PMID- 35927233
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220808
IS  - 2058-7716 (Print)
IS  - 2058-7716 (Electronic)
IS  - 2058-7716 (Linking)
VI  - 8
IP  - 1
DP  - 2022 Aug 4
TI  - Circular RNA circPOSTN promotes neovascularization by regulating 
      miR-219a-2-3p/STC1 axis and stimulating the secretion of VEGFA in glioblastoma.
PG  - 349
LID - 10.1038/s41420-022-01136-9 [doi]
LID - 349
AB  - Glioblastoma (GBM), the most malignant type of astrocytic tumor, is one of the 
      deadliest cancers prevalent in adults. Along with tumor growth, patients with GBM 
      generally suffer from extensive cerebral edema and apparent symptoms of 
      intracranial hyper-pressure. Accumulating evidence has demonstrated that circRNA 
      plays a critically important role in tumorigenesis and progression. However, the 
      biological function and the underlying mechanism of circRNA in GBM remain 
      elusive. In this study, by conducting gene expression detection based on 15 pairs 
      of GBM clinical specimens and the normal adjunct tissues, we observed that 
      circPOSTN showed abnormally higher expression in GBM. Both loss-of-function and 
      gain-of-function biological experiments demonstrated that circPOSTN scheduled the 
      proliferation, migration, and neovascularization abilities of GBM cells. Further, 
      fluorescence in situ hybridization (FISH) assay, quantitative RT-PCR, and 
      subcellular separation suggested that circPOSTN was predominately localized in 
      the cytoplasm and may serve as a competing endogenous RNA (ceRNA). CircRNA-miRNA 
      interaction prediction based on online analytical processing, AGO2-RIP assay, 
      biotin labeled RNA pulldown assay, and dual-luciferase reporter assay revealed 
      that circPOSTN sponged miR-219a-2-3p, limited its biological function, and 
      ultimately upregulated their common downstream gene STC1. Finally, by carrying 
      out in vitro and in vivo functional assays, we uncovered a new regulatory axis 
      circPOSTN/miR-219a-2-3p/STC1 that promoted GBM neovascularization by increasing 
      vascular endothelial growth factor A (VEGFA) secretion. Our study underscores the 
      critical role of circPOSTN in GBM progression, providing a novel insight into GBM 
      anti-tumor therapy.
CI  - (c) 2022. The Author(s).
FAU - Long, Niya
AU  - Long N
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China.
FAU - Xu, Xu
AU  - Xu X
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China.
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China.
FAU - Lin, Hongyi
AU  - Lin H
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China.
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China.
FAU - Lv, Ying
AU  - Lv Y
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China.
FAU - Zou, Shenghui
AU  - Zou S
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China.
FAU - Cao, Han
AU  - Cao H
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China.
FAU - Chen, Xueshu
AU  - Chen X
AD  - Department of Clinical Laboratory, Guizhou Cancer Hospital, Guiyang, Guizhou, 
      China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education & Key 
      Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical 
      University, Guiyang, Guizhou, China.
FAU - Qi, Xiaolan
AU  - Qi X
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China.
AD  - Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education & Key 
      Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical 
      University, Guiyang, Guizhou, China.
FAU - Yang, Hua
AU  - Yang H
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China.
FAU - Liu, Jian
AU  - Liu J
AD  - Department of Neurosurgery, Guizhou Provincial People's Hospital, Guiyang, 
      Guizhou, China.
FAU - Chu, Liangzhao
AU  - Chu L
AUID- ORCID: 0000-0002-1861-2473
AD  - Department of Neurosurgery, the Affiliated Hospital of Guizhou Medical 
      University, Guiyang, Guizhou, China. chulz_gmu@163.com.
AD  - School of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou, China. 
      chulz_gmu@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220804
PL  - United States
TA  - Cell Death Discov
JT  - Cell death discovery
JID - 101665035
PMC - PMC9352789
COIS- The authors declare no competing interests.
EDAT- 2022/08/05 06:00
MHDA- 2022/08/05 06:01
PMCR- 2022/08/04
CRDT- 2022/08/04 23:15
PHST- 2021/11/08 00:00 [received]
PHST- 2022/07/18 00:00 [accepted]
PHST- 2022/07/13 00:00 [revised]
PHST- 2022/08/04 23:15 [entrez]
PHST- 2022/08/05 06:00 [pubmed]
PHST- 2022/08/05 06:01 [medline]
PHST- 2022/08/04 00:00 [pmc-release]
AID - 10.1038/s41420-022-01136-9 [pii]
AID - 1136 [pii]
AID - 10.1038/s41420-022-01136-9 [doi]
PST - epublish
SO  - Cell Death Discov. 2022 Aug 4;8(1):349. doi: 10.1038/s41420-022-01136-9.