PMID- 35901667
OWN - NLM
STAT- MEDLINE
DCOM- 20220816
LR  - 20220816
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1678
DP  - 2022 Aug 16
TI  - Simultaneous quantification of multiple amino acid adducts from sulfur 
      mustard-modified human serum albumin in plasma at trace exposure levels by 
      ultra-high performance liquid chromatography-triple quadrupole mass spectrometry 
      after propionyl derivatization.
PG  - 463354
LID - S0021-9673(22)00547-7 [pii]
LID - 10.1016/j.chroma.2022.463354 [doi]
AB  - Sulfur mustard (HD) is a highly toxic vesicant and is prohibited by the 
      Organisation for the Prohibition of Chemical Weapons (OPCW). HD can modify human 
      serum albumin (HSA) to generate hydroxyethylthioethyl (HETE) adducts, which could 
      be utilized as biomarkers for verifying HD exposure in forensic analysis. Here, 
      five amino acid adducts generated from pronase digestion of HD-exposed human 
      serum albumin (HD-HSA) in plasma were selected as biomarkers to retrospectively 
      detect HD exposure. HD-HSA was precipitated from plasma with acetone, digested by 
      pronase, derivatized with propionic anhydride (PA), and analysed with ultra-high 
      performance liquid chromatography coupled with triple quadrupole mass 
      spectrometry (UHPLC-TQ MS). The limits of detection (LODs) and limits of 
      quantification (LOQs) of the HD exposure concentrations were evaluated as 1.00 
      ng/mL at S/N>/=3 and 3.00 ng/mL at S/N>/=10, respectively, which are approximately 60 
      times lower than those of the reported method. The approach shows good linearity 
      (R(2)>/=0.997) from 3.00 ng/mL to 10.0 microg/mL of HD-exposed human plasma with 
      satisfactory precision and accuracy. The developed approach was applied to 
      analysing samples from the 6(th) OPCW Biomedical Proficiency Test (BioPT). The 
      study showed that the developed approach was also suitable for analysing human 
      plasma samples that were exposed to six of HD analogues, which were common 
      impurities in sulfur mustard mixtures. Moreover, the method was successfully 
      applied to plasma from other species, including rabbits, rats and cattle. This 
      study provides a reliable and sensitive tool for the retrospective detection of 
      vesicants exposure based on multiple biomarkers.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Chen, Bo
AU  - Chen B
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing 102205, P. R. China.
FAU - Ren, Zhe
AU  - Ren Z
AD  - School of Chemistry and Chemical Engineering, Nanjing University of Sciences & 
      Technology, Nanjing, 210094, P. R. China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - State Key Laboratory of Proteomics, Beijing Proteome Research Center, National 
      Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research and 
      Development of New Drug of Chinese Academy of Medical Sciences, Institute of 
      Lifeomics, Beijing, 102206, P. R. China.
FAU - Yu, Huilan
AU  - Yu H
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing 102205, P. R. China.
FAU - Shu, ZhiBin
AU  - Shu Z
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing 102205, P. R. China.
FAU - Liu, Changcai
AU  - Liu C
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing 102205, P. R. China.
FAU - Yang, Yang
AU  - Yang Y
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing 102205, P. R. China.
FAU - Xu, Ping
AU  - Xu P
AD  - State Key Laboratory of Proteomics, Beijing Proteome Research Center, National 
      Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research and 
      Development of New Drug of Chinese Academy of Medical Sciences, Institute of 
      Lifeomics, Beijing, 102206, P. R. China. Electronic address: xuping_bprc@126.com.
FAU - Liu, Shilei
AU  - Liu S
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing 102205, P. R. China. 
      Electronic address: liu_shilei@lacricd.com.
LA  - eng
PT  - Journal Article
DEP - 20220719
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Amino Acids)
RN  - 0 (Biomarkers)
RN  - 0 (Chemical Warfare Agents)
RN  - 0 (Irritants)
RN  - EC 3.4.24.- (Pronase)
RN  - T8KEC9FH9P (Mustard Gas)
RN  - ZIF514RVZR (Serum Albumin, Human)
SB  - IM
MH  - Amino Acids
MH  - Animals
MH  - Biomarkers
MH  - Cattle
MH  - *Chemical Warfare Agents/analysis
MH  - Chromatography, High Pressure Liquid/methods
MH  - Humans
MH  - Irritants/analysis
MH  - *Mustard Gas/analysis
MH  - Pronase/chemistry
MH  - Rabbits
MH  - Rats
MH  - Retrospective Studies
MH  - Serum Albumin, Human/analysis
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Human serum albumin
OT  - Multiple biomarkers
OT  - Retrospective detection
OT  - Sulfur mustard
OT  - Trace exposure
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/07/29 06:00
MHDA- 2022/08/17 06:00
CRDT- 2022/07/28 18:23
PHST- 2022/05/31 00:00 [received]
PHST- 2022/07/17 00:00 [revised]
PHST- 2022/07/18 00:00 [accepted]
PHST- 2022/07/29 06:00 [pubmed]
PHST- 2022/08/17 06:00 [medline]
PHST- 2022/07/28 18:23 [entrez]
AID - S0021-9673(22)00547-7 [pii]
AID - 10.1016/j.chroma.2022.463354 [doi]
PST - ppublish
SO  - J Chromatogr A. 2022 Aug 16;1678:463354. doi: 10.1016/j.chroma.2022.463354. Epub 
      2022 Jul 19.