PMID- 35929265
OWN - NLM
STAT- MEDLINE
DCOM- 20220808
LR  - 20220808
IS  - 2772-9508 (Electronic)
IS  - 2772-9508 (Linking)
VI  - 137
DP  - 2022 Jun
TI  - Effect of high content nanohydroxyapatite composite scaffolds prepared via melt 
      extrusion additive manufacturing on the osteogenic differentiation of human 
      mesenchymal stromal cells.
PG  - 212833
LID - S2772-9508(22)00110-8 [pii]
LID - 10.1016/j.bioadv.2022.212833 [doi]
AB  - The field of bone tissue engineering seeks to mimic the bone extracellular matrix 
      composition, balancing the organic and inorganic components. In this regard, 
      additive manufacturing (AM) of high content calcium phosphate (CaP)-polymer 
      composites holds great promise towards the design of bioactive scaffolds. Yet, 
      the biological performance of such scaffolds is still poorly characterized. In 
      this study, melt extrusion AM (ME-AM) was used to fabricate poly(ethylene oxide 
      terephthalate)/poly(butylene terephthalate) (PEOT/PBT)-nanohydroxyapatite (nHA) 
      scaffolds with up to 45 wt% nHA, which presented significantly enhanced 
      compressive mechanical properties, to evaluate their in vitro osteogenic 
      potential as a function of nHA content. While osteogenic gene upregulation and 
      matrix mineralization were observed on all scaffold types when cultured in 
      osteogenic media, human mesenchymal stromal cells did not present an explicitly 
      clear osteogenic phenotype, within the evaluated timeframe, in basic media 
      cultures (i.e. without osteogenic factors). Yet, due to the adsorption of calcium 
      and inorganic phosphate ions from cell culture media and simulated body fluid, 
      the formation of a CaP layer was observed on PEOT/PBT-nHA 45 wt% scaffolds, which 
      is hypothesized to account for their bone forming ability in the long term in 
      vitro, and osteoconductivity in vivo.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Camara-Torres, Maria
AU  - Camara-Torres M
AD  - Maastricht University, MERLN Institute for Technology-Inspired Regenerative 
      Medicine, Complex Tissue Regeneration Department, Universiteitssingel 40, 6229 ER 
      Maastricht, the Netherlands.
FAU - Sinha, Ravi
AU  - Sinha R
AD  - Maastricht University, MERLN Institute for Technology-Inspired Regenerative 
      Medicine, Complex Tissue Regeneration Department, Universiteitssingel 40, 6229 ER 
      Maastricht, the Netherlands.
FAU - Sanchez, Alberto
AU  - Sanchez A
AD  - TECNALIA, Basque Research and Technology Alliance (BRTA), 20009 Donostia-San 
      Sebastian, Spain.
FAU - Habibovic, Pamela
AU  - Habibovic P
AD  - Maastricht University, MERLN Institute for Technology-Inspired Regenerative 
      Medicine, Instructive Biomaterial Engineering Department, Universiteitssingel 40, 
      6229 ER Maastricht, the Netherlands.
FAU - Patelli, Alessandro
AU  - Patelli A
AD  - Department of Physics and Astronomy, Padova University, Via Marzolo, 8, 35131 
      Padova, Italy.
FAU - Mota, Carlos
AU  - Mota C
AD  - Maastricht University, MERLN Institute for Technology-Inspired Regenerative 
      Medicine, Complex Tissue Regeneration Department, Universiteitssingel 40, 6229 ER 
      Maastricht, the Netherlands.
FAU - Moroni, Lorenzo
AU  - Moroni L
AD  - Maastricht University, MERLN Institute for Technology-Inspired Regenerative 
      Medicine, Complex Tissue Regeneration Department, Universiteitssingel 40, 6229 ER 
      Maastricht, the Netherlands. Electronic address: 
      l.moroni@maastrichtuniversity.nl.
LA  - eng
PT  - Journal Article
DEP - 20220501
PL  - Netherlands
TA  - Biomater Adv
JT  - Biomaterials advances
JID - 9918383886206676
SB  - IM
MH  - Bone Regeneration
MH  - Cell Differentiation
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - *Osteogenesis
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - Bone regeneration
OT  - Composite
OT  - Human mesenchymal stromal cells
OT  - Melt extrusion additive manufacturing
OT  - Nanohydroxyapatite
OT  - Osteogenic differentiation
EDAT- 2022/08/06 06:00
MHDA- 2022/08/09 06:00
CRDT- 2022/08/05 03:44
PHST- 2022/03/01 00:00 [received]
PHST- 2022/04/12 00:00 [revised]
PHST- 2022/04/27 00:00 [accepted]
PHST- 2022/08/05 03:44 [entrez]
PHST- 2022/08/06 06:00 [pubmed]
PHST- 2022/08/09 06:00 [medline]
AID - S2772-9508(22)00110-8 [pii]
AID - 10.1016/j.bioadv.2022.212833 [doi]
PST - ppublish
SO  - Biomater Adv. 2022 Jun;137:212833. doi: 10.1016/j.bioadv.2022.212833. Epub 2022 
      May 1.