PMID- 35907648
OWN - NLM
STAT- MEDLINE
DCOM- 20220802
LR  - 20220802
IS  - 1875-5364 (Electronic)
IS  - 1875-5364 (Linking)
VI  - 20
IP  - 7
DP  - 2022 Jul
TI  - Jujuboside A inhibits oxidative stress damage and enhances immunomodulatory 
      capacity of human umbilical cord mesenchymal stem cells through up-regulating IDO 
      expression.
PG  - 494-505
LID - S1875-5364(22)60176-6 [pii]
LID - 10.1016/S1875-5364(22)60176-6 [doi]
AB  - Impaired immunomodulatory capacity and oxidative stress are the key factors 
      limiting the effectiveness of mesenchymal stem cell transplantation therapy. The 
      present study was aimed to investigate the effects of jujuboside A (JuA) on the 
      protective effect and immunomodulatory capacity of human umbilical cord 
      mesenchymal stem cells (hUC-MSCs). Hydrogen peroxide was used to establish an 
      oxidative damage model of hUC-MSCs, while PBMCs isolated from rats were used to 
      evaluate the effect of JuA pre-treatment on the immunomodulatory capacity of 
      hUC-MSCs. Furthermore, Hoechst 33258 staining, lactate dehydrogenase test, 
      measurement of malondialdehyde, Western blot, high-performance liquid 
      chromatography; and flow cytometry were performed. Our results indicated that JuA 
      (25 mumol.L(-1)) promoted the proliferation of hUC-MSCs, but did not affect the 
      differentiating capability of these cells. JuA pre-treatment inhibited apoptosis, 
      prevented oxidative damage, and up-regulated the protein expression of nuclear 
      factor-erythroid factor 2-related factor 2 and heme oxygenase 1 in hUC-MSCs in 
      which oxidative stress was induced with H(2)O(2). In addition, JuA pre-treatment 
      enhanced the inhibitory effect of hUC-MSCs against abnormally activated PBMCs, 
      which was related to stimulation of the expression and activity of indoleamine 
      2,3-dioxygenase. In conclusion, our results demonstrate that JuA pre-treatment 
      can enhance the survival and immunomodulatory ability through pathways related to 
      oxidative stress, providing a new option for the improvement of hUC-MSCs in the 
      clinical setting.
CI  - Copyright (c) 2022 China Pharmaceutical University. Published by Elsevier B.V. All 
      rights reserved.
FAU - Chen, Ji-Cong
AU  - Chen JC
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Xiao, Hong-He
AU  - Xiao HH
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Zhang, Qiang
AU  - Zhang Q
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Kong, Liang
AU  - Kong L
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Wang, Tian-Min
AU  - Wang TM
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Tian, Yu
AU  - Tian Y
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Zhao, Yu-Meng
AU  - Zhao YM
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Li, He
AU  - Li H
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Tian, Jin-Ming
AU  - Tian JM
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Wang, Cui
AU  - Wang C
AD  - Department of Neurology, Dalian Municipal Central Hospital Affiliated to Dalian 
      Medical University, Dalian 116033, China. Electronic address: wangc817@163.com.
FAU - Yang, Jing-Xian
AU  - Yang JX
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China. Electronic address: jingxianyang63@126.com.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Chin J Nat Med
JT  - Chinese journal of natural medicines
JID - 101504416
RN  - 0 (Saponins)
RN  - 55466-04-1 (jujuboside A)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - *Mesenchymal Stem Cells
MH  - Oxidative Stress
MH  - Rats
MH  - Saponins
MH  - *Umbilical Cord/metabolism
OTO - NOTNLM
OT  - Human umbilical cord mesenchymal stem cells
OT  - Immunomodulation
OT  - Indoleamine 2,3-dioxygenase
OT  - Jujuboside A
OT  - Oxidative stress
EDAT- 2022/07/31 06:00
MHDA- 2022/08/03 06:00
CRDT- 2022/07/30 21:03
PHST- 2022/02/18 00:00 [received]
PHST- 2022/07/30 21:03 [entrez]
PHST- 2022/07/31 06:00 [pubmed]
PHST- 2022/08/03 06:00 [medline]
AID - S1875-5364(22)60176-6 [pii]
AID - 10.1016/S1875-5364(22)60176-6 [doi]
PST - ppublish
SO  - Chin J Nat Med. 2022 Jul;20(7):494-505. doi: 10.1016/S1875-5364(22)60176-6.