonstrated that PRRSV induced the expression of miR-150 via activating the protein kinase C (PKC)/c-Jun amino-terminal kinases (JNK)/c-Jun pathway, and overexpression of miR-150 suppressed PRRSV replication. Further analysis revealed that miR-150 not only directly targeted the PRRSV genome, but also facilitated type I IFN signaling. RNA immunoprecipitation assay demonstrated that miR-150 targeted the suppressor of cytokine signaling 1 (SOCS1), which is a negative regulator of Janus activated kinase (JAK)/signal transducer and activator of the transcription (STAT) signaling pathway. The inverse correlation between miR-150 and SOCS1 expression implies that miR-150 plays a role in regulating ISG expression. In conclusion, miR-150 expression is upregulated upon PRRSV infection. miR-150 feedback positively targets the PRRSV genome and promotes type I IFN signaling, which can be seen as a host defensive strategy. FAU - Li, Sihan AU - Li S AD - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. FAU - Zhang, Xuan AU - Zhang X AD - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. FAU - Yao, Yao AU - Yao Y AD - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. FAU - Zhu, Yingqi AU - Zhu Y AD - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. FAU - Zheng, Xiaojie AU - Zheng X AD - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. FAU - Liu, Fang AU - Liu F AUID- ORCID: 0000-0001-6692-5119 AD - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. FAU - Feng, Wenhai AU - Feng W AD - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. AD - Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220707 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Cytokines) RN - 0 (MicroRNAs) RN - 0 (Suppressor of Cytokine Signaling Proteins) RN - EC 2.7.10.2 (Janus Kinases) SB - IM MH - Animals MH - Cell Line MH - Cytokines/genetics/metabolism MH - Genome, Viral MH - Janus Kinases/metabolism MH - *MicroRNAs/genetics/metabolism MH - *Porcine Reproductive and Respiratory Syndrome/genetics MH - *Porcine respiratory and reproductive syndrome virus/genetics/metabolism MH - Suppressor of Cytokine Signaling Proteins/genetics MH - Swine MH - Virus Replication/physiology PMC - PMC9318191 OTO - NOTNLM OT - HP-PRRSV OT - IFN OT - PKC OT - SOCS1 OT - miR-150 COIS- The authors declare no conflict of interest. EDAT- 2022/07/28 06:00 MHDA- 2022/07/29 06:00 PMCR- 2022/07/07 CRDT- 2022/07/27 01:44 PHST- 2022/06/18 00:00 [received] PHST- 2022/07/04 00:00 [revised] PHST- 2022/07/04 00:00 [accepted] PHST- 2022/07/27 01:44 [entrez] PHST- 2022/07/28 06:00 [pubmed] PHST- 2022/07/29 06:00 [medline] PHST- 2022/07/07 00:00 [pmc-release] AID - v14071485 [pii] AID - viruses-14-01485 [pii] AID - 10.3390/v14071485 [doi] PST - epublish SO - Viruses. 2022 Jul 7;14(7):1485. doi: 10.3390/v14071485.