PMID- 35913918 OWN - NLM STAT- MEDLINE DCOM- 20220803 LR - 20220826 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 17 IP - 8 DP - 2022 TI - Experimental study on tailings cementation by MICP technique with immersion curing. PG - e0272281 LID - 10.1371/journal.pone.0272281 [doi] LID - e0272281 AB - The filling mining method is an effective method for controlling ground stress and preventing surface subsidence in the mining field during exploitation of underground resources. Tailings can be utilized as the filling material, so as to realize the reuse of industrial waste. However, utilization of the traditional Portland cement as the cementing material for tailings leads to groundwater pollution. In addition, production of Portland cement results in consumption of a great amount of ore and air pollution. In this paper, a tailings cementation method by using the microbial induced calcite precipitation (MICP) technique with immersion curing is proposed. Tailings are cemented by the MICP technique with aerobic bacteria (Sporosarcina pasteurii) under a soaked curing environment. The variable control method is applied to investigate the factors influencing the cementation effects by the MICP technique with Sporosarcina pasteurii, including the bacterial solution concentration, the cementing solution concentration, the particle size of tailings, and the curing temperature. The results indicate that: when OD600 of the Sporosarcina pasteurii solution is 1.6, the urea concentration in the cementing solution is 0.75 mol/L, the tailings are raw materials without grinding, and the curing temperature is 30 degrees C, the cementation effect is the best. In view of uneven calcification during MICP with Sporosarcina pasteurii, mixed Sporosarcina pasteurii and Castellaniella denitrificans are used for tailings cementation. Higher strength of cemented tailings is achieved. It is proved that the MICP technique with mixed aerobic bacteria and facultative anaerobes is an effective method for tailings cementation. FAU - Jin, Changyu AU - Jin C AUID- ORCID: 0000-0001-5723-6282 AD - Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines, Northeastern University, Shenyang, Liaoning, 110004, China. FAU - Liu, Huiyang AU - Liu H AD - Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines, Northeastern University, Shenyang, Liaoning, 110004, China. FAU - Guo, Mingxiao AU - Guo M AD - Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines, Northeastern University, Shenyang, Liaoning, 110004, China. FAU - Wang, Yunfeng AU - Wang Y AD - Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines, Northeastern University, Shenyang, Liaoning, 110004, China. FAU - Zhu, Jinyao AU - Zhu J AD - Key Laboratory of Ministry of Education on Safe Mining of Deep Metal Mines, Northeastern University, Shenyang, Liaoning, 110004, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220801 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - H0G9379FGK (Calcium Carbonate) RN - Sporosarcina pasteurii SB - IM MH - Bacteria MH - *Calcium Carbonate MH - Cementation MH - Immersion MH - *Sporosarcina PMC - PMC9342764 COIS- The authors have declared that no competing interests exist. EDAT- 2022/08/02 06:00 MHDA- 2022/08/04 06:00 PMCR- 2022/08/01 CRDT- 2022/08/01 13:33 PHST- 2021/12/27 00:00 [received] PHST- 2022/07/16 00:00 [accepted] PHST- 2022/08/01 13:33 [entrez] PHST- 2022/08/02 06:00 [pubmed] PHST- 2022/08/04 06:00 [medline] PHST- 2022/08/01 00:00 [pmc-release] AID - PONE-D-21-40689 [pii] AID - 10.1371/journal.pone.0272281 [doi] PST - epublish SO - PLoS One. 2022 Aug 1;17(8):e0272281. doi: 10.1371/journal.pone.0272281. eCollection 2022.