PMID- 35912075
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220802
IS  - 1663-4365 (Print)
IS  - 1663-4365 (Electronic)
IS  - 1663-4365 (Linking)
VI  - 14
DP  - 2022
TI  - Huangqin Decoction Exerts Beneficial Effects on Rotenone-Induced Rat Model of 
      Parkinson's Disease by Improving Mitochondrial Dysfunction and Alleviating 
      Metabolic Abnormality of Mitochondria.
PG  - 911924
LID - 10.3389/fnagi.2022.911924 [doi]
LID - 911924
AB  - Parkinson's disease (PD) is a common neurodegenerative disease, and the 
      pathogenesis of PD is closely related to mitochondrial dysfunction. Previous 
      studies have indicated that traditional Chinese medicine composition of Huangqin 
      Decoction (HQD), including Scutellariae Radix, licorice, and Paeoniae Radix Alba, 
      has therapeutic effects on PD, but whether HQD has a therapeutic effect on PD has 
      not been reported. In this study, the protective effects of HQD on 
      rotenone-induced PD rats were evaluated by behavioral assays (open field, 
      rotating rod, suspension, gait, inclined plate, and grid) and 
      immunohistochemistry. The mechanisms of HQD on attenuation of mitochondrial 
      dysfunction were detected by biochemical assays and mitochondrial metabolomics. 
      The results showed that HQD (20 g/kg) can protect rats with PD by improving motor 
      coordination and muscle strength, increasing the number of tyrosine hydroxylase 
      (TH)-positive neurons in rats with PD. Besides, HQD can improve mitochondrial 
      dysfunction by increasing the content of adenosine triphosphate (ATP) and 
      mitochondrial complex I. Mitochondrial metabolomics analysis revealed that the 
      ketone body of acetoacetic acid (AcAc) in the rotenone group was significantly 
      higher than that of the control group. Ketone bodies have been known to be used 
      as an alternative energy source to provide energy to the brain when glucose was 
      deficient. Further studies demonstrated that HQD could increase the expression of 
      glucose transporter GLUT1, the content of tricarboxylic acid cycle rate-limiting 
      enzyme citrate synthase (CS), and the level of hexokinase (HK) in rats with PD 
      but could decrease the content of ketone bodies [AcAc and beta-hydroxybutyric acid 
      (beta-HB)] and the expression of their transporters (MCT1). Our study revealed that 
      the decrease of glucose metabolism in the rotenone group was parallel to the 
      increase of substitute substrates (ketone bodies) and related transporters, and 
      HQD could improve PD symptoms by activating the aerobic glycolysis pathway.
CI  - Copyright (c) 2022 Gao, Cao, Du and Qin.
FAU - Gao, Li
AU  - Gao L
AD  - Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of 
      Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi 
      University, Taiyuan, China.
AD  - Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi 
      Province, Taiyuan, China.
FAU - Cao, Min
AU  - Cao M
AD  - Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of 
      Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi 
      University, Taiyuan, China.
AD  - Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi 
      Province, Taiyuan, China.
FAU - Du, Guan-Hua
AU  - Du GH
AD  - Peking Union Medical College, Institute of Materia Medica, Chinese Academy of 
      Medical Sciences, Beijing, China.
FAU - Qin, Xue-Mei
AU  - Qin XM
AD  - Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of 
      Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi 
      University, Taiyuan, China.
AD  - Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi 
      Province, Taiyuan, China.
LA  - eng
PT  - Journal Article
DEP - 20220715
PL  - Switzerland
TA  - Front Aging Neurosci
JT  - Frontiers in aging neuroscience
JID - 101525824
PMC - PMC9334858
OTO - NOTNLM
OT  - Huangqin Decoction
OT  - Parkinson's disease
OT  - aerobic glycolysis
OT  - ketone bodies
OT  - mitochondria
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/02 06:00
MHDA- 2022/08/02 06:01
PMCR- 2022/01/01
CRDT- 2022/08/01 03:56
PHST- 2022/04/03 00:00 [received]
PHST- 2022/06/20 00:00 [accepted]
PHST- 2022/08/01 03:56 [entrez]
PHST- 2022/08/02 06:00 [pubmed]
PHST- 2022/08/02 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnagi.2022.911924 [doi]
PST - epublish
SO  - Front Aging Neurosci. 2022 Jul 15;14:911924. doi: 10.3389/fnagi.2022.911924. 
      eCollection 2022.