PMID- 35924399 OWN - NLM STAT- MEDLINE DCOM- 20220901 LR - 20220906 IS - 2042-650X (Electronic) IS - 2042-6496 (Linking) VI - 13 IP - 17 DP - 2022 Aug 30 TI - Dietary emulsifier glycerol monodecanoate affects the gut microbiota contributing to regulating lipid metabolism, insulin sensitivity and inflammation. PG - 8804-8817 LID - 10.1039/d2fo01689c [doi] AB - Glycerol monodecanoate (GMD) is a medium-chain monoacylglycerol that possesses emulsifying and antibacterial properties. The common emulsifiers carboxymethylcellulose and polysorbate-80 have been reported to cause intestinal microbiota dysbiosis and metabolic disturbances. Glycerol monolaurate (GML), another medium-chain monoacylglycerol, is often used as an emulsifier and could improve metabolism by regulating the gut microbiota. However, research on the effects of GMD on the metabolism and gut microbiota remains scarce. Mice were fed a normal chow diet with or without GMD (150, 800, and 1600 mg kg(-1)) for 22 weeks. Metabolism indicators and related genes, gut microbiota, and fecal SCFAs were analyzed. The results demonstrated that GMD significantly improved insulin sensitivity, reduced the serum LPS level, and decreased pro-inflammation cytokines including IL-1beta, tumor necrosis factor (TNF), and monocyte chemotactic protein 1 (MCP-1). Additionally, 150 and 1600 mg kg(-1) GMD could significantly lower the blood glucose content. 1600 mg kg(-1) GMD improved cholesterol metabolism and related gene expression compared to 150 and 800 mg kg(-1) GMD. Moreover, 150 and 800 mg kg(-1) GMD up-regulated the abundance of Lactobacillus and Turicibacter, while 1600 mg kg(-1) GMD significantly up-regulated the abundance of Bifidobacterium. Our findings indicated that different doses of GMD had inconsistent effects on lipid metabolism by differentially altering the gut microbiota composition. Meanwhile, all doses of GMD showed excellent effects on increasing insulin sensitivity and improving inflammation. FAU - Zhang, Junhui AU - Zhang J AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China. AD - Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058, China. FAU - Yu, Huilin AU - Yu H AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China. AD - Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058, China. FAU - Zhong, Hao AU - Zhong H AUID- ORCID: 0000-0003-2344-861X AD - College of Food Science and Technology, Zhejiang University of Technology, Hangzhou, 310014, China. FAU - Wang, Qianqian AU - Wang Q AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China. AD - Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058, China. FAU - Tang, Jun AU - Tang J AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - School of Life Sciences, Westlake University, Hangzhou, 310005, China. FAU - Shen, Fei AU - Shen F AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China. AD - Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058, China. FAU - Cai, Haiying AU - Cai H AD - School of Biological & Chemical Engineering, Zhejiang University of Science &Technology, Hangzhou, 310023, China. FAU - Liu, Tao AU - Liu T AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China. AD - Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058, China. FAU - Feng, Fengqin AU - Feng F AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China. AD - Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058, China. FAU - Zhao, Minjie AU - Zhao M AUID- ORCID: 0000-0002-3599-2682 AD - College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China. minjiezhao@zju.edu.cn. AD - National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang University, Hangzhou, 310058, China. AD - Key Laboratory for Agro-Products Nutritional Evaluation of Ministry of Agriculture, Zhejiang University, Hangzhou, 310058, China. LA - eng PT - Journal Article DEP - 20220830 PL - England TA - Food Funct JT - Food & function JID - 101549033 RN - 0 (Emulsifying Agents) RN - 0 (Monoglycerides) RN - PDC6A3C0OX (Glycerol) SB - IM MH - Animals MH - Diet MH - Diet, High-Fat MH - Dysbiosis MH - Emulsifying Agents MH - *Gastrointestinal Microbiome MH - Glycerol MH - Inflammation/drug therapy/microbiology MH - *Insulin Resistance MH - Lipid Metabolism MH - Mice MH - Mice, Inbred C57BL MH - Monoglycerides/pharmacology EDAT- 2022/08/05 06:00 MHDA- 2022/09/02 06:00 CRDT- 2022/08/04 03:44 PHST- 2022/08/05 06:00 [pubmed] PHST- 2022/09/02 06:00 [medline] PHST- 2022/08/04 03:44 [entrez] AID - 10.1039/d2fo01689c [doi] PST - epublish SO - Food Funct. 2022 Aug 30;13(17):8804-8817. doi: 10.1039/d2fo01689c.