PMID- 35897733 OWN - NLM STAT- MEDLINE DCOM- 20220729 LR - 20220731 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 23 IP - 15 DP - 2022 Jul 24 TI - Differential MicroRNA Expression in Porcine Endometrium Related to Spontaneous Embryo Loss during Early Pregnancy. LID - 10.3390/ijms23158157 [doi] LID - 8157 AB - Litter size is an important indicator to measure the production capacity of commercial pigs. Spontaneous embryo loss is an essential factor in determining sow litter size. In early pregnancy, spontaneous embryo loss in porcine is as high as 20-30% during embryo implantation. However, the specific molecular mechanism underlying spontaneous embryo loss at the end of embryo implantation remains unknown. Therefore, we comprehensively used small RNA sequencing technology, bioinformatics analysis, and molecular experiments to determine the microRNA (miRNA) expression profile in the healthy and arresting embryo implantation site of porcine endometrium on day of gestation (DG) 28. A total of 464 miRNAs were identified in arresting endometrium (AE) and healthy endometrium (HE), and 139 differentially expressed miRNAs (DEMs) were screened. We combined the mRNA sequencing dataset from the SRA database to predict the target genes of these miRNAs. A quantitative real-time PCR assay identified the expression levels of miRNAs and mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed target genes of DEMs, mainly enriched in epithelial development and amino acids metabolism-related pathways. We performed fluorescence in situ hybridization (FISH) and the dual-luciferase report gene assay to confirm miRNA and predicted target gene binding. miR-205 may inhibit its expression by combining 3'-untranslated regions (3' UTR) of tubulointerstitial nephritis antigen-like 1 (TINAGL1). The resulting inhibition of angiogenesis in the maternal endometrium ultimately leads to the formation of arresting embryos during the implantation period. This study provides a reference for the effect of miRNA on the successful implantation of pig embryos in early gestation. FAU - Gu, Shengchen AU - Gu S AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. FAU - Zang, Xupeng AU - Zang X AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. FAU - Jiang, Lei AU - Jiang L AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. FAU - Gu, Ting AU - Gu T AUID- ORCID: 0000-0002-8732-7377 AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. FAU - Meng, Fanming AU - Meng F AD - State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China. FAU - Huang, Sixiu AU - Huang S AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China. FAU - Cai, Gengyuan AU - Cai G AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China. FAU - Li, Zicong AU - Li Z AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China. AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China. FAU - Wu, Zhenfang AU - Wu Z AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China. AD - State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China. FAU - Hong, Linjun AU - Hong L AUID- ORCID: 0000-0002-6554-7918 AD - National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agri-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China. AD - Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China. LA - eng GR - 31802033/National Natural Science Foundation of China/ GR - 2018143/Guangdong Provincial Promotion Project on Preservation and utilization of Local Breed of Live-stock and Poultry/ PT - Journal Article DEP - 20220724 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (3' Untranslated Regions) RN - 0 (MicroRNAs) SB - IM MH - 3' Untranslated Regions MH - Animals MH - Embryo Implantation/genetics MH - *Embryo Loss/genetics MH - Endometrium/metabolism MH - Female MH - Humans MH - In Situ Hybridization, Fluorescence MH - *MicroRNAs/genetics/metabolism MH - Pregnancy MH - Swine PMC - PMC9331794 OTO - NOTNLM OT - endometrium OT - implantation OT - miRNA OT - pig OT - spontaneous embryo loss COIS- The authors declare no conflict of interest. EDAT- 2022/07/29 06:00 MHDA- 2022/07/30 06:00 PMCR- 2022/07/24 CRDT- 2022/07/28 01:09 PHST- 2022/06/28 00:00 [received] PHST- 2022/07/12 00:00 [revised] PHST- 2022/07/21 00:00 [accepted] PHST- 2022/07/28 01:09 [entrez] PHST- 2022/07/29 06:00 [pubmed] PHST- 2022/07/30 06:00 [medline] PHST- 2022/07/24 00:00 [pmc-release] AID - ijms23158157 [pii] AID - ijms-23-08157 [pii] AID - 10.3390/ijms23158157 [doi] PST - epublish SO - Int J Mol Sci. 2022 Jul 24;23(15):8157. doi: 10.3390/ijms23158157.