PMID- 35939894
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220823
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 276
DP  - 2022 Sep
TI  - Combined in vitro and in vivo investigation of barite microcrystals in Spirogyra 
      (Zygnematophyceae, Charophyta).
PG  - 153769
LID - S0176-1617(22)00155-9 [pii]
LID - 10.1016/j.jplph.2022.153769 [doi]
AB  - We have investigated the biomineralisation of barite ‒a useful proxy for 
      reconstructing paleoproductivity‒ in a freshwater alga, Spirogyra, by combining 
      in vitro and in vivo approaches to unveil the nature of its barite microcrystals. 
      Scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy 
      (EDXS) observations on simply dried samples revealed that the number and size of 
      barite crystals were related to the barium concentration in the media. 
      Additionally, their morphology showed a crystallographic face (011), which is not 
      normally observed, suggesting the influence of organic molecules on the growth 
      kinetics. The critical point drying method was used to preserve the internal and 
      external structures of Spirogyra cells for SEM imaging. Crystals were found 
      adjacent to the cytoplasmic membrane, near chloroplasts and fibrillary network. 
      In vivo optical microscopy and Raman tweezer microspectroscopy in living cells 
      showed that barite microcrystals are optically visible and follow cytoplasmic 
      streaming. These results led us to propose that barite formation in Spirogyra 
      occurs in the cytoplasm where barium and sulphate are both available: barium 
      supplied non-selectively through the active transport of the divalent cations 
      needed for actin polymerisation, and sulphate because necessary for amino acid 
      biosynthesis in chloroplasts.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier GmbH.. All rights reserved.
FAU - Barbosa, Natercia
AU  - Barbosa N
AD  - Department F.-A. Forel, University of Geneva, Boulevard Carl-Vogt 66, CH-1205 
      Geneva, Switzerland; Department of Physical Chemistry, University of Geneva, Quai 
      Ernest-Ansermet 30, CH-1205 Geneva, Switzerland.
FAU - Jaquet, Jean-Michel
AU  - Jaquet JM
AD  - Department Earth Sciences, University of Geneva, Rue des Maraichers 13, CH-1205 
      Geneva, Switzerland.
FAU - Urquidi, Oscar
AU  - Urquidi O
AD  - Department of Physical Chemistry, University of Geneva, Quai Ernest-Ansermet 30, 
      CH-1205 Geneva, Switzerland.
FAU - Adachi, Takuji B M
AU  - Adachi TBM
AD  - Department of Physical Chemistry, University of Geneva, Quai Ernest-Ansermet 30, 
      CH-1205 Geneva, Switzerland. Electronic address: Takuji.Adachi@unige.ch.
FAU - Filella, Montserrat
AU  - Filella M
AD  - Department F.-A. Forel, University of Geneva, Boulevard Carl-Vogt 66, CH-1205 
      Geneva, Switzerland. Electronic address: Montserrat.Filella@unige.ch.
LA  - eng
PT  - Journal Article
DEP - 20220716
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Sulfates)
RN  - 24GP945V5T (Barium)
RN  - 25BB7EKE2E (Barium Sulfate)
SB  - IM
MH  - Barium
MH  - Barium Sulfate/metabolism
MH  - *Charophyceae/metabolism
MH  - *Spirogyra/metabolism
MH  - Sulfates
OTO - NOTNLM
OT  - Barite
OT  - Barium sulphate
OT  - Biomineralisation
OT  - Raman tweezer microspectroscopy
OT  - SEM-EDXS
OT  - Spirogyra
EDAT- 2022/08/09 06:00
MHDA- 2022/08/24 06:00
CRDT- 2022/08/08 18:16
PHST- 2022/01/10 00:00 [received]
PHST- 2022/06/17 00:00 [revised]
PHST- 2022/07/11 00:00 [accepted]
PHST- 2022/08/09 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/08/08 18:16 [entrez]
AID - S0176-1617(22)00155-9 [pii]
AID - 10.1016/j.jplph.2022.153769 [doi]
PST - ppublish
SO  - J Plant Physiol. 2022 Sep;276:153769. doi: 10.1016/j.jplph.2022.153769. Epub 2022 
      Jul 16.