PMID- 35933832
OWN - NLM
STAT- MEDLINE
DCOM- 20220829
LR  - 20221003
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 574
DP  - 2022 Sep
TI  - Identification of CD163 regions that are required for porcine reproductive and 
      respiratory syndrome virus (PRRSV) infection but not for binding to viral 
      envelope glycoproteins.
PG  - 71-83
LID - S0042-6822(22)00123-4 [pii]
LID - 10.1016/j.virol.2022.07.012 [doi]
AB  - CD163, a receptor for porcine reproductive and respiratory syndrome virus 
      (PRRSV), possesses nine scavenger receptor cysteine-rich (SRCR) and two 
      proline-serine-threonine (PST) domains. To identify CD163 regions involved in 
      PRRSV infection, CD163 mutants were generated. Infection experiments showed 
      resistance to infection following deletion of the SRCR4/5 interdomain or the Exon 
      13 that encodes a portion of PSTII. The mutation of a pentapeptide domain in 
      SRCR5 and SRCR7 also conferred resistance. Mutant CD163 proteins that resisted 
      infection retained the ability to interact with GP2, GP3, GP4 and GP5 viral 
      glycoproteins. The contribution of multiple domains to infection but not to the 
      binding of viral glycoproteins suggests that the envelope proteins may form 
      multiple interactions with CD163, or that receptor regions important for 
      infection have other cellular binding partners required for PRRSV infection. 
      Finally, we mapped the localization the anti-CD163 2A10 antibody epitope.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Stoian, Ana M M
AU  - Stoian AMM
AD  - School of Medicine, Department of Medial Microbiology and Immunology, University 
      of California Davis, Davis, CA, USA.
FAU - Rowland, Raymond R R
AU  - Rowland RRR
AD  - Department of Pathobiology, College of Veterinary Medicine Administration, 
      University of Illinois at Urbana-Champaign, Champaign, IL, USA.
FAU - Brandariz-Nunez, Alberto
AU  - Brandariz-Nunez A
AD  - Department of Pathobiology, College of Veterinary Medicine Administration, 
      University of Illinois at Urbana-Champaign, Champaign, IL, USA. Electronic 
      address: Brandari@illinois.edu.
LA  - eng
PT  - Journal Article
DEP - 20220731
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (Mutant Proteins)
RN  - 0 (Receptors, Scavenger)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation, Myelomonocytic/genetics/metabolism
MH  - Mutant Proteins
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus/genetics/metabolism
MH  - Receptors, Scavenger
MH  - Swine
MH  - Viral Envelope Proteins/genetics
EDAT- 2022/08/08 06:00
MHDA- 2022/08/30 06:00
CRDT- 2022/08/07 18:11
PHST- 2022/05/23 00:00 [received]
PHST- 2022/07/21 00:00 [revised]
PHST- 2022/07/21 00:00 [accepted]
PHST- 2022/08/08 06:00 [pubmed]
PHST- 2022/08/30 06:00 [medline]
PHST- 2022/08/07 18:11 [entrez]
AID - S0042-6822(22)00123-4 [pii]
AID - 10.1016/j.virol.2022.07.012 [doi]
PST - ppublish
SO  - Virology. 2022 Sep;574:71-83. doi: 10.1016/j.virol.2022.07.012. Epub 2022 Jul 31.