PMID- 35920787
OWN - NLM
STAT- MEDLINE
DCOM- 20221031
LR  - 20230804
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 34
IP  - 11
DP  - 2022 Oct 27
TI  - The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate 
      alternative splicing to repress photomorphogenesis in Arabidopsis.
PG  - 4191-4212
LID - 10.1093/plcell/koac235 [doi]
AB  - Light is a key environmental signal that regulates plant growth and development. 
      While posttranscriptional regulatory mechanisms of gene expression include 
      alternative splicing (AS) of pre-messenger RNA (mRNA) in both plants and animals, 
      how light signaling affects AS in plants is largely unknown. Here, we identify 
      DExD/H RNA helicase U2AF65-associated protein (UAP56) as a negative regulator of 
      photomorphogenesis in Arabidopsis thaliana. UAP56 is encoded by the homologs 
      UAP56a and UAP56b. Knockdown of UAP56 led to enhanced photomorphogenic responses 
      and diverse developmental defects during vegetative and reproductive growth. 
      UAP56 physically interacts with the central light signaling repressor 
      constitutive photomorphogenic 1 (COP1) and U2AF65. Global transcriptome analysis 
      revealed that UAP56 and COP1 co-regulate the transcription of a subset of genes. 
      Furthermore, deep RNA-sequencing analysis showed that UAP56 and COP1 control 
      pre-mRNA AS in both overlapping and distinct manners. Ribonucleic acid 
      immunoprecipitation assays showed that UAP56 and COP1 bind to common small 
      nuclear RNAs and mRNAs of downstream targets. Our study reveals that both UAP56 
      and COP1 function as splicing factors that coordinately regulate AS during 
      light-regulated plant growth and development.
CI  - (c) American Society of Plant Biologists 2022. All rights reserved. For 
      permissions, please email: journals.permissions@oup.com.
FAU - Li, Yang
AU  - Li Y
AUID- ORCID: 0000-0003-0243-7268
AD  - Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 
      Beijing 100093, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
FAU - Du, Yanxin
AU  - Du Y
AUID- ORCID: 0000-0003-0950-1218
AD  - Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 
      Beijing 100093, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
FAU - Huai, Junling
AU  - Huai J
AUID- ORCID: 0000-0003-0278-5641
AD  - Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 
      Beijing 100093, China.
FAU - Jing, Yanjun
AU  - Jing Y
AUID- ORCID: 0000-0002-4772-7923
AD  - Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 
      Beijing 100093, China.
FAU - Lin, Rongcheng
AU  - Lin R
AUID- ORCID: 0000-0001-8346-3390
AD  - Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 
      Beijing 100093, China.
AD  - University of Chinese Academy of Sciences, Beijing 100049, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - 0 (Arabidopsis Proteins)
RN  - EC 3.6.4.13 (RNA Helicases)
RN  - EC 3.6.1.- (UAP56 protein, Arabidopsis)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Animals
MH  - *Arabidopsis/metabolism
MH  - Ubiquitin-Protein Ligases/genetics/metabolism
MH  - *Arabidopsis Proteins/genetics/metabolism
MH  - Gene Expression Regulation, Plant/genetics
MH  - Alternative Splicing/genetics
MH  - RNA Helicases/genetics
MH  - Light
MH  - DEAD-box RNA Helicases/genetics/metabolism
PMC - PMC9614450
EDAT- 2022/08/04 06:00
MHDA- 2022/11/01 06:00
PMCR- 2023/08/03
CRDT- 2022/08/03 10:43
PHST- 2022/02/28 00:00 [received]
PHST- 2022/07/12 00:00 [accepted]
PHST- 2022/08/04 06:00 [pubmed]
PHST- 2022/11/01 06:00 [medline]
PHST- 2022/08/03 10:43 [entrez]
PHST- 2023/08/03 00:00 [pmc-release]
AID - 6654597 [pii]
AID - koac235 [pii]
AID - 10.1093/plcell/koac235 [doi]
PST - ppublish
SO  - Plant Cell. 2022 Oct 27;34(11):4191-4212. doi: 10.1093/plcell/koac235.