PMID- 35941691
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220811
IS  - 1758-5996 (Print)
IS  - 1758-5996 (Electronic)
IS  - 1758-5996 (Linking)
VI  - 14
IP  - 1
DP  - 2022 Aug 8
TI  - Combined analysis of whole-exome sequencing and RNA sequencing in type 2 diabetes 
      mellitus patients with thirst and fatigue.
PG  - 111
LID - 10.1186/s13098-022-00884-z [doi]
LID - 111
AB  - BACKGROUND: The principal objective of this study was to gain a better 
      understanding of the mechanisms of type 2 diabetes mellitus (T2DM) patients with 
      fatigue (D-T2DM) through exome and transcriptome sequencing. METHODS: After 
      whole-exome sequencing on peripheral blood of 6 D-T2DM patients, the consensus 
      mutations were screen out and analyzed by a series of bioinformatics analyses. 
      Then, we combined whole-exome sequencing and transcriptome sequencing results to 
      find the important genes that changed at both the DNA and RNA levels. RESULTS: 
      The results showed that a total of 265,393 mutation sites were found in D-T2DM 
      patients compared with normal individuals, 235 of which were consensus mutations 
      shared with D-T2DM patients. These genes significantly enriched in HIF-1 
      signaling pathway and sphingolipid signaling pathway. At the RNA level, a total 
      of 375 genes were identified to be differentially expressed. After the DNA-RNA 
      joint analysis, eight genes were screened that changed at both DNA and RNA 
      levels. Among these genes, FUS and LMNA were related to carbohydrate metabolism, 
      energy metabolism, and mitochondrial function. Subsequently, we predicted the 
      herbs, including Qin Pi and Hei Zhi Ma, that might play a therapeutic role in 
      D-T2DM through the SymMap database. CONCLUSION: These findings have significant 
      implications for understanding the mechanisms of D-T2DM and provide potential 
      targets for D-T2DM diagnosis and treatment.
CI  - (c) 2022. The Author(s).
FAU - Lv, Bohan
AU  - Lv B
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Yang, Xiuyan
AU  - Yang X
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - An, Tian
AU  - An T
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Wu, Yanxiang
AU  - Wu Y
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - He, Zhongchen
AU  - He Z
AD  - Department of Endocrinology, Beijing He Ping li Hospital, Beijing, China.
FAU - Li, Bowu
AU  - Li B
AD  - Department of Endocrinology, Beijing He Ping li Hospital, Beijing, China.
FAU - Wang, Yijiao
AU  - Wang Y
AD  - Department of Endocrinology, Beijing He Ping li Hospital, Beijing, China.
FAU - Tan, Fang
AU  - Tan F
AD  - Department of Endocrinology, Beijing He Ping li Hospital, Beijing, China.
FAU - Wang, Tingye
AU  - Wang T
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Zhu, Jiajian
AU  - Zhu J
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Hu, Yuanyuan
AU  - Hu Y
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China.
FAU - Liu, Xiaokun
AU  - Liu X
AD  - Department of Cardiology, Gongren Hospital of Tangshan City, Tangshan, China. 
      lxiaokun@sohu.com.
FAU - Jiang, Guangjian
AU  - Jiang G
AD  - Traditional Chinese Medicine School, Beijing University of Chinese Medicine, 
      Beijing, China. bucmjiang@163.com.
LA  - eng
GR  - NSFC81774171/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220808
PL  - England
TA  - Diabetol Metab Syndr
JT  - Diabetology & metabolic syndrome
JID - 101488958
PMC - PMC9358875
OTO - NOTNLM
OT  - Diabetes
OT  - Fatigue
OT  - Mitochondria
OT  - RNA sequencing
OT  - Whole-exome sequencing
COIS- The authors declare that they have no competing interests.
EDAT- 2022/08/09 06:00
MHDA- 2022/08/09 06:01
PMCR- 2022/08/08
CRDT- 2022/08/08 23:53
PHST- 2022/02/26 00:00 [received]
PHST- 2022/07/28 00:00 [accepted]
PHST- 2022/08/08 23:53 [entrez]
PHST- 2022/08/09 06:00 [pubmed]
PHST- 2022/08/09 06:01 [medline]
PHST- 2022/08/08 00:00 [pmc-release]
AID - 10.1186/s13098-022-00884-z [pii]
AID - 884 [pii]
AID - 10.1186/s13098-022-00884-z [doi]
PST - epublish
SO  - Diabetol Metab Syndr. 2022 Aug 8;14(1):111. doi: 10.1186/s13098-022-00884-z.