PMID- 35932505
OWN - NLM
STAT- MEDLINE
DCOM- 20220830
LR  - 20221006
IS  - 1980-5322 (Electronic)
IS  - 1807-5932 (Print)
IS  - 1807-5932 (Linking)
VI  - 77
DP  - 2022
TI  - Resveratrol attenuates chronic pulmonary embolism-related endothelial cell injury 
      by modulating oxidative stress, inflammation, and autophagy.
PG  - 100083
LID - S1807-5932(22)03284-7 [pii]
LID - 10.1016/j.clinsp.2022.100083 [doi]
LID - 100083
AB  - OBJECTIVES: Due to Pulmonary Artery Endothelial Cell (PAEC) dysfunction, 
      Pulmonary Hypertension (PH) persists even after the Pulmonary Embolism (PE) has 
      been relieved. However, the mechanism behind this remains unclear. METHOD: Here, 
      the authors incubated Human PAECs (HPAECs) with thrombin to simulate the process 
      of arterial thrombosis. RESULTS: CCK8 results showed a decrease in the viability 
      of HPAECs after thrombin incubation. In addition, the expression of Tissue Factor 
      (TF), Monocyte Chemoattractant Protein 1 (MCP-1), VCAM-1, ICAM-1, cleaved 
      caspase 3, cleaved caspase 9, and Bax protein were all increased after thrombin 
      incubation, while Bcl-2 was decreased. The effects of 3-MA treatment further 
      suggested that autophagy might mediate the partial protective effects of 
      Resveratrol on HPAECs. To observe the effects of Resveratrol in vivo, the authors 
      established a Chronic Thromboembolic Pulmonary Hypertension (CTEPH) model by 
      repeatedly injecting autologous blood clots into a rat's left jugular vein. The 
      results exhibited that Mean Pulmonary Arterial Pressure (mPAP) and vessel Wall 
      Area/Total Area (WA/TA) ratio were both decreased after Resveratrol treatment. 
      Moreover, Resveratrol could reduce the concentration and activity of TF, vWF, 
      P-selectin, and promote these Superoxide Dismutase (SOD) in plasma. Western blot 
      analysis of inflammation, platelet activation, autophagy, and 
      apoptosis-associated proteins in pulmonary artery tissue validated the results in 
      PHAECs. CONCLUSIONS: These findings suggested that reduced autophagy, increased 
      oxidative stress, increased platelet activation, and increased inflammation were 
      involved in CTEPH-induced HPAEC dysfunction and the development of PH, while 
      Resveratrol could improve PAEC dysfunction and PH.
CI  - Copyright (c) 2022. Published by Elsevier Espana, S.L.U.
FAU - Liu, Xiaopeng
AU  - Liu X
AD  - Department of Respiratory Medicine, Jinshan Hospital Affiliated to Fudan 
      University, Shanghai, China.
FAU - Zhou, Haiying
AU  - Zhou H
AD  - Department of Respiratory Medicine, Jinshan Hospital Affiliated to Fudan 
      University, Shanghai, China.
FAU - Hu, Zhixiong
AU  - Hu Z
AD  - Department of Respiratory Medicine, Jinshan Hospital Affiliated to Fudan 
      University, Shanghai, China. Electronic address: huzx321@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20220803
PL  - United States
TA  - Clinics (Sao Paulo)
JT  - Clinics (Sao Paulo, Brazil)
JID - 101244734
RN  - EC 3.4.21.5 (Thrombin)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - Endothelial Cells
MH  - Humans
MH  - *Hypertension, Pulmonary
MH  - Inflammation
MH  - Oxidative Stress
MH  - Pulmonary Artery
MH  - *Pulmonary Embolism
MH  - Rats
MH  - Resveratrol
MH  - Thrombin
PMC - PMC9357834
OTO - NOTNLM
OT  - Autophagy
OT  - Chronic thromboembolic pulmonary hypertension
OT  - Inflammation
OT  - Oxidative stress
OT  - Pulmonary artery endothelial dysfunction
OT  - Resveratrol
COIS- Conflicts of interest The authors declare no conflicts of interest.
EDAT- 2022/08/07 06:00
MHDA- 2022/08/31 06:00
PMCR- 2022/08/03
CRDT- 2022/08/06 18:14
PHST- 2021/12/16 00:00 [received]
PHST- 2022/05/04 00:00 [accepted]
PHST- 2022/08/07 06:00 [pubmed]
PHST- 2022/08/31 06:00 [medline]
PHST- 2022/08/06 18:14 [entrez]
PHST- 2022/08/03 00:00 [pmc-release]
AID - S1807-5932(22)03284-7 [pii]
AID - 100083 [pii]
AID - 10.1016/j.clinsp.2022.100083 [doi]
PST - epublish
SO  - Clinics (Sao Paulo). 2022 Aug 3;77:100083. doi: 10.1016/j.clinsp.2022.100083. 
      eCollection 2022.