PMID- 35914717 OWN - NLM STAT- MEDLINE DCOM- 20220908 LR - 20221018 IS - 1876-7737 (Electronic) IS - 1874-3919 (Linking) VI - 267 DP - 2022 Sep 15 TI - TMT-based quantitative membrane proteomics identified PRRs potentially involved in the perception of MSP1 in rice leaves. PG - 104687 LID - S1874-3919(22)00211-1 [pii] LID - 10.1016/j.jprot.2022.104687 [doi] AB - Pathogen-associated molecular patterns (PAMPs) play a key role in triggering PAMPs triggered immunity (PTI) in plants. In the case of the rice-Magnaporthe oryzae pathosystem, fewer PAMPs and their pattern recognition receptors (PRRs) have been characterized. Recently, a M. oryzae snodprot1 homolog protein (MSP1) has been identified that functions as PAMP and triggering the PTI responses in rice. However, the molecular mechanism underlying MSP1-induced PTI is currently elusive. Therefore, we generated MSP1 overexpressed transgenic lines of rice, and a tandem mass tag (TMT)-based quantitative membrane proteomic analysis was employed to decipher the potential MSP1-induced signaling in rice using total cytosolic as well as membrane protein fractions. This approach led to the identification of 8033 proteins of which 1826 were differentially modulated in response to overexpression of MSP1 and/or exogenous jasmonic acid treatment. Of these, 20 plasma membrane-localized receptor-like kinases (RLKs) showed increased abundance in MSP1 overexpression lines. Moreover, activation of proteins related to the protein degradation and modification, calcium signaling, redox, and MAPK signaling was observed in transgenic lines expressing MSP1 in the apoplast. Taken together, our results identified potential PRR candidates involved in MSP1 recognition and suggested the overview mechanism of the MSP1-induced PTI signaling in rice leaves. SIGNIFICANCE: In plants, recognition of pathogen pathogen-derived molecules, such as PAMPs, by plant plant-derived PRRs has an essential role for in the activation of PTI against pathogen invasion. Typically, PAMPs are recognized by plasma membrane (PM) localized PRRs, however, identifying the PM-localized PRR proteins is challenging due to their low abundance. In this study, we performed an integrated membrane protein enrichment by microsomal membrane extraction (MME) method and subsequent TMT-labeling-based quantitative proteomic analysis using MSP1 overexpressed rice. Based on these results, we successfully identified various intracellular and membrane membrane-localized proteins that participated in the MSP1-induced immune response and characterized the potential PM-localized PRR candidates in rice. CI - Copyright (c) 2022 Elsevier B.V. All rights reserved. FAU - Min, Cheol Woo AU - Min CW AD - Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. FAU - Jang, Jeong Woo AU - Jang JW AD - Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. FAU - Lee, Gi Hyun AU - Lee GH AD - Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. FAU - Gupta, Ravi AU - Gupta R AD - College of General Education, Kookmin University, Seoul 02707, Republic of Korea. FAU - Yoon, Jinmi AU - Yoon J AD - Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. FAU - Park, Hyun Ji AU - Park HJ AD - Plant System Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea. FAU - Cho, Hye Sun AU - Cho HS AD - Plant System Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea. FAU - Park, Sang Ryeol AU - Park SR AD - National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of Korea. FAU - Kwon, Soon-Wook AU - Kwon SW AD - Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. FAU - Cho, Lae-Hyeon AU - Cho LH AD - Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. FAU - Jung, Ki-Hong AU - Jung KH AD - Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin 17104, Republic of Korea. FAU - Kim, Yu-Jin AU - Kim YJ AD - Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. FAU - Wang, Yiming AU - Wang Y AD - Key Laboratory of Biological Interactions and Crop Health, Department of Plant Pathology, Nanjing Agricultural University, 210095, Nanjing, China. FAU - Kim, Sun Tae AU - Kim ST AD - Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea. Electronic address: stkim71@pusan.ac.kr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220730 PL - Netherlands TA - J Proteomics JT - Journal of proteomics JID - 101475056 RN - 0 (Merozoite Surface Protein 1) RN - 0 (Pathogen-Associated Molecular Pattern Molecules) RN - 0 (Receptors, Pattern Recognition) SB - IM MH - Merozoite Surface Protein 1/metabolism MH - *Oryza/metabolism MH - Pathogen-Associated Molecular Pattern Molecules MH - Perception MH - Plant Diseases MH - Plant Leaves/metabolism MH - Plants/metabolism MH - Proteomics MH - Receptors, Pattern Recognition/metabolism OTO - NOTNLM OT - MSP1 OT - Magnaporthe oryzae OT - PRRs OT - Proteomics OT - Rice OT - TMT EDAT- 2022/08/02 06:00 MHDA- 2022/09/09 06:00 CRDT- 2022/08/01 19:15 PHST- 2022/05/07 00:00 [received] PHST- 2022/07/05 00:00 [revised] PHST- 2022/07/17 00:00 [accepted] PHST- 2022/08/02 06:00 [pubmed] PHST- 2022/09/09 06:00 [medline] PHST- 2022/08/01 19:15 [entrez] AID - S1874-3919(22)00211-1 [pii] AID - 10.1016/j.jprot.2022.104687 [doi] PST - ppublish SO - J Proteomics. 2022 Sep 15;267:104687. doi: 10.1016/j.jprot.2022.104687. Epub 2022 Jul 30.