PMID- 35938100
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220809
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 9
DP  - 2022
TI  - Novel angiotensin-converting enzyme inhibitory peptides from tuna 
      byproducts-milts: Preparation, characterization, molecular docking study, and 
      antioxidant function on H(2)O(2)-damaged human umbilical vein endothelial cells.
PG  - 957778
LID - 10.3389/fnut.2022.957778 [doi]
LID - 957778
AB  - To prepare peptides with high angiotensin-converting enzyme (ACE) inhibitory 
      (ACEi) activity, Alcalase was screened from five proteases and employed to 
      prepare protein hydrolysate (TMH) of skipjack tuna (Katsuwonus pelamis) milts. 
      Subsequently, 10 novel ACEi peptides were isolated from the high-ACEi activity 
      TMH and identified as Tyr-Asp-Asp (YDD), Thr-Arg-Glu (TRE), Arg-Asp-Tyr (RDY), 
      Thr-Glu-Arg-Met (TERM), Asp-Arg-Arg-Tyr-Gly (DRRYG), Ile-Cys-Tyr (ICY), 
      Leu-Ser-Phe-Arg (LSFR), Gly-Val-Arg-Phe (GVRF), Lys-Leu-Tyr-Ala-Leu-Phe (KLYALF), 
      and Ile-Tyr-Ser-Pro (IYSP) with molecular weights of 411.35, 404.41, 452.45, 
      535.60, 665.69, 397.48, 521.61, 477.55, 753.91, and 478.53 Da, respectively. 
      Among them, the IC(50) values of ICY, LSFR, and IYSP on ACE were 0.48, 0.59, and 
      0.76 mg/mL, respectively. The significant ACEi activity of ICY, LSFR, and IYSP 
      with affinities of -7.0, -8.5, and -8.3 kcal/mol mainly attributed to effectively 
      combining with the ACEi active sites through hydrogen bonding, electrostatic 
      force, and hydrophobic interaction. Moreover, ICY, LSFR, and IYSP could 
      positively influence the production of nitric oxide (NO) and endothelin-1 (ET-1) 
      secretion in human umbilical vein endothelial cells (HUVECs) and weaken the 
      adverse impact of norepinephrine (NE) on the production of NO and ET-1. In 
      addition, ICY, LSFR, and IYSP could provide significant protection to HUVECs 
      against H(2)O(2) damage by increasing antioxidase levels to decrease the contents 
      of reactive oxide species and malondialdehyde. Therefore, the ACEi peptides of 
      ICY, LSFR, and IYSP are beneficial functional molecules for healthy foods against 
      hypertension and cardiovascular diseases.
CI  - Copyright (c) 2022 Suo, Zheng, Chi, Luo and Wang.
FAU - Suo, Shi-Kun
AU  - Suo SK
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College 
      of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan, China.
FAU - Zheng, Shuo-Lei
AU  - Zheng SL
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College 
      of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan, China.
FAU - Chi, Chang-Feng
AU  - Chi CF
AD  - National and Provincial Joint Laboratory of Exploration and Utilization of Marine 
      Aquatic Genetic Resources, National Engineering Research Center of Marine 
      Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean 
      University, Zhoushan, China.
FAU - Luo, Hong-Yu
AU  - Luo HY
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College 
      of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan, China.
FAU - Wang, Bin
AU  - Wang B
AD  - Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College 
      of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan, China.
LA  - eng
PT  - Journal Article
DEP - 20220722
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC9355146
OTO - NOTNLM
OT  - angiotensin-I-converting enzyme (ACE)
OT  - antihypertensive function
OT  - antioxidant activity
OT  - milt
OT  - peptide
OT  - skipjack tuna (Katsuwonus pelamis)
EDAT- 2022/08/09 06:00
MHDA- 2022/08/09 06:01
PMCR- 2022/01/01
CRDT- 2022/08/08 04:14
PHST- 2022/05/31 00:00 [received]
PHST- 2022/06/28 00:00 [accepted]
PHST- 2022/08/08 04:14 [entrez]
PHST- 2022/08/09 06:00 [pubmed]
PHST- 2022/08/09 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2022.957778 [doi]
PST - epublish
SO  - Front Nutr. 2022 Jul 22;9:957778. doi: 10.3389/fnut.2022.957778. eCollection 
      2022.