PMID- 35937577 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220809 IS - 2590-0064 (Electronic) IS - 2590-0064 (Linking) VI - 16 DP - 2022 Dec TI - 3D-printed high-density polyethylene scaffolds with bioactive and antibacterial layer-by-layer modification for auricle reconstruction. PG - 100361 LID - 10.1016/j.mtbio.2022.100361 [doi] LID - 100361 AB - High-density polyethylene (HDPE) is a promising material for the development of scaffold implants for auricle reconstruction. However, preparing a personalized HDPE auricle implant with favorable bioactive and antibacterial functions to promote skin tissue ingrowth is challenging. Herein, we present 3D-printed HDPE auricle scaffolds with satisfactory pore size and connectivity. The layer-by-layer (LBL) approach was applied to achieve the improved bioactive and antibacterial properties of these 3D printed scaffolds. The HDPE auricle scaffolds were fabricated using an extrusion 3D printing approach, and the individualized macrostructure and porous microstructure were both adjusted by the 3D printing parameters. The polydopamine (pDA) coating method was used to construct a multilayer epsilon-polylysine (EPL) and fibrin (FIB) modification on the surface of the 3D HDPE scaffold via the LBL self-assembly approach, which provides the bioactive and antibacterial properties. The results of the in vivo experiments using an animal model showed that LBL-coated HDPE auricular scaffolds were able to significantly enhance skin tissue ingrowth and ameliorate the inflammatory response caused by local stress. The results of this study suggest that the combination of the 3D printing technique and surface modification provides a promising strategy for developing personalized implants with biofunctional coatings, which show great potential as a scaffold implant for auricle reconstruction applications. CI - (c) 2022 The Authors. FAU - Yin, Junfeiyang AU - Yin J AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Zhong, Jing AU - Zhong J AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. AD - Dermatology Hospital, Southern Medical University, Guangzhou, 510091, China. FAU - Wang, Jiejie AU - Wang J AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Wang, Yilin AU - Wang Y AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Li, Ting AU - Li T AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Wang, Ling AU - Wang L AD - Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, 510515, China. FAU - Yang, Yang AU - Yang Y AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Zhen, Zhifang AU - Zhen Z AD - Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510000, China. FAU - Li, Yanbing AU - Li Y AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Zhang, Hongwu AU - Zhang H AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Zhong, Shizhen AU - Zhong S AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. AD - The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 030699, China. FAU - Wu, Yaobin AU - Wu Y AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. FAU - Huang, Wenhua AU - Huang W AD - Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China. AD - Guangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510000, China. AD - The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, 030699, China. LA - eng PT - Journal Article DEP - 20220715 PL - England TA - Mater Today Bio JT - Materials today. Bio JID - 101757228 PMC - PMC9352972 OTO - NOTNLM OT - 3D-printed high-density polyethylene OT - Antibacterial coating OT - Auricle reconstruction OT - Bioactive coating OT - Layer-by-layer approach COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2022/08/09 06:00 MHDA- 2022/08/09 06:01 PMCR- 2022/07/15 CRDT- 2022/08/08 04:04 PHST- 2022/05/09 00:00 [received] PHST- 2022/07/04 00:00 [revised] PHST- 2022/07/09 00:00 [accepted] PHST- 2022/08/08 04:04 [entrez] PHST- 2022/08/09 06:00 [pubmed] PHST- 2022/08/09 06:01 [medline] PHST- 2022/07/15 00:00 [pmc-release] AID - S2590-0064(22)00159-4 [pii] AID - 100361 [pii] AID - 10.1016/j.mtbio.2022.100361 [doi] PST - epublish SO - Mater Today Bio. 2022 Jul 15;16:100361. doi: 10.1016/j.mtbio.2022.100361. eCollection 2022 Dec.