PMID- 35937804
OWN - NLM
STAT- MEDLINE
DCOM- 20220809
LR  - 20220826
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 13
DP  - 2022
TI  - NAT10 Maintains OGA mRNA Stability Through ac4C Modification in Regulating Oocyte 
      Maturation.
PG  - 907286
LID - 10.3389/fendo.2022.907286 [doi]
LID - 907286
AB  - In vitro maturation (IVM) refers to the process of developing immature oocytes 
      into the mature in vitro under the microenvironment analogous to follicle fluid. 
      It is an important technique for patients with polycystic ovary syndrome and, 
      especially, those young patients with the need of fertility preservation. 
      However, as the mechanisms of oocyte maturation have not been fully understood 
      yet, the cultivation efficiency of IVM is not satisfactory. It was confirmed in 
      our previous study that oocyte maturation was impaired after N-acetyltransferase 
      10 (NAT10) knockdown (KD). In the present study, we further explored the 
      transcriptome alteration of NAT10-depleted oocytes and found that 
      O-GlcNAcase(OGA) was an important target gene for NAT10-mediated ac4C 
      modification in oocyte maturation. NAT10 might regulate OGA stability and 
      expression by suppressing its degradation. To find out whether the influence of 
      NAT10-mediated ac4C on oocyte maturation was mediated by OGA, we further explored 
      the role of OGA in IVM. After knocking down OGA of oocytes, oocyte maturation was 
      inhibited. In addition, as oocytes matured, OGA expression increased and, 
      conversely, O-linked N-acetylglucosamine (O-GlcNAc) level decreased. On the basis 
      of NAT10 KD transcriptome and OGA KD transcriptome data, NAT10-mediated ac4C 
      modification of OGA might play a role through G protein-coupled receptors, 
      molecular transduction, nucleosome DNA binding, and other mechanisms in oocyte 
      maturation. Rsph6a, Gm7788, Gm41780, Trpc7, Gm29036, and Gm47144 were potential 
      downstream genes. In conclusion, NAT10 maintained the stability of OGA transcript 
      by ac4C modification on it, thus positively regulating IVM. Moreover, our study 
      revealed the regulation mechanisms of oocytes maturation and provided reference 
      for improving IVM outcomes. At the same time, the interaction between mRNA ac4C 
      modification and protein O-GlcNAc modification was found for the first time, 
      which enriched the regulation network of oocyte maturation.
CI  - Copyright (c) 2022 Lin, Xiang, Huang, Zeng, Zeng, Liu, Wu, Liang, Liang, Li and 
      Zhou.
FAU - Lin, Jiayu
AU  - Lin J
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Xiang, Yuting
AU  - Xiang Y
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Obstetrics and Gynecology, Affiliated Dongguan People's Hospital, 
      Southern Medical University, Dongguan, China.
FAU - Huang, Jiana
AU  - Huang J
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zeng, Haitao
AU  - Zeng H
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zeng, Yanyan
AU  - Zeng Y
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Liu, Jiawen
AU  - Liu J
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Wu, Taibao
AU  - Wu T
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Liang, Qiqi
AU  - Liang Q
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Liang, Xiaoyan
AU  - Liang X
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Li, Jingjie
AU  - Li J
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zhou, Chuanchuan
AU  - Zhou C
AD  - Reproductive Medicine Center, Sixth Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220722
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - V956696549 (Acetylglucosamine)
SB  - IM
MH  - Acetylglucosamine
MH  - Animals
MH  - Female
MH  - *Fertility Preservation
MH  - *Oocytes
MH  - RNA Stability
PMC - PMC9352860
OTO - NOTNLM
OT  - N4-acetylcytidine
OT  - NAT10
OT  - O-GlcNAc
OT  - OGA
OT  - in vitro maturation
OT  - oocyte
OT  - transcription
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/08/09 06:00
MHDA- 2022/08/10 06:00
PMCR- 2022/01/01
CRDT- 2022/08/08 04:08
PHST- 2022/03/29 00:00 [received]
PHST- 2022/05/16 00:00 [accepted]
PHST- 2022/08/08 04:08 [entrez]
PHST- 2022/08/09 06:00 [pubmed]
PHST- 2022/08/10 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2022.907286 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2022 Jul 22;13:907286. doi: 
      10.3389/fendo.2022.907286. eCollection 2022.