PMID- 35940082
OWN - NLM
STAT- MEDLINE
DCOM- 20221123
LR  - 20230130
IS  - 2452-3186 (Electronic)
IS  - 2452-3186 (Linking)
VI  - 70
IP  - 4
DP  - 2022 Sep
TI  - Exosomal miR-195 in hUC-MSCs alleviates hypoxia-induced damage of trophoblast 
      cells through tissue factor pathway inhibitor 2.
PG  - 103352
LID - S2452-3186(22)00020-4 [pii]
LID - 10.1016/j.retram.2022.103352 [doi]
AB  - MicroRNA-195 (miR-195) was decreased in the patients with pre-eclampsia (PE), 
      which was implicated to modulate PE. Moreover, tissue factor pathway inhibitor 2 
      (TFPI2), which was highly expressed in the placenta of PE patients, was 
      negatively correlated with miR-195 levels. This study aimed to explore the role 
      of miR-195 in the cell therapy for the treatment of PE and the underlying 
      mechanisms. Human umbilical cord mesenchymal stem cells (hUC-MSCs) were 
      transfected with miR-195 mimic or mimic negative control to extract exosomes. 
      HTR8/SVneo was incubated under hypoxia condition to induce cell damage, and 
      co-co-cultured with exosomes derived from hUC-MSCs to evaluate its effect. 
      Hypoxia time-dependently caused a decrease on miR-195 level with an increase on 
      TFPI2 expression in HTR8/SVneo. MiR-195 directly bind to TFPI2 and inhibited 
      TFPI2 expression in hUC-MSCs. Moreover, hypoxia-induced cell damage in HTR8/SVneo 
      was significantly attenuated by co-culture with hUC-MSC-derived exosomes. 
      Exosomes extracted from miR-195-overexpressed hUC-MSCs, could further ameliorate 
      hypoxia-induced cell damage, due to the excessive amount of miR-195 delivered by 
      exosomes. Exosomal miR-195 in hUC-MSCs alleviated hypoxia-induced cell damage 
      through TFPI2, which might provide a potential therapeutic approach for 
      pre-eclampsia.
CI  - Copyright (c) 2022 Elsevier Masson SAS. All rights reserved.
FAU - Zhou, Chenchen
AU  - Zhou C
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China.
FAU - Wan, Sheng
AU  - Wan S
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China.
FAU - Zhao, Xiaobo
AU  - Zhao X
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China.
FAU - Gu, Shengyi
AU  - Gu S
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China.
FAU - Pei, Jindan
AU  - Pei J
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China.
FAU - Wu, Yuelin
AU  - Wu Y
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China.
FAU - Han, Zhimin
AU  - Han Z
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China.
FAU - Che, Ronghua
AU  - Che R
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China. Electronic address: 
      crh73@51mch.com.
FAU - Hua, Xiaolin
AU  - Hua X
AD  - Department of Obstetrics, Shanghai First Maternity and Infant Hospital, School of 
      Medicine, Tongji University, Shanghai 200092, China. Electronic address: 
      xiaolin_hua@tongji.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220805
PL  - France
TA  - Curr Res Transl Med
JT  - Current research in translational medicine
JID - 101681234
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN195 microRNA, human)
RN  - 0 (tissue-factor-pathway inhibitor 2)
RN  - 0 (Glycoproteins)
SB  - IM
MH  - Female
MH  - Humans
MH  - Pregnancy
MH  - Hypoxia
MH  - *Mesenchymal Stem Cells
MH  - *MicroRNAs/genetics
MH  - *Pre-Eclampsia/genetics/therapy
MH  - Trophoblasts
MH  - *Glycoproteins/genetics
OTO - NOTNLM
OT  - Exosome
OT  - Hypoxia
OT  - PE
OT  - TFPI2
OT  - miR-195
COIS- Declaration of Competing Interest None declared.
EDAT- 2022/08/09 06:00
MHDA- 2022/11/23 06:00
CRDT- 2022/08/08 18:31
PHST- 2021/09/29 00:00 [received]
PHST- 2022/04/23 00:00 [revised]
PHST- 2022/05/17 00:00 [accepted]
PHST- 2022/08/09 06:00 [pubmed]
PHST- 2022/11/23 06:00 [medline]
PHST- 2022/08/08 18:31 [entrez]
AID - S2452-3186(22)00020-4 [pii]
AID - 10.1016/j.retram.2022.103352 [doi]
PST - ppublish
SO  - Curr Res Transl Med. 2022 Sep;70(4):103352. doi: 10.1016/j.retram.2022.103352. 
      Epub 2022 Aug 5.