PMID- 3593759
OWN - NLM
STAT- MEDLINE
DCOM- 19870805
LR  - 20220330
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 924
IP  - 3
DP  - 1987 Jun 22
TI  - The relative contributions of vitamin E, urate, ascorbate and proteins to the 
      total peroxyl radical-trapping antioxidant activity of human blood plasma.
PG  - 408-19
AB  - The Total (Peroxyl) Radical-trapping Antioxidant Parameter (TRAP) of six freshly 
      prepared human plasma samples and 45 frozen plasma samples has been determined. 
      It is shown that contributions from urate (35-65%), plasma proteins (10-50%), 
      ascorbate (0-24%) and vitamin E (5-10%) to TRAP account for all of the peroxyl 
      radical-trapping antioxidant activity in the majority of the samples. The changes 
      in concentrations of the plasma antioxidants during peroxyl radical attack show 
      that the first line of defense is provided by the plasma sulfhydryl groups, even 
      urate being spared during the initial stages of the reaction. The modes of action 
      of all of these plasma antioxidants and possible interactions between them are 
      discussed, with particular emphasis on the abilities of the water-soluble 
      antioxidants to regenerate or spare the only lipid-soluble antioxidant, vitamin 
      E.
FAU - Wayner, D D
AU  - Wayner DD
FAU - Burton, G W
AU  - Burton GW
FAU - Ingold, K U
AU  - Ingold KU
FAU - Barclay, L R
AU  - Barclay LR
FAU - Locke, S J
AU  - Locke SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Antioxidants)
RN  - 0 (Blood Proteins)
RN  - 0 (Free Radicals)
RN  - 0 (Lipid Peroxides)
RN  - 0 (Peroxides)
RN  - 1406-18-4 (Vitamin E)
RN  - 268B43MJ25 (Uric Acid)
RN  - 3170-83-0 (perhydroxyl radical)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Antioxidants/*blood
MH  - Ascorbic Acid/blood
MH  - Blood Proteins/physiology
MH  - Free Radicals
MH  - Humans
MH  - Lipid Peroxides/biosynthesis
MH  - Peroxides/*blood
MH  - Solubility
MH  - Uric Acid/blood
MH  - Vitamin E/blood
EDAT- 1987/06/22 00:00
MHDA- 1987/06/22 00:01
CRDT- 1987/06/22 00:00
PHST- 1987/06/22 00:00 [pubmed]
PHST- 1987/06/22 00:01 [medline]
PHST- 1987/06/22 00:00 [entrez]
AID - 0304-4165(87)90155-3 [pii]
AID - 10.1016/0304-4165(87)90155-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1987 Jun 22;924(3):408-19. doi: 
      10.1016/0304-4165(87)90155-3.