PMID- 35917592 OWN - NLM STAT- MEDLINE DCOM- 20221101 LR - 20221101 IS - 1532-2661 (Electronic) IS - 0034-5288 (Linking) VI - 152 DP - 2022 Dec 20 TI - Cardiac gene activation varies between young and adult cats and in the presence of hypertrophic cardiomyopathy. PG - 38-47 LID - S0034-5288(22)00210-7 [pii] LID - 10.1016/j.rvsc.2022.07.007 [doi] AB - Little is known about the difference of myocardial gene transcription in young and adult cats and how transcription is further modified in cats with hypertrophic cardiomyopathy (HCM) and with left atrial (LA) thrombus formation. We hypothesized that selected factors for coagulation, endothelial activation, inflammation, and remodelling are modified with age and are activated in the hearts of cats with HCM. Left atrial and ventricular (LV) samples from 12 cats with HCM (seven without (HCMwoAT] and five with LA thrombi [HCMwAT]), and six young (YC) and six adult (AC) control cats without cardiac disease were investigated for relative expression of the following genes using quantitative polymerase chain reaction: von Willebrand factor, a disintegrin and metalloproteinase with a thrombospondin type 1 motif member 13, platelet activating factor, E- and P-selectin, intercellular and vascular adhesion molecules-1, ss2-integrin, vascular endothelial growth factor, interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), heat shock protein-70, and myocyte-specific enhancer factor 2C. Significant differences in gene activation were found between YC and AC, and YC and cats with HCM. Compared to AC, MCP-1 and IL-6 were significantly higher in cats with HCM. The presence of an LA thrombus was associated with higher IL-6 expression. These results illustrate the relevance of age and/or lifestyle on gene expression in the feline heart. The gene transcription pattern found in AC hearts might predispose cats to their characteristic cardiac remodelling processes and thrombus formation if disease occurs. It further supports the involvement of inflammation, but not coagulation and endothelial activation, in HCM. CI - Copyright (c) 2022 Elsevier Ltd. All rights reserved. FAU - Colpitts, Michelle E AU - Colpitts ME AD - Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada. Electronic address: m.e.colpitts@gmail.com. FAU - Caswell, Jeff L AU - Caswell JL AD - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada. Electronic address: jcaswell@uoguelph.ca. FAU - Monteith, Gabrielle AU - Monteith G AD - Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada. Electronic address: gmonteit@uoguelph.ca. FAU - Joshua, Jessica AU - Joshua J AD - Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada; Department of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada. Electronic address: jjoshua@uoguelph.ca. FAU - O'Sullivan, M Lynne AU - O'Sullivan ML AD - Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada. Electronic address: mosullivan@upei.ca. FAU - Raheb, Shari AU - Raheb S AD - Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada. Electronic address: raheb@uoguelph.ca. FAU - Fonfara, Sonja AU - Fonfara S AD - Department of Clinical Studies, Ontario Veterinary College, University of Guelph, 50 Stone Road E., Guelph, ON N1G 2W1, Canada. Electronic address: sfonfara@uoguelph.ca. LA - eng PT - Journal Article DEP - 20220723 PL - England TA - Res Vet Sci JT - Research in veterinary science JID - 0401300 RN - 0 (Interleukin-6) RN - 0 (Vascular Endothelial Growth Factor A) SB - IM MH - Cats MH - Animals MH - Transcriptional Activation MH - Interleukin-6/genetics MH - Vascular Endothelial Growth Factor A MH - *Cardiomyopathy, Hypertrophic/genetics/veterinary MH - *Thrombosis/veterinary MH - Inflammation/veterinary MH - *Cat Diseases/genetics OTO - NOTNLM OT - Age OT - Feline OT - Heart OT - Inflammation OT - Remodelling OT - thrombus EDAT- 2022/08/03 06:00 MHDA- 2022/11/02 06:00 CRDT- 2022/08/02 18:12 PHST- 2021/09/13 00:00 [received] PHST- 2022/06/23 00:00 [revised] PHST- 2022/07/15 00:00 [accepted] PHST- 2022/08/03 06:00 [pubmed] PHST- 2022/11/02 06:00 [medline] PHST- 2022/08/02 18:12 [entrez] AID - S0034-5288(22)00210-7 [pii] AID - 10.1016/j.rvsc.2022.07.007 [doi] PST - ppublish SO - Res Vet Sci. 2022 Dec 20;152:38-47. doi: 10.1016/j.rvsc.2022.07.007. Epub 2022 Jul 23.