PMID- 35933595
OWN - NLM
STAT- MEDLINE
DCOM- 20221020
LR  - 20230127
IS  - 2192-2659 (Electronic)
IS  - 2192-2640 (Linking)
VI  - 11
IP  - 20
DP  - 2022 Oct
TI  - Living Biomaterials to Engineer Hematopoietic Stem Cell Niches.
PG  - e2200964
LID - 10.1002/adhm.202200964 [doi]
AB  - Living biointerfaces are a new class of biomaterials combining living cells and 
      polymeric matrices that can act as biologically active and instructive materials 
      that host and provide signals to surrounding cells. Here, living biomaterials 
      based on Lactococcus lactis to control hematopoietic stem cells in 2D surfaces 
      and 3D hydrogels are introduced. L. lactis is modified to express C-X-C motif 
      chemokine ligand 12 (CXCL12), thrombopoietin (TPO), vascular cell adhesion 
      protein 1 (VCAM1), and the 7th-10th type III domains of human plasma fibronectin 
      (FN III(7-10) ), in an attempt to mimic ex vivo the conditions of the human bone 
      marrow. These results suggest that living biomaterials that incorporate bacteria 
      expressing recombinant CXCL12, TPO, VCAM1, and FN in both 2D systems direct 
      hematopoietic stem and progenitor cells (HSPCs)-bacteria interaction, and in 3D 
      using hydrogels functionalized with full-length human plasma fibronectin allow 
      for a notable expansion of the CD34(+) /CD38(-) /CD90(+) HSPC population compared 
      to the initial population. These results provide a strong evidence based on data 
      that suggest the possibility of using living materials based on genetically 
      engineered bacteria for the ex-vivo expansion of HSPC with eventual practical 
      clinical applications in HSPCs transplantation for hematological disorders.
CI  - (c) 2022 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.
FAU - Petaroudi, Michaela
AU  - Petaroudi M
AD  - Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, G12 
      8LT, UK.
FAU - Rodrigo-Navarro, Aleixandre
AU  - Rodrigo-Navarro A
AD  - Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, G12 
      8LT, UK.
FAU - Dobre, Oana
AU  - Dobre O
AD  - Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, G12 
      8LT, UK.
FAU - Dalby, Matthew J
AU  - Dalby MJ
AD  - Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, G12 
      8LT, UK.
FAU - Salmeron-Sanchez, Manuel
AU  - Salmeron-Sanchez M
AUID- ORCID: 0000-0002-8112-2100
AD  - Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, G12 
      8LT, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220818
PL  - Germany
TA  - Adv Healthc Mater
JT  - Advanced healthcare materials
JID - 101581613
RN  - 0 (Fibronectins)
RN  - 9014-42-0 (Thrombopoietin)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Ligands)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Humans
MH  - *Fibronectins/metabolism
MH  - *Thrombopoietin/metabolism
MH  - Biocompatible Materials/metabolism
MH  - Ligands
MH  - Hematopoietic Stem Cells
MH  - Hydrogels/metabolism
OTO - NOTNLM
OT  - cell engineering
OT  - genetic engineering
OT  - hematopoiesis
OT  - living materials
OT  - microenvironment engineering
OT  - stem cells
OT  - synthetic biology
EDAT- 2022/08/08 06:00
MHDA- 2022/10/21 06:00
CRDT- 2022/08/07 03:52
PHST- 2022/08/01 00:00 [revised]
PHST- 2022/04/27 00:00 [received]
PHST- 2022/08/08 06:00 [pubmed]
PHST- 2022/10/21 06:00 [medline]
PHST- 2022/08/07 03:52 [entrez]
AID - 10.1002/adhm.202200964 [doi]
PST - ppublish
SO  - Adv Healthc Mater. 2022 Oct;11(20):e2200964. doi: 10.1002/adhm.202200964. Epub 
      2022 Aug 18.