PMID- 35929607
OWN - NLM
STAT- MEDLINE
DCOM- 20220808
LR  - 20231105
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 11
DP  - 2022 Aug 5
TI  - Septin7 is indispensable for proper skeletal muscle architecture and function.
LID - 10.7554/eLife.75863 [doi]
LID - e75863
AB  - Today septins are considered as the fourth component of the cytoskeleton, with 
      the Septin7 isoform playing a critical role in the formation of higher-order 
      structures. While its importance has already been confirmed in several 
      intracellular processes of different organs, very little is known about its role 
      in skeletal muscle. Here, using Septin7 conditional knockdown (KD) mouse model, 
      the C2C12 cell line, and enzymatically isolated adult muscle fibers, the 
      organization and localization of septin filaments are revealed, and an 
      ontogenesis-dependent expression of Septin7 is demonstrated. KD mice displayed a 
      characteristic hunchback phenotype with skeletal deformities, reduction in in 
      vivo and in vitro force generation, and disorganized mitochondrial networks. 
      Furthermore, knockout of Septin7 in C2C12 cells resulted in complete loss of cell 
      division while KD cells provided evidence that Septin7 is essential for proper 
      myotube differentiation. These and the transient increase in Septin7 expression 
      following muscle injury suggest that it may be involved in muscle regeneration 
      and development.
CI  - (c) 2022, Gonczi et al.
FAU - Gonczi, Monika
AU  - Gonczi M
AUID- ORCID: 0000-0002-8421-4369
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
FAU - Raduly, Zsolt
AU  - Raduly Z
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
AD  - Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary.
FAU - Szabo, Laszlo
AU  - Szabo L
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
AD  - Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary.
FAU - Fodor, Janos
AU  - Fodor J
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
FAU - Telek, Andrea
AU  - Telek A
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
FAU - Dobrosi, Nora
AU  - Dobrosi N
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
FAU - Balogh, Norbert
AU  - Balogh N
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
AD  - Doctoral School of Molecular Medicine, University of Debrecen, Debrecen, Hungary.
FAU - Szentesi, Peter
AU  - Szentesi P
AUID- ORCID: 0000-0003-2621-2282
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
FAU - Kis, Greta
AU  - Kis G
AD  - Department of Anatomy, Histology and Embryology, Faculty of Medicine, University 
      of Debrecen, Debrecen, Hungary.
FAU - Antal, Miklos
AU  - Antal M
AUID- ORCID: 0000-0002-2457-7387
AD  - Department of Anatomy, Histology and Embryology, Faculty of Medicine, University 
      of Debrecen, Debrecen, Hungary.
FAU - Trencsenyi, Gyorgy
AU  - Trencsenyi G
AD  - Division of Nuclear Medicine and Translational Imaging, Department of Medical 
      Imaging, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
FAU - Dienes, Beatrix
AU  - Dienes B
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
FAU - Csernoch, Laszlo
AU  - Csernoch L
AUID- ORCID: 0000-0002-2446-1456
AD  - Department of Physiology, Faculty of Medicine, University of Debrecen, Debrecen, 
      Hungary.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220805
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
RN  - EC 3.6.1.- (Sept7 protein, mouse)
RN  - EC 3.6.1.- (Septins)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Mice
MH  - Mitochondria/metabolism
MH  - *Muscle Fibers, Skeletal/metabolism
MH  - *Muscle, Skeletal/metabolism
MH  - Septins/genetics/metabolism
PMC - PMC9355566
OTO - NOTNLM
OT  - cell biology
OT  - cytoskeleton
OT  - development
OT  - human
OT  - mitochondria
OT  - mouse
OT  - regeneration
OT  - septins
OT  - skeletal muscle
COIS- MG, ZR, LS, JF, AT, ND, NB, PS, GK, MA, GT, BD, LC No competing interests 
      declared
EDAT- 2022/08/06 06:00
MHDA- 2022/08/09 06:00
PMCR- 2022/08/05
CRDT- 2022/08/05 07:53
PHST- 2021/11/25 00:00 [received]
PHST- 2022/07/03 00:00 [accepted]
PHST- 2022/08/05 07:53 [entrez]
PHST- 2022/08/06 06:00 [pubmed]
PHST- 2022/08/09 06:00 [medline]
PHST- 2022/08/05 00:00 [pmc-release]
AID - 75863 [pii]
AID - 10.7554/eLife.75863 [doi]
PST - epublish
SO  - Elife. 2022 Aug 5;11:e75863. doi: 10.7554/eLife.75863.