PMID- 35934425
OWN - NLM
STAT- MEDLINE
DCOM- 20220809
LR  - 20220809
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1222
DP  - 2022 Aug 22
TI  - "Three-in-one" nanohybrids as synergistic nanozymes assisted with exonuclease I 
      amplification to enhance colorimetric aptasensor for ultrasensitive detection of 
      kanamycin.
PG  - 340178
LID - S0003-2670(22)00749-8 [pii]
LID - 10.1016/j.aca.2022.340178 [doi]
AB  - Kanamycin (KAN) residues in animal-derived food can cause serious threats to 
      human health. Herein, a colorimetric aptasensor was described using 
      "three-in-one" nanohybrids (hemin@Fe-MIL-88NH(2)/PtNP) as synergistic nanozymes 
      assisted with the exonuclease I (Exo I) signal amplification for the 
      ultrasensitive detection of KAN. In the presence of KAN and Exo I, the KAN 
      aptamer (APT) was specifically bound to KAN, and the remaining APT complementary 
      strand (cDNA(1)) captured hemin@Fe-MIL-88NH(2)/PtNP labeled with the cDNA(1) 
      complementary strand (cDNA(2)). Due to the synergistic effect of hemin, 
      Fe-MIL-88NH(2) and platinum nanoparticles (PtNPs), hemin@Fe-MIL-88NH(2)/PtNP 
      exhibited superior catalytic performance and could efficiently catalyze the 
      3,3',5,5'-tetramethylbenzidine (TMB)-H(2)O(2) system for signal development. 
      Moreover, Exo I could digest APT and release KAN into a new cycle for signal 
      amplification. Based on multiple signal amplification effects, our proposed 
      aptasensor achieved a wide detection range from 0.01 to 100 ng mL(-1) with a low 
      detection limit of 2 pg mL(-1). This assay also successfully detected KAN-added 
      milk and shrimp samples with added recovery ranges of 93.58%-106.08% and relative 
      standard deviations (RSDs) of 2.20%-5.50%. The aptasensor enabled ultrasensitive, 
      specific, and fast detection of KAN, and exhibited promising applications in the 
      efficient detection of food pollutants.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Li, Guanghua
AU  - Li G
AD  - School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China; 
      Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment 
      and Food Safety, Tianjin Institute of Environment and Operational Medicine, 
      Tianjin, 300050, China.
FAU - Liu, Sha
AU  - Liu S
AD  - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment 
      and Food Safety, Tianjin Institute of Environment and Operational Medicine, 
      Tianjin, 300050, China.
FAU - Huo, Yapeng
AU  - Huo Y
AD  - Yantai Center for Disease Control and Prevention, Yantai, 264003, China.
FAU - Zhou, Huanying
AU  - Zhou H
AD  - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment 
      and Food Safety, Tianjin Institute of Environment and Operational Medicine, 
      Tianjin, 300050, China.
FAU - Li, Sen
AU  - Li S
AD  - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment 
      and Food Safety, Tianjin Institute of Environment and Operational Medicine, 
      Tianjin, 300050, China.
FAU - Lin, Xiaohui
AU  - Lin X
AD  - Tianjin Center for Disease Control and Prevention, Tianjin, 300050, China.
FAU - Kang, Weijun
AU  - Kang W
AD  - School of Public Health, Hebei Medical University, Shijiazhuang, 050017, China. 
      Electronic address: kangwj158@163.com.
FAU - Li, Shuang
AU  - Li S
AD  - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment 
      and Food Safety, Tianjin Institute of Environment and Operational Medicine, 
      Tianjin, 300050, China. Electronic address: Liza3320@163.com.
FAU - Gao, Zhixian
AU  - Gao Z
AD  - Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment 
      and Food Safety, Tianjin Institute of Environment and Operational Medicine, 
      Tianjin, 300050, China. Electronic address: gaozhx@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220719
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (DNA, Complementary)
RN  - 49DFR088MY (Platinum)
RN  - 59-01-8 (Kanamycin)
RN  - 743LRP9S7N (Hemin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.1.- (Exodeoxyribonucleases)
RN  - EC 3.1.11.1 (exodeoxyribonuclease I)
SB  - IM
MH  - Animals
MH  - *Aptamers, Nucleotide/chemistry
MH  - *Biosensing Techniques
MH  - Colorimetry
MH  - DNA, Complementary
MH  - Exodeoxyribonucleases
MH  - Hemin
MH  - Humans
MH  - Hydrogen Peroxide/chemistry
MH  - Kanamycin
MH  - Limit of Detection
MH  - *Metal Nanoparticles/chemistry
MH  - Platinum/chemistry
OTO - NOTNLM
OT  - Colorimetric aptasensor
OT  - Kanamycin
OT  - Nanohybrid
OT  - Nanozyme
OT  - Ultrasensitive detection
EDAT- 2022/08/08 06:00
MHDA- 2022/08/10 06:00
CRDT- 2022/08/07 21:05
PHST- 2022/05/10 00:00 [received]
PHST- 2022/07/13 00:00 [revised]
PHST- 2022/07/15 00:00 [accepted]
PHST- 2022/08/07 21:05 [entrez]
PHST- 2022/08/08 06:00 [pubmed]
PHST- 2022/08/10 06:00 [medline]
AID - S0003-2670(22)00749-8 [pii]
AID - 10.1016/j.aca.2022.340178 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2022 Aug 22;1222:340178. doi: 10.1016/j.aca.2022.340178. Epub 
      2022 Jul 19.