PMID- 35930970
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220930
IS  - 1879-033X (Electronic)
IS  - 0959-440X (Linking)
VI  - 75
DP  - 2022 Aug
TI  - Structural basis of mRNA maturation: Time to put it together.
PG  - 102431
LID - S0959-440X(22)00110-5 [pii]
LID - 10.1016/j.sbi.2022.102431 [doi]
AB  - In eukaryotes, the expression of genetic information begins in the cell nucleus 
      with precursor messenger RNA (pre-mRNA) transcription and processing into mature 
      mRNA. The mRNA is subsequently recognized and packaged by proteins into an mRNA 
      ribonucleoprotein complex (mRNP) and exported to the cytoplasm for translation. 
      Each of the nuclear mRNA maturation steps is carried out by a dedicated molecular 
      machine. Here, we highlight recent structural and mechanistic insights into how 
      these machines function, including the capping enzyme, the spliceosome, the 
      3'-end processing machinery, and the transcription-export complex. While we 
      increasingly understand individual steps of nuclear gene expression, many 
      questions remain. For example, we are only beginning to reveal how mature mRNAs 
      are recognized and packaged for nuclear export and how mRNA maturation events are 
      coupled to transcription and to each other. Advances in the preparation of 
      recombinant and endogenous protein-nucleic acid complexes, cryo-electron 
      microscopy, and machine learning promise exciting insights into the mechanisms of 
      nuclear gene expression and its spatial organization.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Vorlander, Matthias K
AU  - Vorlander MK
AD  - Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), 
      Campus-Vienna-Biocenter 1, 1030, Vienna, Austria. Electronic address: 
      https://twitter.com/@MVorlandr.
FAU - Pacheco-Fiallos, Belen
AU  - Pacheco-Fiallos B
AD  - Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), 
      Campus-Vienna-Biocenter 1, 1030, Vienna, Austria; Vienna BioCenter PhD Program, 
      Doctoral School of the University of Vienna and Medical University of Vienna, 
      1030, Vienna, Austria. Electronic address: https://twitter.com/@bpachecofiallos.
FAU - Plaschka, Clemens
AU  - Plaschka C
AD  - Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), 
      Campus-Vienna-Biocenter 1, 1030, Vienna, Austria. Electronic address: 
      clemens.plaschka@imp.ac.at.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20220802
PL  - England
TA  - Curr Opin Struct Biol
JT  - Current opinion in structural biology
JID - 9107784
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Active Transport, Cell Nucleus
MH  - *Cell Nucleus/metabolism
MH  - Cryoelectron Microscopy
MH  - RNA Precursors/metabolism
MH  - *RNA Transport
MH  - RNA, Messenger/genetics/metabolism
COIS- Conflict of interest None declared.
EDAT- 2022/08/06 06:00
MHDA- 2022/08/24 06:00
CRDT- 2022/08/05 18:27
PHST- 2022/04/23 00:00 [received]
PHST- 2022/06/02 00:00 [revised]
PHST- 2022/06/14 00:00 [accepted]
PHST- 2022/08/06 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/08/05 18:27 [entrez]
AID - S0959-440X(22)00110-5 [pii]
AID - 10.1016/j.sbi.2022.102431 [doi]
PST - ppublish
SO  - Curr Opin Struct Biol. 2022 Aug;75:102431. doi: 10.1016/j.sbi.2022.102431. Epub 
      2022 Aug 2.