PMID- 35934384
OWN - NLM
STAT- MEDLINE
DCOM- 20220809
LR  - 20220809
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1221
DP  - 2022 Aug 15
TI  - Combination of magnetic beads extraction and ultraperformance liquid 
      chromatography tandem mass spectrometry detection for the clinical diagnosis of 
      allergies.
PG  - 340157
LID - S0003-2670(22)00728-0 [pii]
LID - 10.1016/j.aca.2022.340157 [doi]
AB  - The total amount of immunoglobulin E (IgE) in human serum is an important 
      parameter in diagnosing allergies. To reduce the false diagnosis of allergies and 
      better assist in therapy, clinical studies can be performed to obtain accurate 
      and reliable measurements of IgE. A magnetic beads (MBs)-based ultraperformance 
      liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for total IgE 
      measurement and the diagnosis of food allergies in serum was developed in this 
      study. First, IgE was extracted by MBs coupled with anti-IgE antibody from serum. 
      The extracted IgE was quantified by a specific signal peptide after digestion. A 
      spiked linear IgE concentration ranging from 400 to 5000 ng mL(-1) was used for 
      quantification. The limits of detection and quantification were 400 ng mL(-1) and 
      800 ng mL(-1), respectively, for the developed method. Additionally, the combined 
      capacity of the extracted IgE with different allergic proteins was evaluated by a 
      binding experiment in vitro. The combining capacity of IgE with different 
      allergens was used to speculate the kind of allergens that induce allergies in 
      patients. Overall, a new method was developed that could be used to quantify the 
      amount of IgE and simultaneously diagnose which allergen causes an allergic 
      reaction, and this method may provide a powerful new tool in the clinical 
      detection of allergies. Moreover, the developed method was applied to analyze IgE 
      in four samples of patient serum and four serum samples from healthy individuals.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Li, Yang
AU  - Li Y
AD  - Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food 
      Poisoning, Beijing Center for Disease Prevention and Control, 100013, China; 
      School of Pharmaceutical Sciences, Health Science Center, Peking University, 
      100191, China.
FAU - Yang, Yi
AU  - Yang Y
AD  - Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food 
      Poisoning, Beijing Center for Disease Prevention and Control, 100013, China. 
      Electronic address: yybjcdc@163.com.
FAU - Liu, Yinghui
AU  - Liu Y
AD  - Shandong Qingdao Integrated Traditional Chinese and Western Medicine Hospital, 
      266002, China.
FAU - Liu, Jinyuan
AU  - Liu J
AD  - Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food 
      Poisoning, Beijing Center for Disease Prevention and Control, 100013, China.
FAU - Yang, Yunjia
AU  - Yang Y
AD  - Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food 
      Poisoning, Beijing Center for Disease Prevention and Control, 100013, China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food 
      Poisoning, Beijing Center for Disease Prevention and Control, 100013, China.
FAU - Zou, Yue
AU  - Zou Y
AD  - School of Pharmaceutical Sciences, Health Science Center, Peking University, 
      100191, China.
FAU - Shu, Lin
AU  - Shu L
AD  - School of Pharmaceutical Sciences, Health Science Center, Peking University, 
      100191, China.
FAU - Han, Nanyin
AU  - Han N
AD  - School of Pharmaceutical Sciences, Health Science Center, Peking University, 
      100191, China.
FAU - Shao, Bing
AU  - Shao B
AD  - Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food 
      Poisoning, Beijing Center for Disease Prevention and Control, 100013, China.
LA  - eng
PT  - Journal Article
DEP - 20220711
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - Chromatography, Liquid
MH  - Humans
MH  - *Hypersensitivity/diagnosis
MH  - Immunoglobulin E
MH  - Magnetic Phenomena
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Food allergy
OT  - IgE
OT  - Magnetic beads
OT  - UPLC-MS/MS
EDAT- 2022/08/08 06:00
MHDA- 2022/08/10 06:00
CRDT- 2022/08/07 21:04
PHST- 2022/02/04 00:00 [received]
PHST- 2022/06/01 00:00 [revised]
PHST- 2022/07/07 00:00 [accepted]
PHST- 2022/08/07 21:04 [entrez]
PHST- 2022/08/08 06:00 [pubmed]
PHST- 2022/08/10 06:00 [medline]
AID - S0003-2670(22)00728-0 [pii]
AID - 10.1016/j.aca.2022.340157 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2022 Aug 15;1221:340157. doi: 10.1016/j.aca.2022.340157. Epub 
      2022 Jul 11.