PMID- 35938503
OWN - NLM
STAT- MEDLINE
DCOM- 20220809
LR  - 20220811
IS  - 1744-5116 (Electronic)
IS  - 1388-0209 (Print)
IS  - 1388-0209 (Linking)
VI  - 60
IP  - 1
DP  - 2022 Dec
TI  - Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon 
      citrinus in rats fed with a high fat-fructose diet.
PG  - 1384-1393
LID - 10.1080/13880209.2022.2099907 [doi]
AB  - CONTEXT: Callistemon citrinus Skeels (Myrtaceae) exhibits many biological 
      activities. OBJECTIVE: This study analyzes for the first time, the toxicity, 
      obesogenic, and antioxidant effects of C. citrinus in rats fed with a high 
      fat-fructose diet (HFFD). MATERIALS AND METHODS: Four studies using male Wistar 
      rats were conducted: (a) 7 groups (n = 3): control (corn oil) and ethanol extract 
      of C. citrinus leaf (single oral dose at 100-4000 mg/kg) for acute toxicity; (b) 
      2 groups (n = 8): control (corn oil) and C. citrinus (1000 mg/kg/day) for 28 days 
      for subacute toxicity; (c) 3 groups (n = 4) with single oral dose of lipid 
      emulsion: control (lipid emulsion), C. citrinus and orlistat (250 and 50 mg/kg, 
      respectively) for lipid absorption; (d) 4 groups (n = 6): control (normal diet) 
      and 3 groups fed with HFFD: HFFD only, C. citrinus and simvastatin (oral dose 250 
      and 3 mg/kg, respectively) for 13 weeks. Antioxidant enzymes and biomarkers were 
      evaluated and inhibition of pancreatic lipase was determined in vitro. RESULTS: 
      Toxicological studies of C. citrinus showed no differences in biochemical 
      parameters and lethal dose (LD(50)) was higher than 4000 mg/kg. C. citrinus 
      inhibited pancreatic lipase activity, with IC(50) of 392.00 microg/mL, and decreased 
      lipid absorption by 70%. Additionally, it reduced the body weight 22%, restored 
      the activities of antioxidant enzymes, and reduced the biomarkers of oxidative 
      stress. CONCLUSIONS: Callistemon citrinus showed an effect against oxidative 
      stress by reducing biomarkers and induced antioxidant system, without toxic 
      effects.
FAU - Ortega-Perez, Luis Gerardo
AU  - Ortega-Perez LG
AD  - Facultad de Biologia, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, 
      Michoacan, Mexico.
FAU - Pinon-Simental, Jonathan Saul
AU  - Pinon-Simental JS
AD  - Facultad de Biologia, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, 
      Michoacan, Mexico.
FAU - Magana-Rodriguez, Oliver Rafid
AU  - Magana-Rodriguez OR
AD  - Facultad de Biologia, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, 
      Michoacan, Mexico.
FAU - Lopez-Mejia, Alejandro
AU  - Lopez-Mejia A
AD  - Facultad de Biologia, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, 
      Michoacan, Mexico.
FAU - Ayala-Ruiz, Luis Alberto
AU  - Ayala-Ruiz LA
AD  - Facultad de Biologia, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, 
      Michoacan, Mexico.
FAU - Garcia-Calderon, Aram Josue
AU  - Garcia-Calderon AJ
AD  - Facultad de Biologia, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, 
      Michoacan, Mexico.
FAU - Godinez-Hernandez, Daniel
AU  - Godinez-Hernandez D
AD  - Instituto de Investigaciones Quimico Biologicas, Universidad Michoacana de San 
      Nicolas de Hidalgo, Morelia, Michoacan, Mexico.
FAU - Rios-Chavez, Patricia
AU  - Rios-Chavez P
AUID- ORCID: 0000-0003-4285-8307
AD  - Facultad de Biologia, Universidad Michoacana de San Nicolas de Hidalgo, Morelia, 
      Michoacan, Mexico.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Pharm Biol
JT  - Pharmaceutical biology
JID - 9812552
RN  - 0 (Antioxidants)
RN  - 0 (Biomarkers)
RN  - 0 (Emulsions)
RN  - 30237-26-4 (Fructose)
RN  - 8001-30-7 (Corn Oil)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Animals
MH  - *Antioxidants/pharmacology
MH  - Biomarkers
MH  - Corn Oil
MH  - Diet, High-Fat/adverse effects
MH  - Emulsions
MH  - Fructose/toxicity
MH  - Lipase
MH  - Male
MH  - *Myrtaceae
MH  - Rats
MH  - Rats, Wistar
PMC - PMC9361760
OTO - NOTNLM
OT  - Alternative medicine
OT  - kidney
OT  - liver
OT  - overweight
OT  - oxidative stress
OT  - stomach
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/08/09 06:00
MHDA- 2022/08/10 06:00
PMCR- 2022/08/07
CRDT- 2022/08/08 05:43
PHST- 2022/08/08 05:43 [entrez]
PHST- 2022/08/09 06:00 [pubmed]
PHST- 2022/08/10 06:00 [medline]
PHST- 2022/08/07 00:00 [pmc-release]
AID - 2099907 [pii]
AID - 10.1080/13880209.2022.2099907 [doi]
PST - ppublish
SO  - Pharm Biol. 2022 Dec;60(1):1384-1393. doi: 10.1080/13880209.2022.2099907.