PMID- 35911861
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220802
IS  - 2476-4108 (Print)
IS  - 2476-3772 (Electronic)
IS  - 2476-3772 (Linking)
VI  - 20
IP  - 5
DP  - 2022 May
TI  - Relationship between sperm quality and total fertilization failure in 
      intracytoplasmic sperm injection and in vitro fertilization cycles: A 
      cross-sectional study.
PG  - 413-422
LID - 10.18502/ijrm.v20i5.11056 [doi]
AB  - BACKGROUND: Total fertilization failure (TFF) is associated with essential 
      mechanistic and cellular events. OBJECTIVE: The present study is a comprehensive 
      examination of detrimental effects with well-known assays for predicting TFF in 
      conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection 
      (ICSI) cycles. MATERIALS AND METHODS: Semen parameters of 90 men, including 60 
      cases who had experienced IVF/ICSI failure and a control group of 30 individuals, 
      were evaluated. Sperm chromatin/DNA quality assessments were done by aniline 
      blue, toluidine blue, chromomycin A3, and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assays. A lipid hydroperoxide 
      (LPO) kit was used to measure the LPO, and JC1 staining was used to evaluate 
      mitochondrial membrane potential (MMP). RESULTS: There were statistically 
      significant differences found between the IVF, ICSI and control groups by the 
      toluidine blue (p = 0.01), TUNEL (p = 0.02), and chromomycin A3 (p  <  0.001) 
      tests, but not by the aniline blue staining. Furthermore, there was a significant 
      difference regarding LPO concentration and high MMP in cases of IVF fertilization 
      failure compared to the control group (p = 0.04, p = 0.02, respectively). The 
      logistic regression model showed that sperm viability was predictive for 
      fertilization failure in the ICSI group. Sperm chromatin and DNA quality assays 
      were not predictors for TFF in either group. CONCLUSION: Cellular events such as 
      high DNA fragmentation damage, high levels of reactive oxygen species, and low 
      MMP levels can cause TFF in IVF and ICSI programs. Diagnostic tests, especially 
      in cases with previous fertilization failure, showed significant differences in 
      sperm chromatin and DNA quality between groups but could not predict the risk of 
      TFF.
CI  - Copyright (c) 2022 Safari et al.
FAU - Safari, Hassan
AU  - Safari H
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AD  - Department of Reproductive Biology, Shahid Sadoughi University of Medical 
      Sciences, Yazd, Iran.
FAU - Anbari, Fatemeh
AU  - Anbari F
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AD  - Department of Reproductive Biology, Shahid Sadoughi University of Medical 
      Sciences, Yazd, Iran.
FAU - Ghasemi-Esmailabad, Saeed
AU  - Ghasemi-Esmailabad S
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
FAU - Maleki, Behnam
AU  - Maleki B
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AD  - Department of Reproductive Biology, Shahid Sadoughi University of Medical 
      Sciences, Yazd, Iran.
FAU - Dehghan Marvast, Laleh
AU  - Dehghan Marvast L
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AD  - Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi 
      University of Medical Sciences, Yazd, Iran.
FAU - Reza Talebi, Ali
AU  - Reza Talebi A
AUID- ORCID: 0000-0003-4883-3020
AD  - Research and Clinical Center for Infertility, Yazd Reproductive Sciences 
      Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
AD  - Andrology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi 
      University of Medical Sciences, Yazd, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220608
PL  - Iran
TA  - Int J Reprod Biomed
JT  - International journal of reproductive biomedicine
JID - 101679102
PMC - PMC9334897
OTO - NOTNLM
OT  - Chromatin
OT  - DNA fragmentation.
OT  - Reactive oxygen species
OT  - In vitro fertilization
OT  - Intracytoplasmic sperm injection
COIS- The authors declare that there is no conflict of interest.
EDAT- 2022/08/02 06:00
MHDA- 2022/08/02 06:01
PMCR- 2022/06/08
CRDT- 2022/08/01 03:53
PHST- 2021/08/04 00:00 [received]
PHST- 2021/09/11 00:00 [revised]
PHST- 2021/12/06 00:00 [accepted]
PHST- 2022/08/01 03:53 [entrez]
PHST- 2022/08/02 06:00 [pubmed]
PHST- 2022/08/02 06:01 [medline]
PHST- 2022/06/08 00:00 [pmc-release]
AID - 10.18502/ijrm.v20i5.11056 [doi]
PST - epublish
SO  - Int J Reprod Biomed. 2022 Jun 8;20(5):413-422. doi: 10.18502/ijrm.v20i5.11056. 
      eCollection 2022 May.