PMID- 35909677
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220802
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Effects of dietary L-leucine supplementation on testicular development and semen 
      quality in boars.
PG  - 904653
LID - 10.3389/fvets.2022.904653 [doi]
LID - 904653
AB  - Sperm and seminal plasma are rich in leucine, and leucine can promote the protein 
      synthesis. This property makes it an interesting amino acid to increase sperm 
      quality of human and livestock spermatogenesis. The goal of this study was to 
      explore the effects of dietary leucine supplementation on testicular development 
      and semen quality in boars from weaning to 10 months of age. 30 pure-bred, weaned 
      Duroc boars (8.0 +/- 1.0 kg) were randomly divided into two groups: control group 
      (CON; fed the basal diet) and leucine group (LEU; fed the basal diet supplemented 
      with 1.2% leucine); then, their body weight and testicular volume were recorded 
      every 4 weeks. Testes were collected for histological and genes expression 
      analysis from 150-day-old boars. Semen was collected and analyzed. Amino acids 
      contents of blood plasma, seminal plasma, sperm, and testes were determined. 
      Dietary supplementation with leucine increased the testicular volume and weight 
      of boars, compared with CON. Sperm viability, sperm count per ejaculation, and 
      average curve speed of sperm in leucine-supplemented boars were increased. 
      Furthermore, leucine supplementation increased the blood plasma and seminal 
      plasma leucine concentrations, and enhanced the gene expressions of branch chain 
      amino acid transaminase, protein kinase B, mammalian target of rapamycin (mTOR), 
      and cyclinb1 in the testes. Interestingly, the expressions of the p-mTOR and mTOR 
      proteins in the testes were also upregulated. Thus, dietary leucine 
      supplementation increased leucine absorption and utilization in the testes, 
      promoted testicular development, and improved semen quality of boars, partly 
      through the mTOR signaling pathway.
CI  - Copyright (c) 2022 Lin, Li, Wang, Fang, Che, Xu, Feng, Zhuo, Li and Wu.
FAU - Lin, Yan
AU  - Lin Y
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Li, Jiayi
AU  - Li J
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Wang, Ke
AU  - Wang K
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Fang, Zhengfeng
AU  - Fang Z
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Che, Lianqiang
AU  - Che L
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Xu, Shengyu
AU  - Xu S
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Feng, Bin
AU  - Feng B
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Zhuo, Yong
AU  - Zhuo Y
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Li, Jian
AU  - Li J
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
FAU - Wu, De
AU  - Wu D
AD  - Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of 
      Agriculture, Animal Nutrition Institute, Sichuan Agricultural University, Yaan, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220715
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9334790
OTO - NOTNLM
OT  - boar
OT  - leucine
OT  - mTOR
OT  - semen quality
OT  - testicular development
EDAT- 2022/08/02 06:00
MHDA- 2022/08/02 06:01
PMCR- 2022/01/01
CRDT- 2022/08/01 03:18
PHST- 2022/03/25 00:00 [received]
PHST- 2022/06/23 00:00 [accepted]
PHST- 2022/08/01 03:18 [entrez]
PHST- 2022/08/02 06:00 [pubmed]
PHST- 2022/08/02 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.904653 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Jul 15;9:904653. doi: 10.3389/fvets.2022.904653. eCollection 
      2022.