PMID- 35902902
OWN - NLM
STAT- MEDLINE
DCOM- 20220801
LR  - 20220812
IS  - 2047-9158 (Print)
IS  - 2047-9158 (Electronic)
IS  - 2047-9158 (Linking)
VI  - 11
IP  - 1
DP  - 2022 Jul 28
TI  - Olfactory swab sampling optimization for alpha-synuclein aggregate detection in 
      patients with Parkinson's disease.
PG  - 37
LID - 10.1186/s40035-022-00311-3 [doi]
LID - 37
AB  - BACKGROUND: In patients with Parkinson's disease (PD), real-time quaking-induced 
      conversion (RT-QuIC) detection of pathological alpha-synuclein (alpha-syn) in olfactory 
      mucosa (OM) is not as accurate as in other alpha-synucleinopathies. It is unknown 
      whether these variable results might be related to a different distribution of 
      pathological alpha-syn in OM. Thus, we investigated whether nasal swab (NS) performed 
      in areas with a different coverage by olfactory neuroepithelium, such as agger 
      nasi (AN) and middle turbinate (MT), might affect the detection of pathological 
      alpha-syn. METHODS: NS was performed in 66 patients with PD and 29 non-PD between 
      September 2018 and April 2021. In 43 patients, cerebrospinal fluid (CSF) was also 
      obtained and all samples were analyzed by RT-QuIC for alpha-syn. RESULTS: In the 
      first round, 72 OM samples were collected by NS, from AN (NS(AN)) or from MT 
      (NS(MT)), and 35 resulted positive for alpha-syn RT-QuIC, including 27/32 (84%) from 
      AN, 5/11 (45%) from MT, and 3/29 (10%) belonging to the non-PD patients. 
      Furthermore, 23 additional PD patients underwent NS at both AN and MT, and 
      RT-QuIC revealed alpha-syn positive in 18/23 (78%) NS(AN) samples and in 10/23 (44%) 
      NS(MT) samples. Immunocytochemistry of NS preparations showed a higher 
      representation of olfactory neural cells in NS(AN) compared to NS(MT). We also 
      observed alpha-syn and phospho-alpha-syn deposits in NS from PD patients but not in 
      controls. Finally, RT-QuIC was positive in 22/24 CSF samples from PD patients 
      (92%) and in 1/19 non-PD. CONCLUSION: In PD patients, RT-QuIC sensitivity is 
      significantly increased (from 45% to 84%) when NS is performed at AN, indicating 
      that alpha-syn aggregates are preferentially detected in olfactory areas with higher 
      concentration of olfactory neurons. Although RT-QuIC analysis of CSF showed a 
      higher diagnostic accuracy compared to NS, due to the non-invasiveness, NS might 
      be considered as an ancillary procedure for PD diagnosis.
CI  - (c) 2022. The Author(s).
FAU - Bongianni, Matilde
AU  - Bongianni M
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Catalan, Mauro
AU  - Catalan M
AD  - Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale 
      Cattinara, University of Trieste, 34128, Trieste, Italy.
FAU - Perra, Daniela
AU  - Perra D
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Fontana, Elena
AU  - Fontana E
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Janes, Francesco
AU  - Janes F
AD  - Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
FAU - Bertolotti, Claudio
AU  - Bertolotti C
AD  - Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale 
      Cattinara, University of Trieste, 34128, Trieste, Italy.
FAU - Sacchetto, Luca
AU  - Sacchetto L
AD  - Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University 
      of Verona, 37134, Verona, Italy.
FAU - Capaldi, Stefano
AU  - Capaldi S
AD  - Biocrystallography Laboratory, Department of Biotechnology, University of Verona, 
      37134, Verona, Italy.
FAU - Tagliapietra, Matteo
AU  - Tagliapietra M
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Polverino, Paola
AU  - Polverino P
AD  - Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale 
      Cattinara, University of Trieste, 34128, Trieste, Italy.
FAU - Tommasini, Valentina
AU  - Tommasini V
AD  - Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale 
      Cattinara, University of Trieste, 34128, Trieste, Italy.
FAU - Bellavita, Giulia
AU  - Bellavita G
AD  - Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale 
      Cattinara, University of Trieste, 34128, Trieste, Italy.
FAU - Kachoie, Elham Ataie
AU  - Kachoie EA
AD  - Biocrystallography Laboratory, Department of Biotechnology, University of Verona, 
      37134, Verona, Italy.
FAU - Baruca, Roberto
AU  - Baruca R
AD  - Otolaryngology Unit, Department of Medicine, Surgery and Health Sciences, 
      Ospedale Cattinara, University of Trieste, 34128, Trieste, Italy.
FAU - Bernardini, Andrea
AU  - Bernardini A
AD  - Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
FAU - Valente, Mariarosaria
AU  - Valente M
AD  - Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
FAU - Fiorini, Michele
AU  - Fiorini M
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Bronzato, Erika
AU  - Bronzato E
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Tamburin, Stefano
AU  - Tamburin S
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Bertolasi, Laura
AU  - Bertolasi L
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Brozzetti, Lorenzo
AU  - Brozzetti L
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Cecchini, Maria Paola
AU  - Cecchini MP
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Gigli, Gianluigi
AU  - Gigli G
AD  - Neurology Unit, University of Udine Academic Hospital, 33100, Udine, Italy.
FAU - Monaco, Salvatore
AU  - Monaco S
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy.
FAU - Manganotti, Paolo
AU  - Manganotti P
AD  - Neurology Unit, Department of Medicine, Surgery and Health Sciences, Ospedale 
      Cattinara, University of Trieste, 34128, Trieste, Italy.
FAU - Zanusso, Gianluigi
AU  - Zanusso G
AUID- ORCID: 0000-0001-5199-6264
AD  - Department of Neurosciences, Biomedicine, and Movement Sciences, Policlinico G. 
      B. Rossi, University of Verona, 37134, Verona, Italy. gianluigi.zanusso@univr.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220728
PL  - England
TA  - Transl Neurodegener
JT  - Translational neurodegeneration
JID - 101591861
RN  - 0 (alpha-Synuclein)
SB  - IM
EIN - Transl Neurodegener. 2022 Aug 12;11(1):38. PMID: 35962443
MH  - Humans
MH  - Olfactory Mucosa/chemistry/pathology
MH  - *Parkinson Disease/diagnosis/pathology
MH  - Smell
MH  - *Synucleinopathies
MH  - alpha-Synuclein/cerebrospinal fluid
PMC - PMC9330656
OTO - NOTNLM
OT  - Alpha-synuclein
OT  - Cerebrospinal fluid
OT  - Olfactory mucosa
OT  - Parkinson disease
OT  - Real-time quaking-induced conversion assay
COIS- All authors report no competing interests.
EDAT- 2022/07/29 06:00
MHDA- 2022/08/02 06:00
PMCR- 2022/07/28
CRDT- 2022/07/28 23:50
PHST- 2022/05/20 00:00 [received]
PHST- 2022/07/18 00:00 [accepted]
PHST- 2022/07/28 23:50 [entrez]
PHST- 2022/07/29 06:00 [pubmed]
PHST- 2022/08/02 06:00 [medline]
PHST- 2022/07/28 00:00 [pmc-release]
AID - 10.1186/s40035-022-00311-3 [pii]
AID - 311 [pii]
AID - 10.1186/s40035-022-00311-3 [doi]
PST - epublish
SO  - Transl Neurodegener. 2022 Jul 28;11(1):37. doi: 10.1186/s40035-022-00311-3.