PMID- 35908050
OWN - NLM
STAT- MEDLINE
DCOM- 20220802
LR  - 20221013
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 Jul 30
TI  - Human umbilical cord mesenchymal stem cells derived extracellular vesicles 
      regulate acquired immune response of lupus mouse in vitro.
PG  - 13101
LID - 10.1038/s41598-022-17331-8 [doi]
LID - 13101
AB  - Systemic lupus erythematosus (SLE) is an autoimmune disease involving multiple 
      systems. Immunopathology believes that abnormal T cell function and excessive 
      production of autoantibodies by B cells are involved in multi-organ damage. Human 
      umbilical cord mesenchymal stem cells (hUCMSCs) therapies have endowed with 
      promise in SLE, while the function of MSC-derived extracellular vesicles 
      (MSC-EVs) was still unclear. Extracellular vesicles (EVs) are subcellular 
      components secreted by a paracellular mechanism and are essentially a group of 
      nanoparticles. EVs play a vital role in cell-to-cell communication by acting as 
      biological transporters. New evidence has shown beneficial effects of MSC-EVs on 
      autoimmune diseases, such as their immunomodulatory properties. In this study, we 
      investigated whether hUCMSCs derived extracellular vesicles (hUCMSC-EVs) could 
      regulate abnormal immune responses of T cells or B cells in SLE. We isolated 
      splenic mononuclear cells from MRL/lpr mice, a classical animal model of SLE. PBS 
      (Phosphate-buffered saline), 2 x 10(5) hUCMSCs, 25 microg/ml hUCMSC-EVs, 50 microg/ml 
      hUCMSC-EVs were co-cultured with 2 x 10(6) activated splenic mononuclear cells 
      for 3 days in vitro, respectively. The proportions of CD4(+) T cell subsets, B 
      cells and the concentrations of cytokines were detected. Both hUCMSCs and 
      hUCMSC-EVs inhibited CD4(+) T cells, increased the production of T helper (Th)17 
      cells, promoted the production of interleukin (IL)-17 and transforming growth 
      factor beta1 (TGF-beta1) (P < 0.05), although they had no significant effects on 
      Th1, Th2, T follicular helper (Tfh), regulatory T (Treg) cells and IL-10 
      (P > 0.05); only hUCMSCs inhibited CD19(+) B cells, promoted the production of 
      interferon-gamma (IFN-gamma) and IL-4 (P < 0.05). hUCMSCs exert immunoregulatory 
      effects on SLE at least partially through hUCMSC-EVs in vitro, therefore, 
      hUCMSC-EVs play novel and potential regulator roles in SLE.
CI  - (c) 2022. The Author(s).
FAU - Xie, Min
AU  - Xie M
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Li, Cuifang
AU  - Li C
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - She, Zhou
AU  - She Z
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Wu, Feifeng
AU  - Wu F
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Mao, Jueyi
AU  - Mao J
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Hun, Marady
AU  - Hun M
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Luo, Senlin
AU  - Luo S
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Wan, Wuqing
AU  - Wan W
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Tian, Jidong
AU  - Tian J
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China.
FAU - Wen, Chuan
AU  - Wen C
AD  - Division of Hematology and Tumor, Children's Medical Center, The Second Xiangya 
      Hospital, Central South University, Changsha, Hunan, People's Republic of China. 
      chuanwen@csu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220730
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - *Extracellular Vesicles/metabolism
MH  - Humans
MH  - Immunity
MH  - *Lupus Erythematosus, Systemic
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Mice
MH  - Mice, Inbred MRL lpr
MH  - Umbilical Cord
PMC - PMC9338971
COIS- The authors declare no competing interests.
EDAT- 2022/07/31 06:00
MHDA- 2022/08/03 06:00
PMCR- 2022/07/30
CRDT- 2022/07/30 23:34
PHST- 2022/02/14 00:00 [received]
PHST- 2022/07/25 00:00 [accepted]
PHST- 2022/07/30 23:34 [entrez]
PHST- 2022/07/31 06:00 [pubmed]
PHST- 2022/08/03 06:00 [medline]
PHST- 2022/07/30 00:00 [pmc-release]
AID - 10.1038/s41598-022-17331-8 [pii]
AID - 17331 [pii]
AID - 10.1038/s41598-022-17331-8 [doi]
PST - epublish
SO  - Sci Rep. 2022 Jul 30;12(1):13101. doi: 10.1038/s41598-022-17331-8.