PMID- 35917673
OWN - NLM
STAT- MEDLINE
DCOM- 20220829
LR  - 20220829
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 101
IP  - 9
DP  - 2022 Sep
TI  - Comparison of the performance of targeted mitochondrial antioxidant mitoquinone 
      and non-targeted antioxidant pentoxifylline in improving rooster sperm parameters 
      during freezing and thawing.
PG  - 102035
LID - S0032-5791(22)00326-1 [pii]
LID - 10.1016/j.psj.2022.102035 [doi]
LID - 102035
AB  - Oxidative stress is associated with impaired sperm quality after thawing. Since 
      mitochondria are the main source of reactive oxygen species (ROS) in sperm, the 
      aim of this study was to investigate the effects of targeted mitochondrial 
      antioxidant mitoquinone (MitoQ) and non-targeted mitochondrial antioxidant 
      pentoxifylline (PTX) during cooling and cryopreservation of rooster sperm. Sperm 
      samples were collected from 15 roosters aged 28 wk and diluted with Beltsville 
      extender. After dilution and addition of treatments (50, 100, and 200 pMol MitoQ 
      and 0.5, 0.75, and 1 muM PTX), samples were cooled for 2 h to 4 degrees C and they were 
      first analyzed at this stage and were frozen and re-evaluated after thawing. 
      After the freezing and thawing, level of 100 pMol MitoQ significantly increased 
      total motility (TM), progressive motility (PGM), curvilinear velocity (VCL), 
      membrane integrity, viability, total antioxidant capacity (TAC) and the 
      glutathione peroxidase (GPx), as well as the level of 50 pMol significantly 
      increased TM, PGM, average path velocity (VAP), straight-line velocity (VSL), 
      membrane integrity, viability, and mitochondrial activity. Moreover, these 2 
      levels (50 and 100 PMol) decreased malondialdehyde and sperm with abnormal 
      morphology. Addition of 0.75 muM PTX also increased total motility compared to the 
      control group and levels of 0.5 and 0.75 muM decreased sperm with abnormal 
      morphology. It could be concluded the addition of MitoQ and PTX can be useful for 
      sperm cryopreservation industry and reduce the harmful effects of freeze-thawing.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Nazari, Mahdi
AU  - Nazari M
AD  - Department of Animal Science, College of Agriculture, University of Tabriz, 
      Tabriz, Iran.
FAU - Daghigh-Kia, Hossein
AU  - Daghigh-Kia H
AD  - Department of Animal Science, College of Agriculture, University of Tabriz, 
      Tabriz, Iran.
FAU - Mehdipour, Mahdieh
AU  - Mehdipour M
AD  - Department of Animal Science, College of Agriculture, University of Tabriz, 
      Tabriz, Iran.
FAU - Najafi, Abouzar
AU  - Najafi A
AD  - Department of Animal and Poultry Science, College of Aburaihan, University of 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220628
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antioxidants)
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Organophosphorus Compounds)
RN  - 1339-63-5 (Ubiquinone)
RN  - 47BYS17IY0 (mitoquinone)
RN  - SD6QCT3TSU (Pentoxifylline)
SB  - IM
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Chickens
MH  - Cryopreservation/veterinary
MH  - Cryoprotective Agents/pharmacology
MH  - Freezing
MH  - Male
MH  - Mitochondria
MH  - Organophosphorus Compounds
MH  - *Pentoxifylline/metabolism/pharmacology
MH  - Semen
MH  - Semen Analysis/veterinary
MH  - *Semen Preservation/veterinary
MH  - Sperm Motility
MH  - Spermatozoa
MH  - Ubiquinone/analogs & derivatives
PMC - PMC9343413
OTO - NOTNLM
OT  - mitochondria activity
OT  - mitoquinone
OT  - pentoxifylline
OT  - sperm
COIS- DISCLOSURES None of the authors have any conflict of interest to declare.
EDAT- 2022/08/03 06:00
MHDA- 2022/08/30 06:00
PMCR- 2022/06/28
CRDT- 2022/08/02 18:20
PHST- 2022/01/10 00:00 [received]
PHST- 2022/06/22 00:00 [revised]
PHST- 2022/06/22 00:00 [accepted]
PHST- 2022/08/03 06:00 [pubmed]
PHST- 2022/08/30 06:00 [medline]
PHST- 2022/08/02 18:20 [entrez]
PHST- 2022/06/28 00:00 [pmc-release]
AID - S0032-5791(22)00326-1 [pii]
AID - 102035 [pii]
AID - 10.1016/j.psj.2022.102035 [doi]
PST - ppublish
SO  - Poult Sci. 2022 Sep;101(9):102035. doi: 10.1016/j.psj.2022.102035. Epub 2022 Jun 
      28.