PMID- 35922705
OWN - NLM
STAT- MEDLINE
DCOM- 20221122
LR  - 20221122
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 187
IP  - 5-6
DP  - 2022 Dec
TI  - The Impact of Paracoccidioides spp Infection on Central Nervous System Cell 
      Junctional Complexes.
PG  - 567-577
LID - 10.1007/s11046-022-00653-6 [doi]
AB  - Paracoccidioidomycosis (PCM), a systemic mycosis caused by the fungus 
      Paracoccidioides spp. is the most prevalent fungal infection among 
      immunocompetent patients in Latin America. The estimated frequency of central 
      nervous system (CNS) involvement among the human immunodeficiency virus 
      (HIV)/PCM-positive population is 2.5%. We aimed to address the impact of 
      neuroparacoccidioidomycosis (NPCM) and HIV/NPCM co-infection on the tight 
      junctions (TJ) and adherens junction (AJ) proteins of the CNS. Four CNS 
      formalin-fixed paraffin-embedded (FFPE) tissue specimens were studied: NPCM, 
      NPCM/HIV co-infection, HIV-positive without opportunistic CNS infection, and 
      normal brain autopsy (negative control). Immunohistochemistry was used to analyze 
      the endothelial cells and astrocytes expressions of TJ markers: claudins 
      (CLDN)-1, -3, -5 and occludin; AJ markers: beta-catenin and E-cadherin; and pericyte 
      marker: alpha-smooth muscle actin. FFPE CNS tissue specimens were analyzed using 
      the immunoperoxidase assay. CLDN-5 expression in the capillaries of the HIV/NPCM 
      coinfected tissues (mixed clinical form of PCM) was lower than that in the 
      capillaries of the HIV or NPCM monoinfected (chronic clinical form of PCM) 
      tissues. A marked decrease in CLDN-5 expression and a compensatory increase in 
      CLDN-1 expression in the NPCM/HIV co-infection tissue samples was observed. The 
      authors suggest that Paracoccidioides spp. crosses the blood-brain barrier 
      through paracellular pathway, owing to the alteration in the CLDN expression, or 
      inside the macrophages (Trojan horse).
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - de Almeida, Sergio Monteiro
AU  - de Almeida SM
AUID- ORCID: 0000-0001-5690-105X
AD  - Medical Pathology Department, School of Medicine, Universidade Federal do Parana, 
      Curitiba, Parana, Brazil. sergio.ma@ufpr.br.
AD  - Neuroinfection Outclinic, Hospital de Clinicas, Universidade Federal do Parana, 
      Rua Padre Camargo 280, Curitiba, Parana, 80060-240, Brazil. sergio.ma@ufpr.br.
FAU - Kulik, Amanda
AU  - Kulik A
AD  - Medical Pathology Department, School of Medicine, Universidade Federal do Parana, 
      Curitiba, Parana, Brazil.
FAU - Malaquias, Mineia Alessandra Scaranello
AU  - Malaquias MAS
AD  - Laboratorio de Patologia Experimental, Escola de Medicina- Pontificia 
      Universidade Catolica do Parana, Curitiba, Parana, Brazil.
FAU - Nagashima, Seigo
AU  - Nagashima S
AD  - Laboratorio de Patologia Experimental, Escola de Medicina- Pontificia 
      Universidade Catolica do Parana, Curitiba, Parana, Brazil.
FAU - de Paula, Caroline Busatta Vaz
AU  - de Paula CBV
AD  - Laboratorio de Patologia Experimental, Escola de Medicina- Pontificia 
      Universidade Catolica do Parana, Curitiba, Parana, Brazil.
FAU - Muro, Marisol Dominguez
AU  - Muro MD
AD  - Micology Laboratory, Hospital de Clinicas, Universidade Federal do Parana, 
      Curitiba, Parana, Brazil.
FAU - de Noronha, Lucia
AU  - de Noronha L
AD  - Medical Pathology Department, School of Medicine, Universidade Federal do Parana, 
      Curitiba, Parana, Brazil.
AD  - Laboratorio de Patologia Experimental, Escola de Medicina- Pontificia 
      Universidade Catolica do Parana, Curitiba, Parana, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20220803
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
SB  - IM
MH  - Humans
MH  - *Paracoccidioidomycosis/microbiology
MH  - *Paracoccidioides
MH  - *Coinfection
MH  - Endothelial Cells
MH  - *Central Nervous System Fungal Infections/microbiology
MH  - Central Nervous System
MH  - *HIV Infections/complications
OTO - NOTNLM
OT  - Adherens junction
OT  - Central nervous system
OT  - HIV
OT  - Paracoccidioides spp.
OT  - Spinal cord
OT  - Tight junctions
EDAT- 2022/08/04 06:00
MHDA- 2022/11/23 06:00
CRDT- 2022/08/03 23:33
PHST- 2022/03/07 00:00 [received]
PHST- 2022/07/12 00:00 [accepted]
PHST- 2022/08/04 06:00 [pubmed]
PHST- 2022/11/23 06:00 [medline]
PHST- 2022/08/03 23:33 [entrez]
AID - 10.1007/s11046-022-00653-6 [pii]
AID - 10.1007/s11046-022-00653-6 [doi]
PST - ppublish
SO  - Mycopathologia. 2022 Dec;187(5-6):567-577. doi: 10.1007/s11046-022-00653-6. Epub 
      2022 Aug 3.